Λεπτομέρειες βιβλιογραφικής εγγραφής
| Τίτλος: |
Multimodal forensic biomarkers of death: gene expression, enzyme kinetics, and histology for PMI estimation. |
| Συγγραφείς: |
Gadepally, Sai Soumya1 (AUTHOR), Thaker, Khushali1 (AUTHOR), Patoliya, Jaimini1 (AUTHOR), Kubhchandani, Harish T.2 (AUTHOR), Joshi, Rushikesh1 (AUTHOR) rushikeshjoshi@gujaratuniversity.ac.in |
| Πηγή: |
Australian Journal of Forensic Sciences. Oct2026, Vol. 58 Issue 5, p530-545. 16p. |
| Θεματικοί όροι: |
*Gene expression, *Enzyme kinetics, *Glutathione peroxidase, *Biomarkers, *Histology, *Oxidative stress, *Glutathione reductase |
| Περίληψη: |
Accurate estimation of the post-mortem interval (PMI) is essential in forensic medicine, and thanatochemistry provides valuable biochemical insights into post-mortem changes. This study investigates the potential of oxidative stress-related gene expression, enzymatic activity, and histological changes in liver tissues of Wistar rats (Mus musculus) and humans as reliable PMI markers. Using microarray-based gene expression profiling, transcriptomic alterations at varying PMIs were analysed, focusing on genes associated with oxidative stress. Differential gene expression was validated through enzymatic assays quantifying glutathione reductase (GSR) and glutathione peroxidase (GPx1) using UV spectroscopy. Additionally, histological examinations of liver tissue revealed progressive morphological changes correlating with increasing PMI. Results demonstrated significant temporal variations in GSR and GPx1 expression and activity, with corresponding structural degradation in liver tissues. These findings suggest that a combined analysis of transcriptomic, enzymatic, and histological parameters can enhance the accuracy of PMI estimation in forensic investigations. [ABSTRACT FROM AUTHOR] |
| Βάση Δεδομένων: |
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