Λεπτομέρειες βιβλιογραφικής εγγραφής
| Τίτλος: |
Chemometric-Assisted UV-Vis Spectroscopy for the Simultaneous Determination of Metformin and Glibenclamide: Overcoming Extreme Mass Ratios and Spectral Congestion via Multivariate Calibration. |
| Συγγραφείς: |
Carvalho, Ânderson Ramos1 (AUTHOR), Machioli, Lilian Fanfa2 (AUTHOR), Carlos, Graciela2 (AUTHOR), Virginia Garcia, Cássia2 (AUTHOR), Ferrão, Marco Flôres1,3 (AUTHOR), Steppe, Martin2 (AUTHOR), Sebastian Loureiro Mendez, Andreas2 (AUTHOR) andreas.mendez@ufrgs.br |
| Πηγή: |
Analytical Letters. Jun2026, p1-16. 16p. 4 Illustrations. |
| Θεματικοί όροι: |
*Metformin, *Glibenclamide, *Hypoglycemic agents, *Partial least squares regression, *Drug analysis, *Chemometrics, *Multivariate analysis, *Ultraviolet-visible spectroscopy |
| Περίληψη: |
AbstractThe simultaneous quantification of metformin (MET) and glibenclamide (GLB) in fixed-dose pharmaceutical formulations remains analytically challenging due to the pronounced concentration disparity between the drugs and the extensive spectral overlap in the ultraviolet region. This study describes the development of a UV-Vis spectrophotometric method combined with partial least squares (PLS) regression and principal component analysis (PCA) for the simultaneous determination of MET and GLB without chromatographic separation. Three multivariate calibration strategies were evaluated, including MET-priority (200–400 nm), GLB-priority (250–400 nm), and a global model optimized by variable selection. The optimized PLS models showed satisfactory predictive performance, with R2pred values ≥ 0.99 and root mean square error of prediction (RMSEP) values ≤ 3 µg/mL for the selected models. The proposed methodology was successfully applied to commercial pharmaceutical formulations, including samples presenting significant matrix effects associated with high MET:GLB ratios. Validation parameters were evaluated according to analytical performance principles commonly applied to chemometric calibration methods, including precision, accuracy, robustness, and external prediction capability. In comparison with conventional high-performance liquid chromatography based assays reported in the literature, the proposed UV-Vis/PLS approach required substantially lower solvent consumption and reduced analytical complexity, supporting its applicability for routine quality-control analysis of antidiabetic formulations. [ABSTRACT FROM AUTHOR] |
| Βάση Δεδομένων: |
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