Λεπτομέρειες βιβλιογραφικής εγγραφής
| Τίτλος: |
Accessible and cost-effective deployment of environmental DNA (eDNA) samplers for sediment conducive to supporting community-based surveys. |
| Συγγραφείς: |
Dema, Anna H.1 (AUTHOR), Crichton, Ellika M.1 (AUTHOR), Acharya-Patel, Neha1 (AUTHOR), Bergman, Lauren C.1 (AUTHOR), Allison, Michael J.1 (AUTHOR), Bonderud, Matthew T.1 (AUTHOR), Imbery, Jacob J.1 (AUTHOR), Robinson, Clifford L.K.2 (AUTHOR), Huard, Jacqueline R.3 (AUTHOR), Helbing, Caren C.1 (AUTHOR) chelbing@uvic.ca |
| Πηγή: |
PLoS ONE. 2/24/2026, Vol. 21 Issue 2, p1-15. 15p. |
| Θεματικοί όροι: |
*Sediment sampling, *Environmental monitoring, *Environmental sampling, *Nucleic acids |
| Γεωγραφικοί όροι: |
Pacific Coast (B.C.) |
| Περίληψη: |
The presence of at-risk, invasive, and sentinel species are measures of biodiversity, however it is often challenging to quickly gather reliable data through conventional, time-constrained surveying techniques. Environmental DNA (eDNA) detection is one method that has proven to be extremely useful for biomonitoring, particularly due to its non-invasive nature, cost efficiency, sensitivity, accuracy, and relative ease to carry out in the field. Conventional sediment sampling presents a challenge to obtain suitable representative samples and there is a need for easily accessible methods that are compatible with community-based monitoring activities and budgets. Herein, we introduce a "FloppE-Dip" method, a passive sampling approach that is compatible with larger sand sediment sample volumes up to 180 mL and utilizes easily accessible materials. We compared the FloppE-Dip method to conventional filtration through the detection of several species' DNA from environmental samples using real-time quantitative PCR (qPCR). In a laboratory study using an American bullfrog (Rana [Lithobates] catesbeiana) tissue slurry, we determined optimal protocol parameters that were then applied in a field survey to identify beaches in coastal British Columbia used by the important forage fish, the Pacific sand lance (Ammodytes personatus). Of the 20 sampling sites, both FloppE-Dip and conventional filtration methods detected Pacific sand lance eDNA at 14 of them, albeit FloppE-Dip copies/L estimates were typically 5–10 times lower. Two sites returned no detections for both methods, and four sites returned low detections using conventional filtration methods where the FloppE-Dip method showed no detection. This discrepancy at low copy numbers may be rectified through increasing the number of samples taken per site and/or increasing the number of technical replicates. Overall, the FloppE-Dip method was more reliable than visual observation and is considerably faster and cheaper than filtration making it well-suited for general detection purposes. While other sediment types remain to be tested, the simplicity, efficiency, and use of readily available materials make FloppE-Dip a viable alternative for community-based monitoring programs, particularly given the time and budget constraints these programs often face. [ABSTRACT FROM AUTHOR] |
| Βάση Δεδομένων: |
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