Bibliographic Details
| Title: |
Immobilization of urease-containing liposomes in microbeads: an approach to recover and reuse a liposomal enzyme. |
| Authors: |
Okano, Shinya1 (AUTHOR), Yoshimoto, Makoto1 (AUTHOR) yosimoto@yamaguchi-u.ac.jp |
| Source: |
Journal of Dispersion Science & Technology. Dec2025, p1-9. 9p. 6 Illustrations. |
| Subject Terms: |
*Urease, *Liposomes, *Molecular capsules, *Enzyme inactivation, *Immobilized enzymes, *Catalytic hydrolysis, *Microspheres |
| Abstract: |
AbstractImmobilization of phospholipid vesicles (liposomes) in a carrier material offers opportunities to recover and reuse liposome-encapsulated enzymes. In the present work, urease-containing liposomes were prepared and immobilized onto polystyrene beads through the binding between a biotinylated lipid incorporated in liposome membranes and a neutralized avidin covalently conjugated to the bead. Liposome-encapsulated urease catalyzed the hydrolysis of urea in a Tris-HCl buffer solution (pH = 8.0) at 25 °C because urea could pass through lipid bilayers. Immobilized urease-containing liposomes were storable at 4 °C and used repeatedly twenty times for catalyzing the hydrolysis of urea for 40 min giving the total reaction time of 800 min. Enzyme activity of the catalyst decreased in the first and second reactions but the activity was stable from third to twentieth reactions. The results obtained demonstrate that urease in its biologically active form can be immobilized in polystyrene beads via liposomes for the repeated catalytic hydrolysis of urea. [ABSTRACT FROM AUTHOR] |
| Database: |
Academic Search Index |