Isolation and RAPD analysis of DNA from pineneedles of Pinus pinea of three greek populations

After the initial work dealing with DNA isolation for plant and especially for conifers, only few efforts were made to develop new techniques. In this work, DNA was isolated using a method that does not require liquid nitrogen. Methodology/Results: The object of this work was examination of genetic...

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Κύριος συγγραφέας:	Τσικλιτά, Καλλιόπη
Άλλοι συγγραφείς:	Παπαγεωργίου, Αριστοτέλης
Γλώσσα:	English
Δημοσίευση:	2015
Θέματα:	Pinus pinea Απομόνωση DNA Γενετική στένωση DNA isolation Bottleneck effect
Διαθέσιμο Online:	https://vsmart.lib.aegean.gr/webopac/List.csp?SearchT1=%CE%A4%CF%83%CE%B9%CE%BA%CE%BB%CE%B9%CF%84%CE%AC%2C+%CE%9A%CE%B1%CE%BB%CE%BB%CE%B9%CF%8C%CF%80%CE%B7&Index1=Keywordsbib&Database=1&SearchMethod=Find_1&SearchTerm1=%CE%A4%CF%83%CE%B9%CE%BA%CE%BB%CE%B9%CF%84%CE%AC%2C+%CE%9A%CE%B1%CE%BB%CE%BB%CE%B9%CF%8C%CF%80%CE%B7&OpacLanguage=gre&Profile=Default&EncodedRequest=C694oEAD0S9723130D3C11E1D1C612&EncodedQuery=C694oEAD0S9723130D3C11E1D1C612&Source=SysQR&PageType=Start&PreviousList=RecordListFind&WebPageNr=1&NumberToRetrieve=50&WebAction=NewSearch&StartValue=0&RowRepeat=0&ExtraInfo=&SortIndex=Year&SortDirection=-1&Resource=&SavingIndicator=&RestrType=&RestrTerms=&RestrShowAll=&LinkToIndex= http://hdl.handle.net/11610/11675
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author	Τσικλιτά, Καλλιόπη
author2	Παπαγεωργίου, Αριστοτέλης
author_facet	Παπαγεωργίου, Αριστοτέλης Τσικλιτά, Καλλιόπη
author_sort	Τσικλιτά, Καλλιόπη
collection	DSpace
description	After the initial work dealing with DNA isolation for plant and especially for conifers, only few efforts were made to develop new techniques. In this work, DNA was isolated using a method that does not require liquid nitrogen. Methodology/Results: The object of this work was examination of genetic diversity of three Greek populations of stone pine. DNA was isolated from healthy pineneedles using the isolation protocol Charge Switch gDNA Plant Kit of Invitrogen. Exsiccation with silicagel was the key for successful results for a resilient plant such as Pinus pinea. For molecular analysis two primers were used in polymerase chain reaction, OPA04 and OPU14. Each DNA segment that was detected constitutes a RAPD marker. Presence or absence of marker was digitally encoded in binary system and represented with unit and zero respectively. According to the results a phylogenetic analysis dendrogram was produced using UPGMA method based on DICE marker. RAPD analysis showed similarities between the populations of Marathonas and Kotuchi, and their divergence from the population of Skiathos. Conclusions: The results can be useful for proposing new methodologies for genetic identification of other species of pine. The modified technique for DNA isolation using exsiccation with silicagel was successfully applied in this study and the RAPD technique may serve as an efficient tool for further molecular studies.
id	oai:hellanicus.lib.aegean.gr:11610-11675
institution	Hellanicus
language	English
publishDate	2015
record_format	dspace
spelling	oai:hellanicus.lib.aegean.gr:11610-116752021-01-25T07:39:20Z Isolation and RAPD analysis of DNA from pineneedles of Pinus pinea of three greek populations Τσικλιτά, Καλλιόπη Παπαγεωργίου, Αριστοτέλης Pinus pinea Απομόνωση DNA Γενετική στένωση DNA isolation Bottleneck effect After the initial work dealing with DNA isolation for plant and especially for conifers, only few efforts were made to develop new techniques. In this work, DNA was isolated using a method that does not require liquid nitrogen. Methodology/Results: The object of this work was examination of genetic diversity of three Greek populations of stone pine. DNA was isolated from healthy pineneedles using the isolation protocol Charge Switch gDNA Plant Kit of Invitrogen. Exsiccation with silicagel was the key for successful results for a resilient plant such as Pinus pinea. For molecular analysis two primers were used in polymerase chain reaction, OPA04 and OPU14. Each DNA segment that was detected constitutes a RAPD marker. Presence or absence of marker was digitally encoded in binary system and represented with unit and zero respectively. According to the results a phylogenetic analysis dendrogram was produced using UPGMA method based on DICE marker. RAPD analysis showed similarities between the populations of Marathonas and Kotuchi, and their divergence from the population of Skiathos. Conclusions: The results can be useful for proposing new methodologies for genetic identification of other species of pine. The modified technique for DNA isolation using exsiccation with silicagel was successfully applied in this study and the RAPD technique may serve as an efficient tool for further molecular studies. 2015-11-18T09:46:03Z 2015-11-18T09:46:03Z 2009 https://vsmart.lib.aegean.gr/webopac/List.csp?SearchT1=%CE%A4%CF%83%CE%B9%CE%BA%CE%BB%CE%B9%CF%84%CE%AC%2C+%CE%9A%CE%B1%CE%BB%CE%BB%CE%B9%CF%8C%CF%80%CE%B7&Index1=Keywordsbib&Database=1&SearchMethod=Find_1&SearchTerm1=%CE%A4%CF%83%CE%B9%CE%BA%CE%BB%CE%B9%CF%84%CE%AC%2C+%CE%9A%CE%B1%CE%BB%CE%BB%CE%B9%CF%8C%CF%80%CE%B7&OpacLanguage=gre&Profile=Default&EncodedRequest=C694oEAD0S9723130D3C11E1D1C612&EncodedQuery=C694oEAD0S9723130D3C11E1D1C612&Source=SysQR&PageType=Start&PreviousList=RecordListFind&WebPageNr=1&NumberToRetrieve=50&WebAction=NewSearch&StartValue=0&RowRepeat=0&ExtraInfo=&SortIndex=Year&SortDirection=-1&Resource=&SavingIndicator=&RestrType=&RestrTerms=&RestrShowAll=&LinkToIndex= http://hdl.handle.net/11610/11675 en Μυτιλήνη
spellingShingle	Pinus pinea Απομόνωση DNA Γενετική στένωση DNA isolation Bottleneck effect Τσικλιτά, Καλλιόπη Isolation and RAPD analysis of DNA from pineneedles of Pinus pinea of three greek populations
title	Isolation and RAPD analysis of DNA from pineneedles of Pinus pinea of three greek populations
title_full	Isolation and RAPD analysis of DNA from pineneedles of Pinus pinea of three greek populations
title_fullStr	Isolation and RAPD analysis of DNA from pineneedles of Pinus pinea of three greek populations
title_full_unstemmed	Isolation and RAPD analysis of DNA from pineneedles of Pinus pinea of three greek populations
title_short	Isolation and RAPD analysis of DNA from pineneedles of Pinus pinea of three greek populations
title_sort	isolation and rapd analysis of dna from pineneedles of pinus pinea of three greek populations
topic	Pinus pinea Απομόνωση DNA Γενετική στένωση DNA isolation Bottleneck effect
url	https://vsmart.lib.aegean.gr/webopac/List.csp?SearchT1=%CE%A4%CF%83%CE%B9%CE%BA%CE%BB%CE%B9%CF%84%CE%AC%2C+%CE%9A%CE%B1%CE%BB%CE%BB%CE%B9%CF%8C%CF%80%CE%B7&Index1=Keywordsbib&Database=1&SearchMethod=Find_1&SearchTerm1=%CE%A4%CF%83%CE%B9%CE%BA%CE%BB%CE%B9%CF%84%CE%AC%2C+%CE%9A%CE%B1%CE%BB%CE%BB%CE%B9%CF%8C%CF%80%CE%B7&OpacLanguage=gre&Profile=Default&EncodedRequest=C694oEAD0S9723130D3C11E1D1C612&EncodedQuery=C694oEAD0S9723130D3C11E1D1C612&Source=SysQR&PageType=Start&PreviousList=RecordListFind&WebPageNr=1&NumberToRetrieve=50&WebAction=NewSearch&StartValue=0&RowRepeat=0&ExtraInfo=&SortIndex=Year&SortDirection=-1&Resource=&SavingIndicator=&RestrType=&RestrTerms=&RestrShowAll=&LinkToIndex= http://hdl.handle.net/11610/11675
work_keys_str_mv	AT tsiklitakalliopē isolationandrapdanalysisofdnafrompineneedlesofpinuspineaofthreegreekpopulations

Isolation and RAPD analysis of DNA from pineneedles of Pinus pinea of three greek populations

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