Academic Journal
A multiscale modeling approach to study the role of mechanics and inflammation in the pathophysiology of articular cartilage.
| Τίτλος: | A multiscale modeling approach to study the role of mechanics and inflammation in the pathophysiology of articular cartilage. |
|---|---|
| Συγγραφείς: | Mukherjee, Satanik, Lesage, Raphaelle, Geris, Liesbet |
| Πηγή: | Computers in Biology and Medicine, 211, 111737 (2026-07-15) |
| Στοιχεία εκδότη: | Elsevier Ltd, 2026. |
| Έτος έκδοσης: | 2026 |
| Θεματικοί όροι: | Articular cartilage, Finite element analysis, Gene regulatory network, Mechanotransduction, Multiscale modeling, Osteoarthritis, Animals, Humans, Finite Element Analysis, Computer Simulation, Stress, Mechanical, Cartilage, Articular/physiopathology, Cartilage, Articular/metabolism, Cartilage, Articular/pathology, Models, Biological, Osteoarthritis/physiopathology, Osteoarthritis/metabolism, Osteoarthritis/pathology, Chondrocytes/metabolism, Chondrocytes/pathology, Inflammation/physiopathology, Inflammation/metabolism, Inflammation/pathology, Mechanotransduction, Cellular, Articular cartilages, Chondrocytes, Finite element analyse, Finite element modelling (FEM), Gene regulatory networks, Length scale, Mechanical loading, Cartilage, Articular, Inflammation, Health Informatics, Computer Science Applications, Engineering, computing & technology, Ingénierie, informatique & technologie |
| Περιγραφή: | Mechanical loading regulates chondrocyte health in articular cartilage. While physiological stimuli maintain homeostasis, supra-physiological stimuli from joint injuries disrupt it, leading to osteoarthritis (OA). OA progression involves complex mechanical and biochemical interactions across multiple length scales, which are challenging to investigate experimentally. In silico models provide an effective framework to explore these mechanisms. This study developed an integrated multiscale modeling framework for articular cartilage. It combined finite element (FE) models at tissue and cellular scales with an intracellular gene/protein regulatory network. The network incorporated key chondrocyte mechanotransduction and inflammatory pathways. It was implemented using a semi-quantitative formalism, capturing the directional and qualitative interplay between mechanical and inflammatory stimuli on chondrocyte biology, rather than quantitatively predicting absolute gene expression levels. A Hill's function was applied to link cellular forces from the FE model to a mechanical loading input to the regulatory network. Hill's function constants were calibrated through a genetic algorithm by matching simulated and experimental gene expressions of COL-II and ADAMTS5 in cartilage explants under 20% cyclic compression. As a validation step, model simulations were performed at 10% cyclic compression of cartilage explants. Predicted sGAG loss matched the trend of experimental data. COL-II and ACAN were overestimated and ADAMTS5 was underestimated compared with experimental data. These discrepancies are consistent with the semi-quantitative nature of the model and are attributed to the simplified representation of inflammation-dominated catabolic pathways at low mechanical loads in the current framework. Simulated chondrocyte responses at different locations revealed spatial heterogeneity in chondrocyte activity. Overall, the multiscale modeling workflow developed in this study represents a first step towards a powerful platform for mechanistically deciphering the complex interplay of mechanics and inflammation in articular cartilage across multiple length scales. |
| Τύπος εγγράφου: | journal article http://purl.org/coar/resource_type/c_6501 article peer reviewed |
| Γλώσσα: | English |
| Relation: | https://api.elsevier.com/content/article/PII:S001048252600301X?httpAccept=text/xml; urn:issn:0010-4825; urn:issn:1879-0534 |
| DOI: | 10.1016/j.compbiomed.2026.111737 |
| Σύνδεσμος πρόσβασης: | https://orbi.uliege.be/handle/2268/346988 |
| Rights: | open access http://purl.org/coar/access_right/c_abf2 info:eu-repo/semantics/openAccess |
| Αριθμός Καταχώρησης: | edsorb.346988 |
| Βάση Δεδομένων: | ORBi |
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