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Green Fluorescent Protein : Applications and Protocols

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Τίτλος: Green Fluorescent Protein : Applications and Protocols
Περιγραφή: Green fluorescent protein (GFP), a critically important marker protein, allows direct visualization in live samples without the aid of additional sample preparation. In Green Fluorescent Protein: Applications and Protocols, Barry W. Hicks and a team of well-practiced experimentalists demonstrate the power and the versatility of this marker with a cutting-edge collection of readily reproducible GFP techniques. These include methods for detecting and imaging GFP (FRET, BRET, and whole-body imaging), for using GFP to create and select for transgenic organisms, for its use as a biosensor, and for studying viruses. Each method is described in step-by-step detail to ensure robust results and can be used in applications ranging from agriculture and botany to human gene therapy. Extensive notes review the materials required, explain the scientific basis of each step and possible sources of error, and provide tips for overcoming problems. Highly practical and state-of-the-art, Green Fluorescent Protein: Applications and Protocols makes available to all biological and biomedical experimentalists a full array of the powerful new GFP techniques.
Συγγραφείς: Hicks, Barry W.
Resource Type: eBook.
Θέματα: Green fluorescent protein, Green fluorescent protein--Laboratory manuals
Categories: SCIENCE / Life Sciences / Molecular Biology, SCIENCE / Life Sciences / Cell Biology
Βάση Δεδομένων: eBook Index
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  Availability: 0
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An: 201371
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PubType: eBook
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  Data: Green fluorescent protein (GFP), a critically important marker protein, allows direct visualization in live samples without the aid of additional sample preparation. In Green Fluorescent Protein: Applications and Protocols, Barry W. Hicks and a team of well-practiced experimentalists demonstrate the power and the versatility of this marker with a cutting-edge collection of readily reproducible GFP techniques. These include methods for detecting and imaging GFP (FRET, BRET, and whole-body imaging), for using GFP to create and select for transgenic organisms, for its use as a biosensor, and for studying viruses. Each method is described in step-by-step detail to ensure robust results and can be used in applications ranging from agriculture and botany to human gene therapy. Extensive notes review the materials required, explain the scientific basis of each step and possible sources of error, and provide tips for overcoming problems. Highly practical and state-of-the-art, Green Fluorescent Protein: Applications and Protocols makes available to all biological and biomedical experimentalists a full array of the powerful new GFP techniques.
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      – Code: 572.8
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      – Code: eng
        Text: English
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      – SubjectFull: Green fluorescent protein
        Type: general
      – SubjectFull: Green fluorescent protein--Laboratory manuals
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      – TitleFull: Green Fluorescent Protein : Applications and Protocols
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            – D: 01
              M: 01
              Type: published
              Y: 2002
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              M: 02
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              Value: 9781592592807
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              Value: 00183
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