Academic Journal
Decline in pneumococcal vaccine serotype carriage, multiple-serotype carriage, and carriage density in Nepalese children after PCV10 introduction: a pre-post comparison study
| Τίτλος: | Decline in pneumococcal vaccine serotype carriage, multiple-serotype carriage, and carriage density in Nepalese children after PCV10 introduction: a pre-post comparison study |
|---|---|
| Συγγραφείς: | Kandasamy, R, Gurung, M, Shrestha, S, Gautam, MC, Kelly, S, Thorson, S, Ansari, I, Gould, K, Hinds, J, Kelly, DF, Murdoch, DR, Pollard, AJ |
| Στοιχεία εκδότη: | Elsevier |
| Έτος έκδοσης: | 2024 |
| Συλλογή: | Oxford University Research Archive (ORA) |
| Περιγραφή: | Background: Carriage studies are an efficient means for assessing pneumococcal conjugate vaccine effect in settings where pneumococcal disease surveillance programmes are not well established. In this study the effect of 10-valent pneumococcal conjugate vaccine (PCV10) introduction on pneumococcal carriage and density among Nepalese children using a bacterial microarray and qPCR was examined. Methods: PCV10 was introduced into the Nepalese infant immunisation schedule in August 2015. Nasopharyngeal swabs were collected from healthy Nepalese children in Kathmandu between April 2014 and December 2021. Samples were plated on blood agar, incubated overnight, and DNA extracted from plate sweeps. Pneumococcal serotyping was done using the Senti-SPv1.5 microarray (BUGS Bioscience, UK). DNA was extracted from swab media and qPCR performed for pneumococcal autolysin (lytA). Results: A significant decline in prevalence of PCV10 serotypes was observed when comparing pre-PCV10 with post-PCV10 collection periods (36.5 %, 454/1244 vs 10.3 %, 243/2353, p < 0.0001). Multiple-serotype carriage was also observed to significantly decline when comparing pre-PCV10 with post-PCV10 periods (31.4 %, 390/ 1244 vs 22.2 %, 522/2353, p < 0.0001). Additionally, a significant decline in median neumococcal density was observed when comparing pre-PCV10 with post-PCV10 periods (3.3 vs 3.25 log10 GE/ml, p = 0.0196). Conclusions: PCV10 introduction was associated with reduced, prevalence of all PCV10 serotypes, multiple serotype carriage, and pneumococcal carriage density. |
| Τύπος εγγράφου: | article in journal/newspaper |
| Γλώσσα: | English |
| Relation: | https://doi.org/10.1016/j.vaccine.2024.05.018 |
| DOI: | 10.1016/j.vaccine.2024.05.018 |
| Διαθεσιμότητα: | https://doi.org/10.1016/j.vaccine.2024.05.018 https://ora.ox.ac.uk/objects/uuid:2b104c4a-9444-4303-9c5d-e99b9995eee1 |
| Rights: | info:eu-repo/semantics/openAccess ; CC Attribution (CC BY) |
| Αριθμός Καταχώρησης: | edsbas.FAB043FE |
| Βάση Δεδομένων: | BASE |
| FullText | Text: Availability: 0 CustomLinks: – Url: https://doi.org/10.1016/j.vaccine.2024.05.018# Name: EDS - BASE (ns324271) Category: fullText Text: View record from BASE |
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| Items | – Name: Title Label: Title Group: Ti Data: Decline in pneumococcal vaccine serotype carriage, multiple-serotype carriage, and carriage density in Nepalese children after PCV10 introduction: a pre-post comparison study – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Kandasamy%2C+R%22">Kandasamy, R</searchLink><br /><searchLink fieldCode="AR" term="%22Gurung%2C+M%22">Gurung, M</searchLink><br /><searchLink fieldCode="AR" term="%22Shrestha%2C+S%22">Shrestha, S</searchLink><br /><searchLink fieldCode="AR" term="%22Gautam%2C+MC%22">Gautam, MC</searchLink><br /><searchLink fieldCode="AR" term="%22Kelly%2C+S%22">Kelly, S</searchLink><br /><searchLink fieldCode="AR" term="%22Thorson%2C+S%22">Thorson, S</searchLink><br /><searchLink fieldCode="AR" term="%22Ansari%2C+I%22">Ansari, I</searchLink><br /><searchLink fieldCode="AR" term="%22Gould%2C+K%22">Gould, K</searchLink><br /><searchLink fieldCode="AR" term="%22Hinds%2C+J%22">Hinds, J</searchLink><br /><searchLink fieldCode="AR" term="%22Kelly%2C+DF%22">Kelly, DF</searchLink><br /><searchLink fieldCode="AR" term="%22Murdoch%2C+DR%22">Murdoch, DR</searchLink><br /><searchLink fieldCode="AR" term="%22Pollard%2C+AJ%22">Pollard, AJ</searchLink> – Name: Publisher Label: Publisher Information Group: PubInfo Data: Elsevier – Name: DatePubCY Label: Publication Year Group: Date Data: 2024 – Name: Subset Label: Collection Group: HoldingsInfo Data: Oxford University Research Archive (ORA) – Name: Abstract Label: Description Group: Ab Data: Background: Carriage studies are an efficient means for assessing pneumococcal conjugate vaccine effect in settings where pneumococcal disease surveillance programmes are not well established. In this study the effect of 10-valent pneumococcal conjugate vaccine (PCV10) introduction on pneumococcal carriage and density among Nepalese children using a bacterial microarray and qPCR was examined. Methods: PCV10 was introduced into the Nepalese infant immunisation schedule in August 2015. Nasopharyngeal swabs were collected from healthy Nepalese children in Kathmandu between April 2014 and December 2021. Samples were plated on blood agar, incubated overnight, and DNA extracted from plate sweeps. Pneumococcal serotyping was done using the Senti-SPv1.5 microarray (BUGS Bioscience, UK). DNA was extracted from swab media and qPCR performed for pneumococcal autolysin (lytA). Results: A significant decline in prevalence of PCV10 serotypes was observed when comparing pre-PCV10 with post-PCV10 collection periods (36.5 %, 454/1244 vs 10.3 %, 243/2353, p < 0.0001). Multiple-serotype carriage was also observed to significantly decline when comparing pre-PCV10 with post-PCV10 periods (31.4 %, 390/ 1244 vs 22.2 %, 522/2353, p < 0.0001). Additionally, a significant decline in median neumococcal density was observed when comparing pre-PCV10 with post-PCV10 periods (3.3 vs 3.25 log10 GE/ml, p = 0.0196). Conclusions: PCV10 introduction was associated with reduced, prevalence of all PCV10 serotypes, multiple serotype carriage, and pneumococcal carriage density. – Name: TypeDocument Label: Document Type Group: TypDoc Data: article in journal/newspaper – Name: Language Label: Language Group: Lang Data: English – Name: NoteTitleSource Label: Relation Group: SrcInfo Data: https://doi.org/10.1016/j.vaccine.2024.05.018 – Name: DOI Label: DOI Group: ID Data: 10.1016/j.vaccine.2024.05.018 – Name: URL Label: Availability Group: URL Data: https://doi.org/10.1016/j.vaccine.2024.05.018<br />https://ora.ox.ac.uk/objects/uuid:2b104c4a-9444-4303-9c5d-e99b9995eee1 – Name: Copyright Label: Rights Group: Cpyrght Data: info:eu-repo/semantics/openAccess ; CC Attribution (CC BY) – Name: AN Label: Accession Number Group: ID Data: edsbas.FAB043FE |
| PLink | https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edsbas&AN=edsbas.FAB043FE |
| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1016/j.vaccine.2024.05.018 Languages: – Text: English Titles: – TitleFull: Decline in pneumococcal vaccine serotype carriage, multiple-serotype carriage, and carriage density in Nepalese children after PCV10 introduction: a pre-post comparison study Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Kandasamy, R – PersonEntity: Name: NameFull: Gurung, M – PersonEntity: Name: NameFull: Shrestha, S – PersonEntity: Name: NameFull: Gautam, MC – PersonEntity: Name: NameFull: Kelly, S – PersonEntity: Name: NameFull: Thorson, S – PersonEntity: Name: NameFull: Ansari, I – PersonEntity: Name: NameFull: Gould, K – PersonEntity: Name: NameFull: Hinds, J – PersonEntity: Name: NameFull: Kelly, DF – PersonEntity: Name: NameFull: Murdoch, DR – PersonEntity: Name: NameFull: Pollard, AJ IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 01 Type: published Y: 2024 Identifiers: – Type: issn-locals Value: edsbas – Type: issn-locals Value: edsbas.oa |
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