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An antigen microarray protocol for COVID-19 serological analysis.

Λεπτομέρειες βιβλιογραφικής εγγραφής
Τίτλος: An antigen microarray protocol for COVID-19 serological analysis.
Συγγραφείς: LONGWORTH, Joseph, DITTMAR, Gunnar
Πηγή: STAR Protocols, 2 (3), 100815 (2021-09-17)
Στοιχεία εκδότη: Cell Press
Έτος έκδοσης: 2021
Συλλογή: University of Luxembourg: ORBilu - Open Repository and Bibliography
Θεματικοί όροι: Antibody, Bioinformatics, Health Sciences, High Throughput Screening, Immunology, Proteomics, Antibodies, Viral, Antigens, Spike Glycoprotein, Coronavirus, Viral/blood, Viral/immunology, COVID-19/blood, COVID-19/immunology, COVID-19/virology, Humans, Microarray Analysis/methods, SARS-CoV-2/immunology, Coronavirus/blood, Coronavirus/immunology, COVID-19, Microarray Analysis, SARS-CoV-2, Biochemistry, Genetics and Molecular Biology (all), Neuroscience (all), Immunology and Microbiology (all), Medicine (all), General Immunology and Microbiology
Περιγραφή: peer reviewed ; The emergence of the coronavirus disease 2019 pandemic increased the interest in analysis of immunoglobulin responses. ELISA and lateral flow assays are widely used but are restricted by a single response value to an antigen or antigen pool. Here, we describe antigen microarrays, an alternative allowing simultaneous assessment of multiple interactions between antigens and the immunoglobulin content of patient sera. The technique requires minimal reagents and sample input and can be adapted to a wide variety of potential antigenic targets of interest.
Τύπος εγγράφου: article in journal/newspaper
Γλώσσα: English
ISSN: 2666-1667
Relation: https://api.elsevier.com/content/article/PII:S2666166721005219?httpAccept=text/xml; urn:issn:2666-1667; https://orbilu.uni.lu/handle/10993/58213; info:hdl:10993/58213; info:pmid:34462737; wos:001048876800005
DOI: 10.1016/j.xpro.2021.100815
Διαθεσιμότητα: https://orbilu.uni.lu/handle/10993/58213
https://orbilu.uni.lu/bitstream/10993/58213/1/1-s2.0-S2666166721005219-main.pdf
https://doi.org/10.1016/j.xpro.2021.100815
Rights: open access ; http://purl.org/coar/access_right/c_abf2 ; info:eu-repo/semantics/openAccess
Αριθμός Καταχώρησης: edsbas.DF81755F
Βάση Δεδομένων: BASE
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  Data: peer reviewed ; The emergence of the coronavirus disease 2019 pandemic increased the interest in analysis of immunoglobulin responses. ELISA and lateral flow assays are widely used but are restricted by a single response value to an antigen or antigen pool. Here, we describe antigen microarrays, an alternative allowing simultaneous assessment of multiple interactions between antigens and the immunoglobulin content of patient sera. The technique requires minimal reagents and sample input and can be adapted to a wide variety of potential antigenic targets of interest.
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