Academic Journal
α-Synuclein plastic antibody applied to monitor monomeric structures and discriminate aggregated forms in human CSF
| Τίτλος: | α-Synuclein plastic antibody applied to monitor monomeric structures and discriminate aggregated forms in human CSF |
|---|---|
| Συγγραφείς: | da Silva, Inês S, Cardoso, Ana R, Reimer, Lasse, König, Annekatrin, van Riesen, Christoph, Outeiro, Tiago Fleming, Jensen, Poul Henning, Sales, M Goreti F |
| Πηγή: | da Silva, I S, Cardoso, A R, Reimer, L, König, A, van Riesen, C, Outeiro, T F, Jensen, P H & Sales, M G F 2025, 'α-Synuclein plastic antibody applied to monitor monomeric structures and discriminate aggregated forms in human CSF', Biosensors and Bioelectronics, vol. 268, 116880, pp. 116880. https://doi.org/10.1016/j.bios.2024.116880 |
| Έτος έκδοσης: | 2025 |
| Συλλογή: | Aarhus University: Research |
| Θεματικοί όροι: | Antibodies, Immobilized/chemistry, Antibodies/chemistry, Biosensing Techniques/methods, Dielectric Spectroscopy/methods, Electrochemical Techniques/methods, Electrodes, Humans, Limit of Detection, Methylene Blue/chemistry, Parkinson Disease/cerebrospinal fluid, Protein Aggregates, alpha-Synuclein/cerebrospinal fluid, Parkinson's disease, Plastic antibody, Carbon screen-printed electrodes, Electrochemical biosensor, Alpha-synuclein |
| Περιγραφή: | Aggregation of alpha-synuclein (aSyn) occurs in presynaptic neurons and constitutes a key factor for the progression of Parkinson's disease, emphasising the urgency of early detection to support effective treatment. Unfortunately, a reliable, sensitive and cost-effective diagnostic tool has so far been lacking. Thus, this work presents a novel biosensor for detecting aSyn using plastic antibodies coupled to electrochemical detection. This biosensor was designed for portability and compatibility with point-of-care devices and exploits the electropolymerization of methylene blue (MB) together with aSyn on the carbon working electrode of screen-printed electrodes (SPEs). By electrochemical impedance spectroscopy (EIS) measurements, the sensor showed exceptional analytical performance in detecting aSyn monomers in human CSF samples. It showed a linear trend of response from 1 fM to 10 pM with an impressively low limit of detection of 69 aM. Selectivity tests confirmed the predominant response to aSyn monomers, a less intense response to oligomers and insensitivity to fibrils. Overall, this plastic antibody-based electrochemical sensor represents a significant breakthrough as it is the first of its kind to accurately, sensitively and selectively detect aSyn monomers with a partial response to oligomers. Its simplicity and reproducibility promise to contribute to the early and effective diagnosis of Parkinson's disease. |
| Τύπος εγγράφου: | article in journal/newspaper |
| Περιγραφή αρχείου: | application/pdf |
| Γλώσσα: | English |
| ISSN: | 0956-5663 1873-4235 |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/39509995; info:eu-repo/semantics/altIdentifier/pissn/0956-5663; info:eu-repo/semantics/altIdentifier/eissn/1873-4235 |
| DOI: | 10.1016/j.bios.2024.116880 |
| Διαθεσιμότητα: | https://pure.au.dk/portal/en/publications/418edb7e-8104-42d0-8641-7b2d531ae8cd https://doi.org/10.1016/j.bios.2024.116880 https://pure.au.dk/ws/files/471792266/1-s2.0-S095656632400887X-main.pdf https://www.scopus.com/pages/publications/85208105636 |
| Rights: | info:eu-repo/semantics/openAccess ; http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| Αριθμός Καταχώρησης: | edsbas.D8C6326 |
| Βάση Δεδομένων: | BASE |
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