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Effect of whey-based culture supernatant of Lactobacillus acidophilus (johnsonii) La1 on Helicobacter pylori infection in humans

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Title: Effect of whey-based culture supernatant of Lactobacillus acidophilus (johnsonii) La1 on Helicobacter pylori infection in humans
Authors: Michetti, P., Dorta, G., Wiesel, P. H., Brassart, D., Verdu, E., Herranz, M., Felley, C., Porta, N., Rouvet, M., Blum, A. L., Corthesy-Theulaz, I.
Publication Year: 1999
Collection: Université de Lausanne (UNIL): Serval - Serveur académique lausannois
Subject Terms: Adolescent Adult Aged Antibodies, Bacterial/analysis Bacterial Adhesion Breath Tests Colony Count, Microbial Double-Blind Method Female Follow-Up Studies Gastric Mucosa/drug effects/metabolism/microbiology Gastritis/drug therapy/metabolism/*microbiology HT29 Cells/microbiology Helicobacter Infections/drug therapy/metabolism/*microbiology Helicobacter pylori/enzymology/*growth & development/immunology Humans Lactic Acid/metabolism Lactobacillus acidophilus/*physiology Male Middle Aged Omeprazole/therapeutic use Treatment Outcome Urease/metabolism
Description: BACKGROUND: Specific strains of Lactobacillus acidophilus are known to inhibit intestinal cell adhesion and invasion by enterovirulent bacteria. As L. acidophilus can survive transiently in the human stomach, it may downregulate Helicobacter pylori infection. METHODS: The ability of L. acidophilus (johnsonii) La1 supernatant to interfere with H. pylori bacterial growth, urease activity, and adhesion to epithelial cells was tested in vitro. Its effect on H. pylori infection in volunteers was monitored in a randomized, double-blind, controlled clinical trial, using a drinkable, whey-based, La1 culture supernatant. H. pylori infected volunteers were treated 14 days with 50 ml of La1 supernatant four times a day combined with either omeprazole 20 mg four times a day or with placebo. Infection was assessed by breath test, endoscopy, and biopsy sampling, performed at inclusion, immediately at the end of the treatment (breath test only), and 4 weeks after the end of the treatment. RESULTS: La1 supernatant inhibited H. pylori growth in vitro, regardless of previous binding of H. pylori to epithelial cells. In 20 subjects (8 females, 12 males, mean age 33.1 years) a marked decrease in breath test values was observed immediately after treatment with La1 supernatant, both in the omeprazole and in the placebo group (median 12.3 vs. 28.8 and 9.4 vs. 20.4, respectively; p < 0.03). In both treatment groups, breath test values remained low 6 weeks after treatment (omeprazole treated 19.2, placebo treated 8. 3; p < 0.03 vs. pretreatment), but the persistence of H. pylori infection was confirmed in gastric biopsies. CONCLUSION: La1 culture supernatant shown to be effective in vitro has a partial, acid-independent long-term suppressive effect on H. pylori in humans.
Document Type: article in journal/newspaper
Language: unknown
ISSN: 0012-2823
Relation: Digestion; https://iris.unil.ch/handle/iris/149829; serval:BIB_7BBF5CBD116C; 000080402600003
DOI: 10.1159/000007660
Availability: https://iris.unil.ch/handle/iris/149829
https://doi.org/10.1159/000007660
Accession Number: edsbas.CE578484
Database: BASE
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  Data: Effect of whey-based culture supernatant of Lactobacillus acidophilus (johnsonii) La1 on Helicobacter pylori infection in humans
– Name: Author
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  Data: &lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Michetti%2C+P%2E%22&quot;&gt;Michetti, P.&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Dorta%2C+G%2E%22&quot;&gt;Dorta, G.&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Wiesel%2C+P%2E+H%2E%22&quot;&gt;Wiesel, P. H.&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Brassart%2C+D%2E%22&quot;&gt;Brassart, D.&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Verdu%2C+E%2E%22&quot;&gt;Verdu, E.&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Herranz%2C+M%2E%22&quot;&gt;Herranz, M.&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Felley%2C+C%2E%22&quot;&gt;Felley, C.&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Porta%2C+N%2E%22&quot;&gt;Porta, N.&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Rouvet%2C+M%2E%22&quot;&gt;Rouvet, M.&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Blum%2C+A%2E+L%2E%22&quot;&gt;Blum, A. L.&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Corthesy-Theulaz%2C+I%2E%22&quot;&gt;Corthesy-Theulaz, I.&lt;/searchLink&gt;
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  Data: 1999
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  Data: Universit&#233; de Lausanne (UNIL): Serval - Serveur acad&#233;mique lausannois
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  Data: &lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Adolescent+Adult+Aged+Antibodies%22&quot;&gt;Adolescent Adult Aged Antibodies&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Bacterial%2Fanalysis+Bacterial+Adhesion+Breath+Tests+Colony+Count%22&quot;&gt;Bacterial/analysis Bacterial Adhesion Breath Tests Colony Count&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Microbial+Double-Blind+Method+Female+Follow-Up+Studies+Gastric+Mucosa%2Fdrug+effects%2Fmetabolism%2Fmicrobiology+Gastritis%2Fdrug+therapy%2Fmetabolism%2F*microbiology+HT29+Cells%2Fmicrobiology+Helicobacter+Infections%2Fdrug+therapy%2Fmetabolism%2F*microbiology+Helicobacter+pylori%2Fenzymology%2F*growth+%26+development%2Fimmunology+Humans+Lactic+Acid%2Fmetabolism+Lactobacillus+acidophilus%2F*physiology+Male+Middle+Aged+Omeprazole%2Ftherapeutic+use+Treatment+Outcome+Urease%2Fmetabolism%22&quot;&gt;Microbial Double-Blind Method Female Follow-Up Studies Gastric Mucosa/drug effects/metabolism/microbiology Gastritis/drug therapy/metabolism/*microbiology HT29 Cells/microbiology Helicobacter Infections/drug therapy/metabolism/*microbiology Helicobacter pylori/enzymology/*growth &amp; development/immunology Humans Lactic Acid/metabolism Lactobacillus acidophilus/*physiology Male Middle Aged Omeprazole/therapeutic use Treatment Outcome Urease/metabolism&lt;/searchLink&gt;
– Name: Abstract
  Label: Description
  Group: Ab
  Data: BACKGROUND: Specific strains of Lactobacillus acidophilus are known to inhibit intestinal cell adhesion and invasion by enterovirulent bacteria. As L. acidophilus can survive transiently in the human stomach, it may downregulate Helicobacter pylori infection. METHODS: The ability of L. acidophilus (johnsonii) La1 supernatant to interfere with H. pylori bacterial growth, urease activity, and adhesion to epithelial cells was tested in vitro. Its effect on H. pylori infection in volunteers was monitored in a randomized, double-blind, controlled clinical trial, using a drinkable, whey-based, La1 culture supernatant. H. pylori infected volunteers were treated 14 days with 50 ml of La1 supernatant four times a day combined with either omeprazole 20 mg four times a day or with placebo. Infection was assessed by breath test, endoscopy, and biopsy sampling, performed at inclusion, immediately at the end of the treatment (breath test only), and 4 weeks after the end of the treatment. RESULTS: La1 supernatant inhibited H. pylori growth in vitro, regardless of previous binding of H. pylori to epithelial cells. In 20 subjects (8 females, 12 males, mean age 33.1 years) a marked decrease in breath test values was observed immediately after treatment with La1 supernatant, both in the omeprazole and in the placebo group (median 12.3 vs. 28.8 and 9.4 vs. 20.4, respectively; p &lt; 0.03). In both treatment groups, breath test values remained low 6 weeks after treatment (omeprazole treated 19.2, placebo treated 8. 3; p &lt; 0.03 vs. pretreatment), but the persistence of H. pylori infection was confirmed in gastric biopsies. CONCLUSION: La1 culture supernatant shown to be effective in vitro has a partial, acid-independent long-term suppressive effect on H. pylori in humans.
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  Data: 10.1159/000007660
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        Value: 10.1159/000007660
    Languages:
      – Text: unknown
    Subjects:
      – SubjectFull: Adolescent Adult Aged Antibodies
        Type: general
      – SubjectFull: Bacterial/analysis Bacterial Adhesion Breath Tests Colony Count
        Type: general
      – SubjectFull: Microbial Double-Blind Method Female Follow-Up Studies Gastric Mucosa/drug effects/metabolism/microbiology Gastritis/drug therapy/metabolism/*microbiology HT29 Cells/microbiology Helicobacter Infections/drug therapy/metabolism/*microbiology Helicobacter pylori/enzymology/*growth & development/immunology Humans Lactic Acid/metabolism Lactobacillus acidophilus/*physiology Male Middle Aged Omeprazole/therapeutic use Treatment Outcome Urease/metabolism
        Type: general
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      – TitleFull: Effect of whey-based culture supernatant of Lactobacillus acidophilus (johnsonii) La1 on Helicobacter pylori infection in humans
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            NameFull: Michetti, P.
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            NameFull: Dorta, G.
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          Dates:
            – D: 01
              M: 01
              Type: published
              Y: 1999
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