Academic Journal
Safety and efficacy of E coli enterotoxin adjuvant for urease-based rectal immunization against Helicobacter pylori
| Τίτλος: | Safety and efficacy of E coli enterotoxin adjuvant for urease-based rectal immunization against Helicobacter pylori |
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| Συγγραφείς: | Sougioultzis, S., Lee, C. K., Alsahli, M., Banerjee, S., Cadoz, M., Schrader, R., Guy, B., Bedford, P., Monath, T. P., Kelly, C. P., Michetti, P. |
| Έτος έκδοσης: | 2002 |
| Συλλογή: | Université de Lausanne (UNIL): Serval - Serveur académique lausannois |
| Θεματικοί όροι: | Adjuvants, Immunologic/*administration & dosage/adverse effects Administration, Rectal Antibodies, Bacterial/blood Enterotoxins/*administration & dosage/adverse effects Enzyme-Linked Immunosorbent Assay Escherichia coli/chemistry Escherichia coli Vaccines/administration & dosage/adverse effects/*immunology Helicobacter Infections/*prevention & control Helicobacter pylori/*enzymology Humans Immunization Immunoglobulin A/blood Immunoglobulin G/blood Safety Urease/*administration & dosage/immunology Vaccination |
| Περιγραφή: | Low dose E. coli heat-labile enterotoxin (LT), delivered orally or enterically, has been used as an adjuvant for Helicobacter pylori (H. pylori) urease in healthy adults. In this study we aim to test the safety and adjuvant efficacy of LT delivered rectally together with recombinant H. pylori urease. Eighteen healthy adults without present or past H. pylori infection were enrolled in a double blind, randomized, ascending dose study to receive either urease (60 mg), or urease (60 mg) + LT (5 or 25 microg). The immunization preparation was administered per rectum on days 0, 14 and 28. Serum, stool and saliva anti-urease and anti-LT IgG and IgA antibodies (Abs) were measured and urease-specific and LT-specific antigen secreting cells (ASCs) were counted in peripheral blood at baseline and 7 (ASC counts) or 14 days (antibody levels) after each dosing. Peripheral blood lymphoproliferation assays were also performed at baseline and at the end of the study.Rectally delivered urease and LT were well tolerated. Among the 12 subjects assigned to urease+LT, 2 (16.7%) developed anti-urease IgG Abs, 1 (8.3%) developed anti-urease IgA Abs, and 3 (25%) showed urease-specific IgA(+) ASCs. Immune responses to LT were more vigorous, especially in subjects exposed to 5 microg LT. In the urease+ 5 microg LT group, anti-LT IgG and IgA Abs developed in 60 and 80% of the subjects, respectively, while LT-specific IgG(+) and IgA(+) ASCs were detected in all subjects. The magnitude of the anti-LT response was much higher than the response to urease. No IgA anti-urease or anti-LT Abs were detected in stool or saliva and lymphocyte proliferative responses to urease were unsatisfactory. In conclusion, rectal delivery of 5 microg LT is safe and induces vigorous systemic anti-LT immune responses. Further studies are needed to determine if LT can be an effective adjuvant for rectally delivered antigens. |
| Τύπος εγγράφου: | article in journal/newspaper |
| Γλώσσα: | unknown |
| ISBN: | 978-0-00-179723-9 0-00-179723-9 |
| ISSN: | 0264-410X |
| Relation: | Vaccine; https://iris.unil.ch/handle/iris/153418; serval:BIB_A0396846A97D; 000179723900007 |
| DOI: | 10.1016/S0264-410X(02)00467-X |
| Διαθεσιμότητα: | https://iris.unil.ch/handle/iris/153418 https://doi.org/10.1016/S0264-410X(02)00467-X |
| Αριθμός Καταχώρησης: | edsbas.C9BAD761 |
| Βάση Δεδομένων: | BASE |
| ISBN: | 9780001797239 0001797239 |
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| ISSN: | 0264410X |
| DOI: | 10.1016/S0264-410X(02)00467-X |