Academic Journal
The mechanical response of vinculin
| Τίτλος: | The mechanical response of vinculin |
|---|---|
| Συγγραφείς: | Liu, Xuyao, Liu, Jingzhun, Wang, Yinan, Yao, Mingxi, Baker, Karen B, Klapholz, Benjamin, Brown, Nicholas H, Goult, Benjamin T, Yan, Jie |
| Στοιχεία εκδότη: | American Association for the Advancement of Science (AAAS) |
| Έτος έκδοσης: | 2025 |
| Συλλογή: | The University of Liverpool Repository |
| Περιγραφή: | Vinculin is a mechanosensitive adaptor that links actin to cell-matrix and cell-cell adhesions. Known as a mechanoeffector, it is recruited to adhesion sites under force via mechanotransducers talin and α-catenin. Here, we examine vinculin’s mechanical properties to assess its role as a mechanotransducer. We find that at physiological loading rates, vinculin domains unfold at forces of 5 to 15 pN and refold rapidly when forces drop to 1 pN. This behavior is reminiscent of force-dependent switches in talin and α-catenin, suggesting vinculin domains also function as molecular switches. Unfolding induces large extension changes up to 150 nm in steps of 20 to 30 nm. These findings reveal that vinculin exhibits a previously unrecognized mechanical response, with dynamic folding/ unfolding under force acting as a buffering mechanism. Given its role as a scaffold for many proteins, this mechanosensitive behavior supports a model where vinculin functions directly as a mechanotransducer, recruiting binding partners in a force-dependent manner. |
| Τύπος εγγράφου: | article in journal/newspaper |
| Περιγραφή αρχείου: | application/pdf |
| Γλώσσα: | English |
| ISSN: | 2375-2548 |
| Relation: | https://livrepository.liverpool.ac.uk/3195140/1/2025_Liu_SciAdv.pdf; Liu, Xuyao, Liu, Jingzhun, Wang, Yinan, Yao, Mingxi, Baker, Karen B, Klapholz, Benjamin, Brown, Nicholas H, Goult, Benjamin T orcid:0000-0002-3438-2807 and Yan, Jie (2025) The mechanical response of vinculin SCIENCE ADVANCES, 11 (44). pp. 1-10. ISSN 2375-2548, 2375-2548 |
| DOI: | 10.1126/sciadv.ady6949 |
| Διαθεσιμότητα: | https://livrepository.liverpool.ac.uk/3195140/ https://doi.org/10.1126/sciadv.ady6949 https://livrepository.liverpool.ac.uk/3195140/1/2025_Liu_SciAdv.pdf |
| Αριθμός Καταχώρησης: | edsbas.C9845EAE |
| Βάση Δεδομένων: | BASE |
| ISSN: | 23752548 |
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| DOI: | 10.1126/sciadv.ady6949 |