Academic Journal

Presence of multiple 'Helicobacter heilmannii' strains in an individual suffering from ulcers and in his two cats

Bibliographic Details
Title: Presence of multiple 'Helicobacter heilmannii' strains in an individual suffering from ulcers and in his two cats
Authors: Dieterich, C., Wiesel, P., Neiger, R., Blum, A., Corthesy-Theulaz, I.
Publication Year: 1998
Collection: Université de Lausanne (UNIL): Serval - Serveur académique lausannois
Subject Terms: Adult Animals Base Sequence Cats/*microbiology Helicobacter/*classification/enzymology/isolation & purification Helicobacter Infections/enzymology/*microbiology/veterinary Humans Male Molecular Sequence Data Polymorphism, Restriction Fragment Length Repetitive Sequences, Nucleic Acid Restriction Mapping Sequence Alignment Sequence Homology, Nucleic Acid Stomach Ulcer/*microbiology Urease/analysis Zoonoses/*microbiology
Description: Circumstantial evidence suggests that "Helicobacter heilmannii" infection is an example of zoonosis. The presence of "H. heilmannii" strains in a human subject with acute gastric erosions, in his two cats, and in two unrelated cats was analyzed, and the genetic relatedness of the human and feline strains was assessed. A 580-bp, PCR-amplified sequence of "H. heilmannii" urease B gene (ureB) obtained from biopsies from the human subject and his two cats was restricted with AluI and cloned for sequencing. Analysis of the restriction fragment length polymorphism of the ureB-amplified product suggested the presence of different individual "H. heilmannii" strains in the cats and of three distinct strains in the human subject. One of the "H. heilmannii" ureB sequences amplified from the human subject's biopsies was identical to that derived from one of his cats. The degree of similarity between the other "H. heilmannii" human and feline nucleotide sequences was higher than 97%. Most of the base substitutions were conservative. We conclude that human and animal "H. heilmannii" strains are closely related and that humans can be infected by more than one "H. heilmannii" strain, as has been observed for Helicobacter pylori.
Document Type: article in journal/newspaper
Language: unknown
ISSN: 0095-1137
Relation: Journal of Clinical Microbiology; https://iris.unil.ch/handle/iris/98891; serval:BIB_4669CB8F8BC7; 000073177900038; 9574707
Availability: https://iris.unil.ch/handle/iris/98891
Accession Number: edsbas.C27C95DD
Database: BASE
Description
ISSN:00951137