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HLA-DQ Typing Kits in Diagnosis and Screening for Celiac Disease

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Title: HLA-DQ Typing Kits in Diagnosis and Screening for Celiac Disease
Authors: Rouvroye, Maxine D, van Zijtveld, Sander, Bonnet, Petra, Spierings, Eric, Bontkes, Hetty J, CDL Patiëntenzorg MI, Infection & Immunity
Publication Year: 2019
Subject Terms: HLA-DQ, HLA-DQ7, celiac disease, diagnosis, screening, Celiac Disease/diagnosis, Humans, Risk Factors, Genetic Predisposition to Disease/genetics, Genotype, Male, Genetic Markers, Gene Frequency/genetics, Genetic Testing/methods, Reagent Kits, Diagnostic, Alleles, Female, Histocompatibility Testing/methods, HLA-DQ Antigens/genetics, Taverne, Genetics(clinical), Journal Article, Comparative Study
Description: Aim: Celiac disease (CD) is strongly associated with HLA-DQ2.2, HLA-DQ2.5, and HLA-DQ8. Up to 99.7% of all CD patients are positive for either one or two of these genetic markers, demonstrating a high negative predictive value. This has led to the development of diagnostic kits that, instead of providing a full HLA-DQ typing, detect only these three HLA-DQ types. Our aim was to compare three different kits for their performance, utilization, and costs. Because 0.4-3.6% of all CD patients test positive for HLA-DQ7 and negative for the aforementioned types, information provided by the kits regarding DQ7 alpha and beta chains was evaluated as well. Materials and Methods: Fifty DNA samples previously typed with the SSCP method were analyzed using three commercial kits. Results and Discussion: All kits report hetero- or homozygosity for HLA-DQ2.5. The XeliGen kit directly detects HLA-DQ7, but is relatively expensive. The MLPA kit is the least expensive in terms of reagents and may indirectly detect HLA-DQ7. The CeliaSCAN kit is easy to use and provides indirect information about HLA-DQ7.5. Conclusion: All kits correctly identify the CD risk genes. The resources of the laboratory and the intended use should determine the preference for any of the HLA-DQ typing kits herein described.
Document Type: article in journal/newspaper
File Description: text/plain
Language: English
ISSN: 1945-0265
Relation: https://dspace.library.uu.nl/handle/1874/391365
Availability: https://dspace.library.uu.nl/handle/1874/391365
Rights: info:eu-repo/semantics/OpenAccess
Accession Number: edsbas.B596B49E
Database: BASE
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  Data: HLA-DQ Typing Kits in Diagnosis and Screening for Celiac Disease
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  Data: <searchLink fieldCode="AR" term="%22Rouvroye%2C+Maxine+D%22">Rouvroye, Maxine D</searchLink><br /><searchLink fieldCode="AR" term="%22van+Zijtveld%2C+Sander%22">van Zijtveld, Sander</searchLink><br /><searchLink fieldCode="AR" term="%22Bonnet%2C+Petra%22">Bonnet, Petra</searchLink><br /><searchLink fieldCode="AR" term="%22Spierings%2C+Eric%22">Spierings, Eric</searchLink><br /><searchLink fieldCode="AR" term="%22Bontkes%2C+Hetty+J%22">Bontkes, Hetty J</searchLink><br /><searchLink fieldCode="AR" term="%22CDL+Patiëntenzorg+MI%22">CDL Patiëntenzorg MI</searchLink><br /><searchLink fieldCode="AR" term="%22Infection+%26+Immunity%22">Infection & Immunity</searchLink>
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  Data: 2019
– Name: Subject
  Label: Subject Terms
  Group: Su
  Data: <searchLink fieldCode="DE" term="%22HLA-DQ%22">HLA-DQ</searchLink><br /><searchLink fieldCode="DE" term="%22HLA-DQ7%22">HLA-DQ7</searchLink><br /><searchLink fieldCode="DE" term="%22celiac+disease%22">celiac disease</searchLink><br /><searchLink fieldCode="DE" term="%22diagnosis%22">diagnosis</searchLink><br /><searchLink fieldCode="DE" term="%22screening%22">screening</searchLink><br /><searchLink fieldCode="DE" term="%22Celiac+Disease%2Fdiagnosis%22">Celiac Disease/diagnosis</searchLink><br /><searchLink fieldCode="DE" term="%22Humans%22">Humans</searchLink><br /><searchLink fieldCode="DE" term="%22Risk+Factors%22">Risk Factors</searchLink><br /><searchLink fieldCode="DE" term="%22Genetic+Predisposition+to+Disease%2Fgenetics%22">Genetic Predisposition to Disease/genetics</searchLink><br /><searchLink fieldCode="DE" term="%22Genotype%22">Genotype</searchLink><br /><searchLink fieldCode="DE" term="%22Male%22">Male</searchLink><br /><searchLink fieldCode="DE" term="%22Genetic+Markers%22">Genetic Markers</searchLink><br /><searchLink fieldCode="DE" term="%22Gene+Frequency%2Fgenetics%22">Gene Frequency/genetics</searchLink><br /><searchLink fieldCode="DE" term="%22Genetic+Testing%2Fmethods%22">Genetic Testing/methods</searchLink><br /><searchLink fieldCode="DE" term="%22Reagent+Kits%22">Reagent Kits</searchLink><br /><searchLink fieldCode="DE" term="%22Diagnostic%22">Diagnostic</searchLink><br /><searchLink fieldCode="DE" term="%22Alleles%22">Alleles</searchLink><br /><searchLink fieldCode="DE" term="%22Female%22">Female</searchLink><br /><searchLink fieldCode="DE" term="%22Histocompatibility+Testing%2Fmethods%22">Histocompatibility Testing/methods</searchLink><br /><searchLink fieldCode="DE" term="%22HLA-DQ+Antigens%2Fgenetics%22">HLA-DQ Antigens/genetics</searchLink><br /><searchLink fieldCode="DE" term="%22Taverne%22">Taverne</searchLink><br /><searchLink fieldCode="DE" term="%22Genetics%28clinical%29%22">Genetics(clinical)</searchLink><br /><searchLink fieldCode="DE" term="%22Journal+Article%22">Journal Article</searchLink><br /><searchLink fieldCode="DE" term="%22Comparative+Study%22">Comparative Study</searchLink>
– Name: Abstract
  Label: Description
  Group: Ab
  Data: Aim: Celiac disease (CD) is strongly associated with HLA-DQ2.2, HLA-DQ2.5, and HLA-DQ8. Up to 99.7% of all CD patients are positive for either one or two of these genetic markers, demonstrating a high negative predictive value. This has led to the development of diagnostic kits that, instead of providing a full HLA-DQ typing, detect only these three HLA-DQ types. Our aim was to compare three different kits for their performance, utilization, and costs. Because 0.4-3.6% of all CD patients test positive for HLA-DQ7 and negative for the aforementioned types, information provided by the kits regarding DQ7 alpha and beta chains was evaluated as well. Materials and Methods: Fifty DNA samples previously typed with the SSCP method were analyzed using three commercial kits. Results and Discussion: All kits report hetero- or homozygosity for HLA-DQ2.5. The XeliGen kit directly detects HLA-DQ7, but is relatively expensive. The MLPA kit is the least expensive in terms of reagents and may indirectly detect HLA-DQ7. The CeliaSCAN kit is easy to use and provides indirect information about HLA-DQ7.5. Conclusion: All kits correctly identify the CD risk genes. The resources of the laboratory and the intended use should determine the preference for any of the HLA-DQ typing kits herein described.
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  Data: 1945-0265
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  Data: https://dspace.library.uu.nl/handle/1874/391365
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  Data: edsbas.B596B49E
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RecordInfo BibRecord:
  BibEntity:
    Languages:
      – Text: English
    Subjects:
      – SubjectFull: HLA-DQ
        Type: general
      – SubjectFull: HLA-DQ7
        Type: general
      – SubjectFull: celiac disease
        Type: general
      – SubjectFull: diagnosis
        Type: general
      – SubjectFull: screening
        Type: general
      – SubjectFull: Celiac Disease/diagnosis
        Type: general
      – SubjectFull: Humans
        Type: general
      – SubjectFull: Risk Factors
        Type: general
      – SubjectFull: Genetic Predisposition to Disease/genetics
        Type: general
      – SubjectFull: Genotype
        Type: general
      – SubjectFull: Male
        Type: general
      – SubjectFull: Genetic Markers
        Type: general
      – SubjectFull: Gene Frequency/genetics
        Type: general
      – SubjectFull: Genetic Testing/methods
        Type: general
      – SubjectFull: Reagent Kits
        Type: general
      – SubjectFull: Diagnostic
        Type: general
      – SubjectFull: Alleles
        Type: general
      – SubjectFull: Female
        Type: general
      – SubjectFull: Histocompatibility Testing/methods
        Type: general
      – SubjectFull: HLA-DQ Antigens/genetics
        Type: general
      – SubjectFull: Taverne
        Type: general
      – SubjectFull: Genetics(clinical)
        Type: general
      – SubjectFull: Journal Article
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      – SubjectFull: Comparative Study
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      – TitleFull: HLA-DQ Typing Kits in Diagnosis and Screening for Celiac Disease
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            NameFull: Bontkes, Hetty J
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