Bibliographic Details
| Title: |
Sorting and pooling strategy: a novel tool to map a virus proteome for CD8 T-cell epitopes |
| Authors: |
Bernhard Ay, Mathias Streitz, Prisca Boisguerin, Andreas Schlosser, Carsten C. Mahrenholz, Sebastian D. Schuck, Florian Kern, Rudolf Volkmer |
| Publication Year: |
2007 |
| Collection: |
University of Sussex (US): Figshare |
| Subject Terms: |
Amino Acid Sequence, Biopolymers/chemistry, CD8-Positive T-Lymphocytes/*immunology, Cytomegalovirus/*genetics/*immunology, Epitope Mapping/*methods, Epitopes/*chemistry, Flow Cytometry, Genome, Viral, Humans, Oligopeptides/chemistry, Peptide Mapping, Proteome, Proteomics/*methods |
| Description: |
Human cytomegalovirus (CMV) is a major cause of morbidity in immunocompromised individuals. However, no efficient vaccine has been developed to date. Identification of T-cell target proteins and epitopes is crucial not only for developing a successful immunization strategy, but also for new approaches using adoptive transfer of antigen-specific T-cells. The CMV genome has more than 200 open reading frames potentially coding for as many proteins. Here, we describe a robust, fast, and simple SPOT synthesis strategy, which allowed us to micro-synthesize every possible CD8 T-cell epitope in the entire potential CMV proteome. So far, 9069 of these peptides have been tested in an ex vivo T-cell stimulation assay. As well as confirming a number of previously known epitopes, we identified several new ones. |
| Document Type: |
article in journal/newspaper |
| Language: |
unknown |
| Relation: |
10779/uos.23389010.v1 |
| Availability: |
https://figshare.com/articles/journal_contribution/Sorting_and_pooling_strategy_a_novel_tool_to_map_a_virus_proteome_for_CD8_T-cell_epitopes/23389010 |
| Rights: |
Copyright not evaluated |
| Accession Number: |
edsbas.B07207A5 |
| Database: |
BASE |