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Sorting and pooling strategy: a novel tool to map a virus proteome for CD8 T-cell epitopes

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Τίτλος: Sorting and pooling strategy: a novel tool to map a virus proteome for CD8 T-cell epitopes
Συγγραφείς: Bernhard Ay, Mathias Streitz, Prisca Boisguerin, Andreas Schlosser, Carsten C. Mahrenholz, Sebastian D. Schuck, Florian Kern, Rudolf Volkmer
Έτος έκδοσης: 2007
Συλλογή: University of Sussex (US): Figshare
Θεματικοί όροι: Amino Acid Sequence, Biopolymers/chemistry, CD8-Positive T-Lymphocytes/*immunology, Cytomegalovirus/*genetics/*immunology, Epitope Mapping/*methods, Epitopes/*chemistry, Flow Cytometry, Genome, Viral, Humans, Oligopeptides/chemistry, Peptide Mapping, Proteome, Proteomics/*methods
Περιγραφή: Human cytomegalovirus (CMV) is a major cause of morbidity in immunocompromised individuals. However, no efficient vaccine has been developed to date. Identification of T-cell target proteins and epitopes is crucial not only for developing a successful immunization strategy, but also for new approaches using adoptive transfer of antigen-specific T-cells. The CMV genome has more than 200 open reading frames potentially coding for as many proteins. Here, we describe a robust, fast, and simple SPOT synthesis strategy, which allowed us to micro-synthesize every possible CD8 T-cell epitope in the entire potential CMV proteome. So far, 9069 of these peptides have been tested in an ex vivo T-cell stimulation assay. As well as confirming a number of previously known epitopes, we identified several new ones.
Τύπος εγγράφου: article in journal/newspaper
Γλώσσα: unknown
Relation: 10779/uos.23389010.v1
Διαθεσιμότητα: https://figshare.com/articles/journal_contribution/Sorting_and_pooling_strategy_a_novel_tool_to_map_a_virus_proteome_for_CD8_T-cell_epitopes/23389010
Rights: Copyright not evaluated
Αριθμός Καταχώρησης: edsbas.B07207A5
Βάση Δεδομένων: BASE
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  – Url: https://figshare.com/articles/journal_contribution/Sorting_and_pooling_strategy_a_novel_tool_to_map_a_virus_proteome_for_CD8_T-cell_epitopes/23389010#
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PubType: Academic Journal
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  Data: Sorting and pooling strategy: a novel tool to map a virus proteome for CD8 T-cell epitopes
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  Data: <searchLink fieldCode="AR" term="%22Bernhard+Ay%22">Bernhard Ay</searchLink><br /><searchLink fieldCode="AR" term="%22Mathias+Streitz%22">Mathias Streitz</searchLink><br /><searchLink fieldCode="AR" term="%22Prisca+Boisguerin%22">Prisca Boisguerin</searchLink><br /><searchLink fieldCode="AR" term="%22Andreas+Schlosser%22">Andreas Schlosser</searchLink><br /><searchLink fieldCode="AR" term="%22Carsten+C%2E+Mahrenholz%22">Carsten C. Mahrenholz</searchLink><br /><searchLink fieldCode="AR" term="%22Sebastian+D%2E+Schuck%22">Sebastian D. Schuck</searchLink><br /><searchLink fieldCode="AR" term="%22Florian+Kern%22">Florian Kern</searchLink><br /><searchLink fieldCode="AR" term="%22Rudolf+Volkmer%22">Rudolf Volkmer</searchLink>
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  Data: 2007
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  Data: University of Sussex (US): Figshare
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  Data: <searchLink fieldCode="DE" term="%22Amino+Acid+Sequence%22">Amino Acid Sequence</searchLink><br /><searchLink fieldCode="DE" term="%22Biopolymers%2Fchemistry%22">Biopolymers/chemistry</searchLink><br /><searchLink fieldCode="DE" term="%22CD8-Positive+T-Lymphocytes%2F*immunology%22">CD8-Positive T-Lymphocytes/*immunology</searchLink><br /><searchLink fieldCode="DE" term="%22Cytomegalovirus%2F*genetics%2F*immunology%22">Cytomegalovirus/*genetics/*immunology</searchLink><br /><searchLink fieldCode="DE" term="%22Epitope+Mapping%2F*methods%22">Epitope Mapping/*methods</searchLink><br /><searchLink fieldCode="DE" term="%22Epitopes%2F*chemistry%22">Epitopes/*chemistry</searchLink><br /><searchLink fieldCode="DE" term="%22Flow+Cytometry%22">Flow Cytometry</searchLink><br /><searchLink fieldCode="DE" term="%22Genome%22">Genome</searchLink><br /><searchLink fieldCode="DE" term="%22Viral%22">Viral</searchLink><br /><searchLink fieldCode="DE" term="%22Humans%22">Humans</searchLink><br /><searchLink fieldCode="DE" term="%22Oligopeptides%2Fchemistry%22">Oligopeptides/chemistry</searchLink><br /><searchLink fieldCode="DE" term="%22Peptide+Mapping%22">Peptide Mapping</searchLink><br /><searchLink fieldCode="DE" term="%22Proteome%22">Proteome</searchLink><br /><searchLink fieldCode="DE" term="%22Proteomics%2F*methods%22">Proteomics/*methods</searchLink>
– Name: Abstract
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  Data: Human cytomegalovirus (CMV) is a major cause of morbidity in immunocompromised individuals. However, no efficient vaccine has been developed to date. Identification of T-cell target proteins and epitopes is crucial not only for developing a successful immunization strategy, but also for new approaches using adoptive transfer of antigen-specific T-cells. The CMV genome has more than 200 open reading frames potentially coding for as many proteins. Here, we describe a robust, fast, and simple SPOT synthesis strategy, which allowed us to micro-synthesize every possible CD8 T-cell epitope in the entire potential CMV proteome. So far, 9069 of these peptides have been tested in an ex vivo T-cell stimulation assay. As well as confirming a number of previously known epitopes, we identified several new ones.
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  Label: Relation
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  Data: 10779/uos.23389010.v1
– Name: URL
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  Data: https://figshare.com/articles/journal_contribution/Sorting_and_pooling_strategy_a_novel_tool_to_map_a_virus_proteome_for_CD8_T-cell_epitopes/23389010
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  Data: edsbas.B07207A5
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RecordInfo BibRecord:
  BibEntity:
    Languages:
      – Text: unknown
    Subjects:
      – SubjectFull: Amino Acid Sequence
        Type: general
      – SubjectFull: Biopolymers/chemistry
        Type: general
      – SubjectFull: CD8-Positive T-Lymphocytes/*immunology
        Type: general
      – SubjectFull: Cytomegalovirus/*genetics/*immunology
        Type: general
      – SubjectFull: Epitope Mapping/*methods
        Type: general
      – SubjectFull: Epitopes/*chemistry
        Type: general
      – SubjectFull: Flow Cytometry
        Type: general
      – SubjectFull: Genome
        Type: general
      – SubjectFull: Viral
        Type: general
      – SubjectFull: Humans
        Type: general
      – SubjectFull: Oligopeptides/chemistry
        Type: general
      – SubjectFull: Peptide Mapping
        Type: general
      – SubjectFull: Proteome
        Type: general
      – SubjectFull: Proteomics/*methods
        Type: general
    Titles:
      – TitleFull: Sorting and pooling strategy: a novel tool to map a virus proteome for CD8 T-cell epitopes
        Type: main
  BibRelationships:
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      – PersonEntity:
          Name:
            NameFull: Bernhard Ay
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            NameFull: Mathias Streitz
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            NameFull: Prisca Boisguerin
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            NameFull: Andreas Schlosser
      – PersonEntity:
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            NameFull: Carsten C. Mahrenholz
      – PersonEntity:
          Name:
            NameFull: Sebastian D. Schuck
      – PersonEntity:
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            NameFull: Florian Kern
      – PersonEntity:
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            NameFull: Rudolf Volkmer
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          Dates:
            – D: 01
              M: 01
              Type: published
              Y: 2007
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