Academic Journal
Programmable Nanoscale Motion via Molecular Patterning on DNA Origami
| Title: | Programmable Nanoscale Motion via Molecular Patterning on DNA Origami |
|---|---|
| Authors: | Paffen, Lars, Engelbert van Bevervoorde, Maurik, Rodriguez-Abetxuko, Andoni, Abdelmohsen, Loai, van der Hofstad, Remco, van Hest, Jan C.M., Patiño Padial, Tania |
| Source: | Paffen, L, Engelbert van Bevervoorde, M, Rodriguez-Abetxuko, A, Abdelmohsen, L, van der Hofstad, R, van Hest, J C M & Patiño Padial, T 2026, 'Programmable Nanoscale Motion via Molecular Patterning on DNA Origami', Angewandte Chemie - International Edition, vol. 65, no. 8, e23921. https://doi.org/10.1002/anie.202523921 |
| Publication Year: | 2026 |
| Subject Terms: | DNA origami, Enzyme kinetics, Nanomotors, Single-particle tracking, Nanotubes/chemistry, Motion, DNA/chemistry, Urease/metabolism, Nanotechnology |
| Description: | Asymmetry in enzymatically driven nanomotor design, both structural and functional, is widely considered essential for propulsion. However, the interplay between particle geometry, enzyme distribution, and catalytic loading remains poorly defined, largely due to limited control over enzyme positioning that hinders quantitative analysis. Using DNA origami nanorods, we achieve precise spatial placement of urease enzymes with independently tunable coverage and asymmetry. Single-particle tracking reveals that motility arises not solely from enzyme number or spatial arrangement but from a balance between catalytic loading and geometric anisotropy. Unexpectedly, maximal propulsion occurs at ∼25% urease end-coverage, significantly below the conventional 50% end-coverage, where half of the available binding positions on one structural half of the origami are occupied. Boundary Element Method simulations incorporating identical spatial parameters reproduce these findings, confirming that programmable enzyme patterning dictates diffusiophoretic propulsion. These results provide a quantitative framework linking topology, catalytic activity, and motion, revealing that optimal motility does not coincide with maximal asymmetry and advancing the rational design of enzyme-powered DNA nanomotors. |
| Document Type: | article in journal/newspaper |
| File Description: | application/pdf |
| Language: | English |
| ISSN: | 1433-7851 1521-3773 |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/41546160; info:eu-repo/semantics/altIdentifier/pissn/1433-7851; info:eu-repo/semantics/altIdentifier/eissn/1521-3773 |
| DOI: | 10.1002/anie.202523921 |
| Availability: | https://research.tue.nl/en/publications/e2ba0223-bef4-438b-ad8a-3948f0457c0d https://doi.org/10.1002/anie.202523921 https://pure.tue.nl/ws/files/381187458/Angew_Chem_Int_Ed_-_2026_-_Paffen_-_Programmable_Nanoscale_Motion_via_Molecular_Patterning_on_DNA_Origami.pdf https://www.scopus.com/pages/publications/105027655065 |
| Rights: | info:eu-repo/semantics/openAccess ; http://creativecommons.org/licenses/by/4.0/ |
| Accession Number: | edsbas.97781BFB |
| Database: | BASE |
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| Items | – Name: Title Label: Title Group: Ti Data: Programmable Nanoscale Motion via Molecular Patterning on DNA Origami – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Paffen%2C+Lars%22">Paffen, Lars</searchLink><br /><searchLink fieldCode="AR" term="%22Engelbert+van+Bevervoorde%2C+Maurik%22">Engelbert van Bevervoorde, Maurik</searchLink><br /><searchLink fieldCode="AR" term="%22Rodriguez-Abetxuko%2C+Andoni%22">Rodriguez-Abetxuko, Andoni</searchLink><br /><searchLink fieldCode="AR" term="%22Abdelmohsen%2C+Loai%22">Abdelmohsen, Loai</searchLink><br /><searchLink fieldCode="AR" term="%22van+der+Hofstad%2C+Remco%22">van der Hofstad, Remco</searchLink><br /><searchLink fieldCode="AR" term="%22van+Hest%2C+Jan+C%2EM%2E%22">van Hest, Jan C.M.</searchLink><br /><searchLink fieldCode="AR" term="%22Patiño+Padial%2C+Tania%22">Patiño Padial, Tania</searchLink> – Name: TitleSource Label: Source Group: Src Data: Paffen, L, Engelbert van Bevervoorde, M, Rodriguez-Abetxuko, A, Abdelmohsen, L, van der Hofstad, R, van Hest, J C M & Patiño Padial, T 2026, 'Programmable Nanoscale Motion via Molecular Patterning on DNA Origami', Angewandte Chemie - International Edition, vol. 65, no. 8, e23921. https://doi.org/10.1002/anie.202523921 – Name: DatePubCY Label: Publication Year Group: Date Data: 2026 – Name: Subject Label: Subject Terms Group: Su Data: <searchLink fieldCode="DE" term="%22DNA+origami%22">DNA origami</searchLink><br /><searchLink fieldCode="DE" term="%22Enzyme+kinetics%22">Enzyme kinetics</searchLink><br /><searchLink fieldCode="DE" term="%22Nanomotors%22">Nanomotors</searchLink><br /><searchLink fieldCode="DE" term="%22Single-particle+tracking%22">Single-particle tracking</searchLink><br /><searchLink fieldCode="DE" term="%22Nanotubes%2Fchemistry%22">Nanotubes/chemistry</searchLink><br /><searchLink fieldCode="DE" term="%22Motion%22">Motion</searchLink><br /><searchLink fieldCode="DE" term="%22DNA%2Fchemistry%22">DNA/chemistry</searchLink><br /><searchLink fieldCode="DE" term="%22Urease%2Fmetabolism%22">Urease/metabolism</searchLink><br /><searchLink fieldCode="DE" term="%22Nanotechnology%22">Nanotechnology</searchLink> – Name: Abstract Label: Description Group: Ab Data: Asymmetry in enzymatically driven nanomotor design, both structural and functional, is widely considered essential for propulsion. However, the interplay between particle geometry, enzyme distribution, and catalytic loading remains poorly defined, largely due to limited control over enzyme positioning that hinders quantitative analysis. Using DNA origami nanorods, we achieve precise spatial placement of urease enzymes with independently tunable coverage and asymmetry. Single-particle tracking reveals that motility arises not solely from enzyme number or spatial arrangement but from a balance between catalytic loading and geometric anisotropy. Unexpectedly, maximal propulsion occurs at ∼25% urease end-coverage, significantly below the conventional 50% end-coverage, where half of the available binding positions on one structural half of the origami are occupied. Boundary Element Method simulations incorporating identical spatial parameters reproduce these findings, confirming that programmable enzyme patterning dictates diffusiophoretic propulsion. These results provide a quantitative framework linking topology, catalytic activity, and motion, revealing that optimal motility does not coincide with maximal asymmetry and advancing the rational design of enzyme-powered DNA nanomotors. – Name: TypeDocument Label: Document Type Group: TypDoc Data: article in journal/newspaper – Name: Format Label: File Description Group: SrcInfo Data: application/pdf – Name: Language Label: Language Group: Lang Data: English – Name: ISSN Label: ISSN Group: ISSN Data: 1433-7851<br />1521-3773 – Name: NoteTitleSource Label: Relation Group: SrcInfo Data: info:eu-repo/semantics/altIdentifier/pmid/41546160; info:eu-repo/semantics/altIdentifier/pissn/1433-7851; info:eu-repo/semantics/altIdentifier/eissn/1521-3773 – Name: DOI Label: DOI Group: ID Data: 10.1002/anie.202523921 – Name: URL Label: Availability Group: URL Data: https://research.tue.nl/en/publications/e2ba0223-bef4-438b-ad8a-3948f0457c0d<br />https://doi.org/10.1002/anie.202523921<br />https://pure.tue.nl/ws/files/381187458/Angew_Chem_Int_Ed_-_2026_-_Paffen_-_Programmable_Nanoscale_Motion_via_Molecular_Patterning_on_DNA_Origami.pdf<br />https://www.scopus.com/pages/publications/105027655065 – Name: Copyright Label: Rights Group: Cpyrght Data: info:eu-repo/semantics/openAccess ; http://creativecommons.org/licenses/by/4.0/ – Name: AN Label: Accession Number Group: ID Data: edsbas.97781BFB |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1002/anie.202523921 Languages: – Text: English Subjects: – SubjectFull: DNA origami Type: general – SubjectFull: Enzyme kinetics Type: general – SubjectFull: Nanomotors Type: general – SubjectFull: Single-particle tracking Type: general – SubjectFull: Nanotubes/chemistry Type: general – SubjectFull: Motion Type: general – SubjectFull: DNA/chemistry Type: general – SubjectFull: Urease/metabolism Type: general – SubjectFull: Nanotechnology Type: general Titles: – TitleFull: Programmable Nanoscale Motion via Molecular Patterning on DNA Origami Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Paffen, Lars – PersonEntity: Name: NameFull: Engelbert van Bevervoorde, Maurik – PersonEntity: Name: NameFull: Rodriguez-Abetxuko, Andoni – PersonEntity: Name: NameFull: Abdelmohsen, Loai – PersonEntity: Name: NameFull: van der Hofstad, Remco – PersonEntity: Name: NameFull: van Hest, Jan C.M. – PersonEntity: Name: NameFull: Patiño Padial, Tania IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 01 Type: published Y: 2026 Identifiers: – Type: issn-print Value: 14337851 – Type: issn-print Value: 15213773 – Type: issn-locals Value: edsbas – Type: issn-locals Value: edsbas.oa Titles: – TitleFull: Paffen, L, Engelbert van Bevervoorde, M, Rodriguez-Abetxuko, A, Abdelmohsen, L, van der Hofstad, R, van Hest, J C M & Patiño Padial, T 2026, 'Programmable Nanoscale Motion via Molecular Patterning on DNA Origami', Angewandte Chemie - International Edition, vol. 65, no. 8, e23921. https://doi.org/10.1002/anie.202523921 Type: main |
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