Other/Unknown Material
Development of NASBA-primer search software for designing forensic saliva tandem repeat markers for mucin and amylase ; Development of nucleic acid sequence based amplification primer search software for designing forensic saliva tandem repeat markers for mucin and amylase
| Τίτλος: | Development of NASBA-primer search software for designing forensic saliva tandem repeat markers for mucin and amylase ; Development of nucleic acid sequence based amplification primer search software for designing forensic saliva tandem repeat markers for mucin and amylase |
|---|---|
| Συγγραφείς: | Ara, Andleeb. |
| Συνεισφορές: | McKillip, John L. |
| Πηγή: | CardinalScholar 1.0 |
| Έτος έκδοσης: | 2009 |
| Συλλογή: | Ball State University: Cardinal Scholar |
| Θεματικοί όροι: | Nucleotide sequence -- Computer programs, Mucins -- Analysis -- Computer programs, Amylases -- Analysis -- Computer programs, Forensic genetics -- Computer programs |
| Περιγραφή: | Access to thesis permanently restricted to Ball State community only ; Nucleic Acid Sequence Based Amplification (NASBA) is a powerful in vitro technique for amplification. NASBA is routinely used in many fields of microbiology, including food microbiology, and most recently in the identification of forensic body secretions (saliva, tears, sweat, semen, vaginal secretions). NASBA has many advantages over the traditional Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) including speed, high sample throughput and increased sensitivity. Proper selection of the sequence of importance and the designe of NASBA primers precisely for that sequence are the two most critical steps for any NASBA assay. Proper designe of NASBA primers includes important considerations such as product (amplicon) length, the addition of a T7 RNA polymerase promoter sequence at the 5’end of one primer, and a 3’AT rich region. Primer designing is, therefore, laborious and error-prone. Currently, no such software is available that facilitates primer designing for NASBA. In this study Java-based software for designing NASBA primers was developed which will enable rapid and specific NASBA primer designing for gene expression studies. The designed program focused on scripting minimum Java coding lines to reduce the time and storage space. Two codes were scripted for this software, a pseudo-code (for Java Program Developers) and Byte-code (for Windows operating system). Our results showed that Java is an efficient tool in searching sequences of interest within a gene (or mRNA), allowing for NASBA primers to be designed more quickly and effortlessly. The program has maximum memory storage capacity and allows the users to retrieve old data with reference to date or time. To test the practicality of the newly developed program, gene sequences of salivary mucin and amylase were examined to facilitate extraction of novel RNA tandem repeat element NASBA markers for human saliva forensic identification. Tandem repeats of non-coding portion with in ... |
| Τύπος εγγράφου: | other/unknown material |
| Περιγραφή αρχείου: | vii, 66 p. : digital, PDF file, ill. (some col.).; application/pdf |
| Γλώσσα: | unknown |
| Relation: | http://liblink.bsu.edu/catkey/1493906; http://cardinalscholar.bsu.edu/handle/20.500.14291/193466 |
| Διαθεσιμότητα: | http://cardinalscholar.bsu.edu/handle/20.500.14291/193466 https://hdl.handle.net/20.500.14291/193466 http://liblink.bsu.edu/catkey/1493906 |
| Αριθμός Καταχώρησης: | edsbas.917412E3 |
| Βάση Δεδομένων: | BASE |
| FullText | Text: Availability: 0 CustomLinks: – Url: http://cardinalscholar.bsu.edu/handle/20.500.14291/193466# Name: EDS - BASE (ns324271) Category: fullText Text: View record from BASE |
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| Header | DbId: edsbas DbLabel: BASE An: edsbas.917412E3 RelevancyScore: 692 AccessLevel: 3 PubType: Other/Unknown Material PubTypeId: unknown PreciseRelevancyScore: 691.994323730469 |
| IllustrationInfo | |
| Items | – Name: Title Label: Title Group: Ti Data: Development of NASBA-primer search software for designing forensic saliva tandem repeat markers for mucin and amylase ; Development of nucleic acid sequence based amplification primer search software for designing forensic saliva tandem repeat markers for mucin and amylase – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Ara%2C+Andleeb%2E%22">Ara, Andleeb.</searchLink> – Name: Author Label: Contributors Group: Au Data: McKillip, John L. – Name: TitleSource Label: Source Group: Src Data: CardinalScholar 1.0 – Name: DatePubCY Label: Publication Year Group: Date Data: 2009 – Name: Subset Label: Collection Group: HoldingsInfo Data: Ball State University: Cardinal Scholar – Name: Subject Label: Subject Terms Group: Su Data: <searchLink fieldCode="DE" term="%22Nucleotide+sequence+--+Computer+programs%22">Nucleotide sequence -- Computer programs</searchLink><br /><searchLink fieldCode="DE" term="%22Mucins+--+Analysis+--+Computer+programs%22">Mucins -- Analysis -- Computer programs</searchLink><br /><searchLink fieldCode="DE" term="%22Amylases+--+Analysis+--+Computer+programs%22">Amylases -- Analysis -- Computer programs</searchLink><br /><searchLink fieldCode="DE" term="%22Forensic+genetics+--+Computer+programs%22">Forensic genetics -- Computer programs</searchLink> – Name: Abstract Label: Description Group: Ab Data: Access to thesis permanently restricted to Ball State community only ; Nucleic Acid Sequence Based Amplification (NASBA) is a powerful in vitro technique for amplification. NASBA is routinely used in many fields of microbiology, including food microbiology, and most recently in the identification of forensic body secretions (saliva, tears, sweat, semen, vaginal secretions). NASBA has many advantages over the traditional Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) including speed, high sample throughput and increased sensitivity. Proper selection of the sequence of importance and the designe of NASBA primers precisely for that sequence are the two most critical steps for any NASBA assay. Proper designe of NASBA primers includes important considerations such as product (amplicon) length, the addition of a T7 RNA polymerase promoter sequence at the 5’end of one primer, and a 3’AT rich region. Primer designing is, therefore, laborious and error-prone. Currently, no such software is available that facilitates primer designing for NASBA. In this study Java-based software for designing NASBA primers was developed which will enable rapid and specific NASBA primer designing for gene expression studies. The designed program focused on scripting minimum Java coding lines to reduce the time and storage space. Two codes were scripted for this software, a pseudo-code (for Java Program Developers) and Byte-code (for Windows operating system). Our results showed that Java is an efficient tool in searching sequences of interest within a gene (or mRNA), allowing for NASBA primers to be designed more quickly and effortlessly. The program has maximum memory storage capacity and allows the users to retrieve old data with reference to date or time. To test the practicality of the newly developed program, gene sequences of salivary mucin and amylase were examined to facilitate extraction of novel RNA tandem repeat element NASBA markers for human saliva forensic identification. Tandem repeats of non-coding portion with in ... – Name: TypeDocument Label: Document Type Group: TypDoc Data: other/unknown material – Name: Format Label: File Description Group: SrcInfo Data: vii, 66 p. : digital, PDF file, ill. (some col.).; application/pdf – Name: Language Label: Language Group: Lang Data: unknown – Name: NoteTitleSource Label: Relation Group: SrcInfo Data: http://liblink.bsu.edu/catkey/1493906; http://cardinalscholar.bsu.edu/handle/20.500.14291/193466 – Name: URL Label: Availability Group: URL Data: http://cardinalscholar.bsu.edu/handle/20.500.14291/193466<br />https://hdl.handle.net/20.500.14291/193466<br />http://liblink.bsu.edu/catkey/1493906 – Name: AN Label: Accession Number Group: ID Data: edsbas.917412E3 |
| PLink | https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edsbas&AN=edsbas.917412E3 |
| RecordInfo | BibRecord: BibEntity: Languages: – Text: unknown Subjects: – SubjectFull: Nucleotide sequence -- Computer programs Type: general – SubjectFull: Mucins -- Analysis -- Computer programs Type: general – SubjectFull: Amylases -- Analysis -- Computer programs Type: general – SubjectFull: Forensic genetics -- Computer programs Type: general Titles: – TitleFull: Development of NASBA-primer search software for designing forensic saliva tandem repeat markers for mucin and amylase ; Development of nucleic acid sequence based amplification primer search software for designing forensic saliva tandem repeat markers for mucin and amylase Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Ara, Andleeb. – PersonEntity: Name: NameFull: McKillip, John L. IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 01 Type: published Y: 2009 Identifiers: – Type: issn-locals Value: edsbas Titles: – TitleFull: CardinalScholar 1.0 Type: main |
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