Academic Journal
Regulatory Rewiring Drives Intraspecies Competition in Bacillus subtilis
| Τίτλος: | Regulatory Rewiring Drives Intraspecies Competition in Bacillus subtilis |
|---|---|
| Συγγραφείς: | Kalamara, Margarita, Bonsall, Alistair, Griffin, Jonathan, Carneiro, Joana, Gierlinski, Marek, Eigentler, Lukas, Stevenson, David, Wood, Amy, Porter, Michael, Dorfmueller, Helge, MacPhee, Cait E., Abbott, James, Stanley-Wall, Nicola |
| Πηγή: | Kalamara, M, Bonsall, A, Griffin, J, Carneiro, J, Gierlinski, M, Eigentler, L, Stevenson, D, Wood, A, Porter, M, Dorfmueller, H, MacPhee, C E, Abbott, J & Stanley-Wall, N 2026, 'Regulatory Rewiring Drives Intraspecies Competition in Bacillus subtilis', PLoS Genetics, vol. 22, no. 2, e1012050, pp. e1012050. https://doi.org/10.1371/journal.pgen.1012050 |
| Έτος έκδοσης: | 2026 |
| Συλλογή: | Discovery - University of Dundee Online Publications |
| Θεματικοί όροι: | Bacillus subtilis, Intraspecies competition, Quorum sensing, ComQXPA system, Experimental evolution, Regulatory rewiring, Sensor kinase mutations, /dk/atira/pure/subjectarea/asjc/1100/1105, name=Ecology, Evolution, Behavior and Systematics, /dk/atira/pure/subjectarea/asjc/1300/1312, name=Molecular Biology, /dk/atira/pure/subjectarea/asjc/1300/1311, name=Genetics, /dk/atira/pure/subjectarea/asjc/2700/2716, name=Genetics(clinical), /dk/atira/pure/subjectarea/asjc/1300/1306, name=Cancer Research |
| Περιγραφή: | Intraspecies interactions shape microbial community structure and evolution, yet the mechanisms determining competitive outcomes among closely related strains remain unclear. The soil bacterium Bacillus subtilis is a model for microbial social interactions, where quorum-sensing systems regulate cooperation and antagonism. Here, we take a multifaceted approach to dissect the role of quorum-sensing regulation in competitive fitness. Isolate NCIB 3610 carries a signal unresponsive RapP-PhrP module that alters quorum-sensing control and promotes faster growth. Modelling and mutant analysis demonstrate that the small differences in growth rate conferred by RapP-PhrP3610 are sufficient to drive competitive exclusion. The importance of quorum sensing control is further exemplified by experimental evolution of distinct wild isolates, which revealed recurrent mutations in the sensor kinase comP, which phenocopy complete comP or comA deletions and confer a growth-linked competitive advantage. Key quorum sensing mechanisms are abandoned even in structured microbial communities, where it might be expected that communal traits are favoured. Furthermore, a phylogenomic survey of 370 B. subtilis genomes identified disruptive comP mutations in ~16% of isolates. However, growth rate alone does not explain all interaction outcomes as even isogenic strains with equivalent doubling times differ in competitiveness. Transcriptomic profiling and validation experiments implicated a type VII secretion system toxin as an additional effector. These findings reveal that disruption of quorum-sensing pathways, whether naturally or through selection, provides a rapid route to competitive advantage, highlighting a fundamental trade-off between communal signalling and individual fitness in microbial populations. |
| Τύπος εγγράφου: | article in journal/newspaper |
| Περιγραφή αρχείου: | application/pdf |
| Γλώσσα: | English |
| ISSN: | 1553-7390 1553-7404 |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/41701782; info:eu-repo/semantics/altIdentifier/pissn/1553-7390; info:eu-repo/semantics/altIdentifier/eissn/1553-7404; info:eu-repo/semantics/reference/pmid/42013052 |
| DOI: | 10.1371/journal.pgen.1012050 |
| Διαθεσιμότητα: | https://discovery.dundee.ac.uk/en/publications/3320335a-65d3-40f7-86a8-2c072d8d45f2 https://doi.org/10.1371/journal.pgen.1012050 https://discovery.dundee.ac.uk/ws/files/164484665/journal.pgen.1012050.pdf https://www.scopus.com/pages/publications/105031381937 |
| Rights: | info:eu-repo/semantics/openAccess ; http://creativecommons.org/licenses/by/4.0/ |
| Αριθμός Καταχώρησης: | edsbas.8FF78D7E |
| Βάση Δεδομένων: | BASE |
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