Academic Journal
Detergent-Free Functionalization of Hybrid Vesicles with Membrane Proteins Using SMALPs
| Title: | Detergent-Free Functionalization of Hybrid Vesicles with Membrane Proteins Using SMALPs |
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| Authors: | Catania, R, Machin, J, Rappolt, M, Muench, SP, Beales, PA, Jeuken, LJC |
| Publisher Information: | American Chemical Society |
| Publication Year: | 2022 |
| Collection: | White Rose Research Online (Universities of Leeds, Sheffield & York) |
| Description: | Hybrid vesicles (HVs) that consist of mixtures of block copolymers and lipids are robust biomimetics of liposomes, providing a valuable building block in bionanotechnology, catalysis, and synthetic biology. However, functionalization of HVs with membrane proteins remains laborious and expensive, creating a significant current challenge in the field. Here, using a new approach of extraction with styrene-maleic acid (SMA), we show that a membrane protein (cytochrome bo3) directly transfers into HVs with an efficiency of 73.9 ± 13.5% without the requirement of detergent, long incubation times, or mechanical disruption. Direct transfer of membrane proteins using this approach was not possible into liposomes, suggesting that HVs are more amenable than liposomes to membrane protein incorporation from a SMA lipid particle system. Finally, we show that this transfer method is not limited to cytochrome bo3 and can also be performed with complex membrane protein mixtures. |
| Document Type: | article in journal/newspaper |
| File Description: | text |
| Language: | English |
| ISSN: | 0024-9297 |
| Relation: | https://eprints.whiterose.ac.uk/id/eprint/186081/10/acs.macromol.2c00326.pdf; Catania, R, Machin, J, Rappolt, M orcid.org/0000-0001-9942-3035 et al. (3 more authors) (2022) Detergent-Free Functionalization of Hybrid Vesicles with Membrane Proteins Using SMALPs. Macromolecules, 55 (9). pp. 3415-3422. ISSN: 0024-9297 |
| Availability: | https://eprints.whiterose.ac.uk/id/eprint/186081/ https://eprints.whiterose.ac.uk/id/eprint/186081/10/acs.macromol.2c00326.pdf |
| Rights: | cc_by_4 |
| Accession Number: | edsbas.8EA73C6F |
| Database: | BASE |
| ISSN: | 00249297 |
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