Clinical confocal laser endomicroscopy for imaging of autofluorescence signals of human brain tumors and non-tumor brain

Λεπτομέρειες βιβλιογραφικής εγγραφής
Τίτλος:	Clinical confocal laser endomicroscopy for imaging of autofluorescence signals of human brain tumors and non-tumor brain
Συγγραφείς:	Reichenbach, Marlen, Richter, Sven, Galli, Roberta, Meinhardt, Matthias, Kirsche, Katrin, Temme, Achim, Emmanouilidis, Dimitrios, Polanski, Witold, Prilop, Insa, Krex, Dietmar, Sobottka, Stephan B., Juratli, Tareq A., Eyüpoglu, Ilker Y., Uckermann, Ortrud
Πηγή:	http://lobid.org/resources/99370674181806441#!, 151(1):19.
Έτος έκδοσης:	2024
Συλλογή:	Publisso (ZB MED-Publikationsportal Lebenswissenschaften)
Θεματικοί όροι:	Female [MeSH], Brain/pathology [MeSH], Microscopy, Confocal/methods [MeSH], Brain/diagnostic imaging [MeSH], Aged [MeSH], Adult [MeSH], Humans [MeSH], In situ pathology, Autofluorescence, Middle Aged [MeSH], Glioblastoma/pathology [MeSH], Brain tumor recognition, Optical Imaging/methods [MeSH], Male [MeSH], Research, Label-free, Brain Neoplasms/diagnostic imaging [MeSH], Glioblastoma/diagnostic imaging [MeSH], Intraoperative imaging, Brain Neoplasms/pathology [MeSH]
Περιγραφή:	Abstract Purpose Analysis of autofluorescence holds promise for brain tumor delineation and diagnosis. Therefore, we investigated the potential of a commercial confocal laser scanning endomicroscopy (CLE) system for clinical imaging of brain tumors. Methods A clinical CLE system with fiber probe and 488 nm laser excitation was used to acquire images of tissue autofluorescence. Fresh samples were obtained from routine surgeries (glioblastoma n = 6, meningioma n = 6, brain metastases n = 10, pituitary adenoma n = 2, non-tumor from surgery for the treatment of pharmacoresistant epilepsy n = 2). Additionally, in situ intraoperative label-free CLE was performed in three cases. The autofluorescence images were visually inspected for feature identification and quantification. For reference, tissue cryosections were prepared and further analyzed by label-free multiphoton microscopy and HE histology. Results Label-free CLE enabled the acquisition of autofluorescence images for all cases. Autofluorescent structures were assigned to the cytoplasmic compartment of cells, elastin fibers, psammoma bodies and blood vessels by comparison to references. Sparse punctuated autofluorescence was identified in most images across all cases, while dense punctuated autofluorescence was most frequent in glioblastomas. Autofluorescent cells were observed in higher abundancies in images of non-tumor samples. Diffuse autofluorescence, fibers and round fluorescent structures were predominantly found in tumor tissues. ...
Τύπος εγγράφου:	article in journal/newspaper
Γλώσσα:	English
Relation:	https://repository.publisso.de/resource/frl:6523007; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11671560/
DOI:	10.1007/s00432-024-06052-2
Διαθεσιμότητα:	https://repository.publisso.de/resource/frl:6523007 https://doi.org/10.1007/s00432-024-06052-2 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11671560/
Rights:	https://creativecommons.org/licenses/by/4.0/
Αριθμός Καταχώρησης:	edsbas.868C5F69
Βάση Δεδομένων:	BASE

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