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Clinical confocal laser endomicroscopy for imaging of autofluorescence signals of human brain tumors and non-tumor brain

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Title: Clinical confocal laser endomicroscopy for imaging of autofluorescence signals of human brain tumors and non-tumor brain
Authors: Reichenbach, Marlen, Richter, Sven, Galli, Roberta, Meinhardt, Matthias, Kirsche, Katrin, Temme, Achim, Emmanouilidis, Dimitrios, Polanski, Witold, Prilop, Insa, Krex, Dietmar, Sobottka, Stephan B., Juratli, Tareq A., Eyüpoglu, Ilker Y., Uckermann, Ortrud
Source: http://lobid.org/resources/99370674181806441#!, 151(1):19.
Publication Year: 2024
Collection: Publisso (ZB MED-Publikationsportal Lebenswissenschaften)
Subject Terms: Female [MeSH], Brain/pathology [MeSH], Microscopy, Confocal/methods [MeSH], Brain/diagnostic imaging [MeSH], Aged [MeSH], Adult [MeSH], Humans [MeSH], In situ pathology, Autofluorescence, Middle Aged [MeSH], Glioblastoma/pathology [MeSH], Brain tumor recognition, Optical Imaging/methods [MeSH], Male [MeSH], Research, Label-free, Brain Neoplasms/diagnostic imaging [MeSH], Glioblastoma/diagnostic imaging [MeSH], Intraoperative imaging, Brain Neoplasms/pathology [MeSH]
Description: Abstract Purpose Analysis of autofluorescence holds promise for brain tumor delineation and diagnosis. Therefore, we investigated the potential of a commercial confocal laser scanning endomicroscopy (CLE) system for clinical imaging of brain tumors. Methods A clinical CLE system with fiber probe and 488 nm laser excitation was used to acquire images of tissue autofluorescence. Fresh samples were obtained from routine surgeries (glioblastoma n = 6, meningioma n = 6, brain metastases n = 10, pituitary adenoma n = 2, non-tumor from surgery for the treatment of pharmacoresistant epilepsy n = 2). Additionally, in situ intraoperative label-free CLE was performed in three cases. The autofluorescence images were visually inspected for feature identification and quantification. For reference, tissue cryosections were prepared and further analyzed by label-free multiphoton microscopy and HE histology. Results Label-free CLE enabled the acquisition of autofluorescence images for all cases. Autofluorescent structures were assigned to the cytoplasmic compartment of cells, elastin fibers, psammoma bodies and blood vessels by comparison to references. Sparse punctuated autofluorescence was identified in most images across all cases, while dense punctuated autofluorescence was most frequent in glioblastomas. Autofluorescent cells were observed in higher abundancies in images of non-tumor samples. Diffuse autofluorescence, fibers and round fluorescent structures were predominantly found in tumor tissues. ...
Document Type: article in journal/newspaper
Language: English
Relation: https://repository.publisso.de/resource/frl:6523007; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11671560/
DOI: 10.1007/s00432-024-06052-2
Availability: https://repository.publisso.de/resource/frl:6523007
https://doi.org/10.1007/s00432-024-06052-2
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11671560/
Rights: https://creativecommons.org/licenses/by/4.0/
Accession Number: edsbas.868C5F69
Database: BASE
Description
DOI:10.1007/s00432-024-06052-2