Academic Journal
How big is the time window for cell-free fetal DNA testing after pregnancy loss and which factors are associated with a successful result?
| Τίτλος: | How big is the time window for cell-free fetal DNA testing after pregnancy loss and which factors are associated with a successful result? |
|---|---|
| Συγγραφείς: | El Sammaa-Aru, Yasmin Isabella, Hartwig, Tanja Schlaikjær, Bro-Jørgensen, Maiken Hemme, Münter, Emma Juuel, Werge, Lene, Banasik, Karina, Stener Jørgensen, Finn, Ambye, Louise, Westergaard, David, Nielsen, Henriette Svarre |
| Συνεισφορές: | Kirks Foundation, BioInnovation Institute Foundation, Novo Nordisk Foundation, A.P. Møller Foundation |
| Πηγή: | Human Reproduction ; ISSN 0268-1161 1460-2350 |
| Στοιχεία εκδότη: | Oxford University Press (OUP) |
| Έτος έκδοσης: | 2026 |
| Περιγραφή: | STUDY QUESTION How fast does cell-free fetal DNA (cffDNA) decline after early pregnancy loss and which factors affect the decline? SUMMARY ANSWER After pregnancy loss cffDNA declines gradually with detectable levels persisting up to 3 days post-tissue passage correlating with β-hCG decline. WHAT IS KNOWN ALREADY Postpartum clearance of cffDNA occurs within hours, but little is known about its decline following early pregnancy loss. Initial results from the Copenhagen Pregnancy Loss (COPL) study showed slower clearance in relation to pregnancy loss with detectable levels found up to 24 h after tissue passage. STUDY DESIGN, SIZE, DURATION This prospective cohort study included 1463 women from the COPL cohort, enrolled between 12 November 2020 and 19 December 2022. Participants were divided into three groups based on sampling time: the standard group (samples collected before tissue passage), the delayed sample group (samples collected after tissue passage at maximum 24 h), and the repeated sample group (samples collected at multiple time points after medical and surgical treatment). PARTICIPANTS/MATERIALS, SETTING, METHODS Eligible women were 18 years old with a confirmed intrauterine pregnancy loss before 22 gestational weeks. Exclusion criteria included ectopic, molar, or unknown-location pregnancies and inability to consent. For the repeated sample group, additional exclusions were vaginal bleeding at diagnosis, anembryonic pregnancies, and opting for expectant management. Blood samples were analyzed for β-hCG and cffDNA, with fetal fraction measured using the sequencing-based fetal fraction (SeqFF) method. In the repeated sampling group, analyses were performed on Days 2 and 3 for surgically treated and Days 7 and 14 for medically treated. MAIN RESULTS AND THE ROLE OF CHANCE After pregnancy tissue passage, both cffDNA and β-hCG levels declined consistently over time with a corresponding increase in no-call rates. The decline in SeqFF following pregnancy loss occurred more gradually than the immediate ... |
| Τύπος εγγράφου: | article in journal/newspaper |
| Γλώσσα: | English |
| DOI: | 10.1093/humrep/deag034 |
| DOI: | 10.1093/humrep/deag034/67500819/deag034.pdf |
| Διαθεσιμότητα: | https://doi.org/10.1093/humrep/deag034 https://academic.oup.com/humrep/advance-article-pdf/doi/10.1093/humrep/deag034/67500819/deag034.pdf |
| Rights: | https://academic.oup.com/pages/standard-publication-reuse-rights |
| Αριθμός Καταχώρησης: | edsbas.7F01C93A |
| Βάση Δεδομένων: | BASE |
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