Academic Journal

Systemic inflammation increases shear stress‐induced platelet plug formation measured by the PFA‐100

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Title: Systemic inflammation increases shear stress‐induced platelet plug formation measured by the PFA‐100
Authors: Homoncik, Monika, Blann, Andrew D., Hollenstein, Ursula, Pernerstorfer, Thomas, Eichler, Hans‐Georg, Jilma, Bernd
Source: British Journal of Haematology ; volume 111, issue 4, page 1250-1252 ; ISSN 0007-1048 1365-2141
Publisher Information: Wiley
Publication Year: 2000
Collection: Wiley Online Library (Open Access Articles via Crossref)
Description: The PFA‐100 measures platelet plug formation under shear stress and is strongly dependent on von Willebrand Factor (VWF) levels in plasma. We therefore hypothesized that elevated VWF levels, possibly as a result of acute inflammation, adversely influence PFA‐100 results. Healthy volunteers received either 2 ng/kg endotoxin or placebo in a randomized controlled trial. Four hours after endotoxin (but not placebo) infusion VWF levels increased by 85%, collagen epinephrine‐induced closure time (CT) decreased by 47% and collagen ADP‐CT decreased by 38% ( P < 0·0001) respectively. In conclusion, systemic inflammation has a major impact on the results obtained by PFA‐100 and may confound interpretation of platelet function.
Document Type: article in journal/newspaper
Language: English
DOI: 10.1111/j.1365-2141.2000.02473.x
Availability: https://doi.org/10.1111/j.1365-2141.2000.02473.x
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Accession Number: edsbas.7D18F7FD
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  Data: Systemic inflammation increases shear stress‐induced platelet plug formation measured by the PFA‐100
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  Data: &lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Homoncik%2C+Monika%22&quot;&gt;Homoncik, Monika&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Blann%2C+Andrew+D%2E%22&quot;&gt;Blann, Andrew D.&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Hollenstein%2C+Ursula%22&quot;&gt;Hollenstein, Ursula&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Pernerstorfer%2C+Thomas%22&quot;&gt;Pernerstorfer, Thomas&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Eichler%2C+Hans‐Georg%22&quot;&gt;Eichler, Hans‐Georg&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Jilma%2C+Bernd%22&quot;&gt;Jilma, Bernd&lt;/searchLink&gt;
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  Data: British Journal of Haematology ; volume 111, issue 4, page 1250-1252 ; ISSN 0007-1048 1365-2141
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  Data: 2000
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  Data: The PFA‐100 measures platelet plug formation under shear stress and is strongly dependent on von Willebrand Factor (VWF) levels in plasma. We therefore hypothesized that elevated VWF levels, possibly as a result of acute inflammation, adversely influence PFA‐100 results. Healthy volunteers received either 2 ng/kg endotoxin or placebo in a randomized controlled trial. Four hours after endotoxin (but not placebo) infusion VWF levels increased by 85%, collagen epinephrine‐induced closure time (CT) decreased by 47% and collagen ADP‐CT decreased by 38% ( P &lt; 0&#183;0001) respectively. In conclusion, systemic inflammation has a major impact on the results obtained by PFA‐100 and may confound interpretation of platelet function.
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