Academic Journal
EFFECTS OF FETAL BOVINE SERUM CONCENTRATION AND VENTED FLASK CONDITIONS ON E-11 CELL GROWTH AND TILAPIA LAKE VIRUS PROPAGATION
| Title: | EFFECTS OF FETAL BOVINE SERUM CONCENTRATION AND VENTED FLASK CONDITIONS ON E-11 CELL GROWTH AND TILAPIA LAKE VIRUS PROPAGATION |
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| Authors: | Zainal Abidin, Nurhamimah, Aziz, Muhamad Afiq, Fadzillah , Nur Fadiah Wathiqah, Azmai, Mohammad Noor Amal |
| Source: | Journal of Academia; Vol. 14 No. 1 (2026): April 2026; 55-63 ; 2289-6368 |
| Publisher Information: | UiTM Press |
| Publication Year: | 2026 |
| Subject Terms: | Aquaculture, Tilapia Lake Virus (TiLV), E-11 cells, Cytopathic effect |
| Description: | Tilapia Lake Virus (TiLV) is an emerging pathogen threatening global tilapia aquaculture, causing high mortality and significant economic losses. The present study aimed to optimize E-11 cell culture conditions for improved growth and TiLV propagation under different serum and flask ventilation conditions. E-11 cells were cultured in Leibovitz’s L-15 medium supplemented with 10%, 15%, and 20% fetal bovine serum (FBS) and maintained at 25°C in a CO₂-free incubator. Cell viability was assessed over four days using the Trypan Blue exclusion assay, while TiLV propagation was evaluated in both seal-cap and vent-cap flasks through microscopic observation of cytopathic effects (CPE). Results showed that E-11 cells supplemented with 15% FBS exhibited the highest viability (85–90%) and optimal proliferation compared to cells grown in 10% or 20% FBS. Morphologically, all cultures displayed similar epithelial-like characteristics with polygonal cell shapes and intact monolayers under the microscope. No significant differences in viability were observed between open- and closed-vented flasks; however, CPE were detected only in infected E-11 cells maintained in vent-cap flasks by 10 days post-infection (dpi), while cells in seal-cap flasks remained morphologically intact. These findings suggest that a 15% FBS concentration and a vented culture environment support both healthy E-11 cell growth and effective TiLV propagation. This optimized culture system enhances the reliability of in vitro TiLV studies and supports efforts toward sustainable aquaculture and food security. |
| Document Type: | article in journal/newspaper |
| File Description: | application/pdf |
| Language: | English |
| Relation: | https://journal.uitm.edu.my/ojs/index.php/index/article/view/9648/5734; https://journal.uitm.edu.my/ojs/index.php/index/article/view/9648 |
| DOI: | 10.24191/joa.v14i1.9648 |
| Availability: | https://journal.uitm.edu.my/ojs/index.php/index/article/view/9648 https://doi.org/10.24191/joa.v14i1.9648 |
| Rights: | Copyright (c) 2026 Journal of Academia ; https://creativecommons.org/licenses/by-nc-nd/4.0 |
| Accession Number: | edsbas.7C35A2A8 |
| Database: | BASE |
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