Academic Journal
SHBG Gene Polymorphisms and Their Influence on Serum SHBG, Total and Free Testosterone Concentrations in Men
| Τίτλος: | SHBG Gene Polymorphisms and Their Influence on Serum SHBG, Total and Free Testosterone Concentrations in Men |
|---|---|
| Συγγραφείς: | Walravens, Joeri, Sleumer, Bas, Vos, Michel J., Snaterse, Gido, Narinx, Nick, Antonio, Leen, Reyns, Tim, Fiers, Tom, Kema, Ido P., Kaufman, Jean Marc, Van De Merbel, Nico C., Lapauw, Bruno |
| Πηγή: | Walravens, J, Sleumer, B, Vos, M J, Snaterse, G, Narinx, N, Antonio, L, Reyns, T, Fiers, T, Kema, I P, Kaufman, J M, Van De Merbel, N C & Lapauw, B 2025, 'SHBG Gene Polymorphisms and Their Influence on Serum SHBG, Total and Free Testosterone Concentrations in Men', Journal of Clinical Endocrinology and Metabolism, vol. 110, no. 3, pp. e641-e649. https://doi.org/10.1210/clinem/dgae280 |
| Έτος έκδοσης: | 2025 |
| Συλλογή: | University of Groningen research database |
| Θεματικοί όροι: | free testosterone, healthy men, LC-MS/MS, polymorphisms, SHBG, testosterone |
| Περιγραφή: | Context: Genetic variation in SHBG structure may affect estimates of sex steroid exposure by altering the affinity of the protein for its ligand. Consequently, free hormone calculations assuming constant binding affinity may, for certain genetic variations, lead to incorrect diagnoses if genetic variation is not taken into consideration. Objective: To investigate the effects of genetic variation in SHBG on calculated and measured serum free testosterone (T) in men. Design, setting and participants: Population-based sibling-pair study in 999 healthy men aged 25 to 45 (mean, 34.5) years. Main outcome measures: Genotyping using microarray (Illumina) for single-nucleotide polymorphism (SNPs) suggested to affect binding affinity and/or concentration of SHBG or T. SHBG concentrations were measured using immunoassay and in a subset (n = 32) by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Total T was measured using LC-MS/MS. Free T was calculated and in a subset (n = 314) measured directly using LC-MS/MS after equilibrium dialysis. Results: Allelic frequencies of analyzed SNPs ranged from 0.5% to 58.2%. Compared to wild-type, SHBG concentrations were lower in rs6258 heterozygotes (-24.7%; P <. 05) and higher in rs6259 heterozygotes, rs727428 homozygotes, and carriers of rs1799941 (+10.8 to 23.1%; all P <. 05). Total T was higher in rs727428 homozygotes and carriers of rs5934505, rs1799941and rs6259 (+3.9 to 21.4%; all P <. 05). No clear effects on measured free T were found, except for a trend toward higher values in rs6259 homozygotes, significant for calculated free T (+18.7%; P <. 05) in the larger global study population. Conclusion: In these men, analyzed SNPs were relatively prevalent and affected serum concentrations of total T and SHBG but not calculated or measured free T except for a higher trend in rs6259 homozygotes. |
| Τύπος εγγράφου: | article in journal/newspaper |
| Περιγραφή αρχείου: | application/pdf |
| Γλώσσα: | English |
| ISSN: | 0021-972X 1945-7197 |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/38652149; info:eu-repo/semantics/altIdentifier/hdl/https://hdl.handle.net/11370/28d52265-4311-4754-8ff0-6a47ac595650; info:eu-repo/semantics/altIdentifier/pissn/0021-972X; info:eu-repo/semantics/altIdentifier/eissn/1945-7197 |
| DOI: | 10.1210/clinem/dgae280 |
| Διαθεσιμότητα: | https://hdl.handle.net/11370/28d52265-4311-4754-8ff0-6a47ac595650 https://research.rug.nl/en/publications/28d52265-4311-4754-8ff0-6a47ac595650 https://doi.org/10.1210/clinem/dgae280 https://pure.rug.nl/ws/files/1316746356/dgae280.pdf https://www.scopus.com/pages/publications/85218103018 |
| Rights: | info:eu-repo/semantics/openAccess ; https://www.rug.nl/library/open-access/article-25fa-pilot-end-user-agreement |
| Αριθμός Καταχώρησης: | edsbas.7B4B8EE0 |
| Βάση Δεδομένων: | BASE |
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| Items | – Name: Title Label: Title Group: Ti Data: SHBG Gene Polymorphisms and Their Influence on Serum SHBG, Total and Free Testosterone Concentrations in Men – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Walravens%2C+Joeri%22">Walravens, Joeri</searchLink><br /><searchLink fieldCode="AR" term="%22Sleumer%2C+Bas%22">Sleumer, Bas</searchLink><br /><searchLink fieldCode="AR" term="%22Vos%2C+Michel+J%2E%22">Vos, Michel J.</searchLink><br /><searchLink fieldCode="AR" term="%22Snaterse%2C+Gido%22">Snaterse, Gido</searchLink><br /><searchLink fieldCode="AR" term="%22Narinx%2C+Nick%22">Narinx, Nick</searchLink><br /><searchLink fieldCode="AR" term="%22Antonio%2C+Leen%22">Antonio, Leen</searchLink><br /><searchLink fieldCode="AR" term="%22Reyns%2C+Tim%22">Reyns, Tim</searchLink><br /><searchLink fieldCode="AR" term="%22Fiers%2C+Tom%22">Fiers, Tom</searchLink><br /><searchLink fieldCode="AR" term="%22Kema%2C+Ido+P%2E%22">Kema, Ido P.</searchLink><br /><searchLink fieldCode="AR" term="%22Kaufman%2C+Jean+Marc%22">Kaufman, Jean Marc</searchLink><br /><searchLink fieldCode="AR" term="%22Van+De+Merbel%2C+Nico+C%2E%22">Van De Merbel, Nico C.</searchLink><br /><searchLink fieldCode="AR" term="%22Lapauw%2C+Bruno%22">Lapauw, Bruno</searchLink> – Name: TitleSource Label: Source Group: Src Data: Walravens, J, Sleumer, B, Vos, M J, Snaterse, G, Narinx, N, Antonio, L, Reyns, T, Fiers, T, Kema, I P, Kaufman, J M, Van De Merbel, N C & Lapauw, B 2025, 'SHBG Gene Polymorphisms and Their Influence on Serum SHBG, Total and Free Testosterone Concentrations in Men', Journal of Clinical Endocrinology and Metabolism, vol. 110, no. 3, pp. e641-e649. https://doi.org/10.1210/clinem/dgae280 – Name: DatePubCY Label: Publication Year Group: Date Data: 2025 – Name: Subset Label: Collection Group: HoldingsInfo Data: University of Groningen research database – Name: Subject Label: Subject Terms Group: Su Data: <searchLink fieldCode="DE" term="%22free+testosterone%22">free testosterone</searchLink><br /><searchLink fieldCode="DE" term="%22healthy+men%22">healthy men</searchLink><br /><searchLink fieldCode="DE" term="%22LC-MS%2FMS%22">LC-MS/MS</searchLink><br /><searchLink fieldCode="DE" term="%22polymorphisms%22">polymorphisms</searchLink><br /><searchLink fieldCode="DE" term="%22SHBG%22">SHBG</searchLink><br /><searchLink fieldCode="DE" term="%22testosterone%22">testosterone</searchLink> – Name: Abstract Label: Description Group: Ab Data: Context: Genetic variation in SHBG structure may affect estimates of sex steroid exposure by altering the affinity of the protein for its ligand. Consequently, free hormone calculations assuming constant binding affinity may, for certain genetic variations, lead to incorrect diagnoses if genetic variation is not taken into consideration. Objective: To investigate the effects of genetic variation in SHBG on calculated and measured serum free testosterone (T) in men. Design, setting and participants: Population-based sibling-pair study in 999 healthy men aged 25 to 45 (mean, 34.5) years. Main outcome measures: Genotyping using microarray (Illumina) for single-nucleotide polymorphism (SNPs) suggested to affect binding affinity and/or concentration of SHBG or T. SHBG concentrations were measured using immunoassay and in a subset (n = 32) by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Total T was measured using LC-MS/MS. Free T was calculated and in a subset (n = 314) measured directly using LC-MS/MS after equilibrium dialysis. Results: Allelic frequencies of analyzed SNPs ranged from 0.5% to 58.2%. Compared to wild-type, SHBG concentrations were lower in rs6258 heterozygotes (-24.7%; P <. 05) and higher in rs6259 heterozygotes, rs727428 homozygotes, and carriers of rs1799941 (+10.8 to 23.1%; all P <. 05). Total T was higher in rs727428 homozygotes and carriers of rs5934505, rs1799941and rs6259 (+3.9 to 21.4%; all P <. 05). No clear effects on measured free T were found, except for a trend toward higher values in rs6259 homozygotes, significant for calculated free T (+18.7%; P <. 05) in the larger global study population. Conclusion: In these men, analyzed SNPs were relatively prevalent and affected serum concentrations of total T and SHBG but not calculated or measured free T except for a higher trend in rs6259 homozygotes. – Name: TypeDocument Label: Document Type Group: TypDoc Data: article in journal/newspaper – Name: Format Label: File Description Group: SrcInfo Data: application/pdf – Name: Language Label: Language Group: Lang Data: English – Name: ISSN Label: ISSN Group: ISSN Data: 0021-972X<br />1945-7197 – Name: NoteTitleSource Label: Relation Group: SrcInfo Data: info:eu-repo/semantics/altIdentifier/pmid/38652149; info:eu-repo/semantics/altIdentifier/hdl/https://hdl.handle.net/11370/28d52265-4311-4754-8ff0-6a47ac595650; info:eu-repo/semantics/altIdentifier/pissn/0021-972X; info:eu-repo/semantics/altIdentifier/eissn/1945-7197 – Name: DOI Label: DOI Group: ID Data: 10.1210/clinem/dgae280 – Name: URL Label: Availability Group: URL Data: https://hdl.handle.net/11370/28d52265-4311-4754-8ff0-6a47ac595650<br />https://research.rug.nl/en/publications/28d52265-4311-4754-8ff0-6a47ac595650<br />https://doi.org/10.1210/clinem/dgae280<br />https://pure.rug.nl/ws/files/1316746356/dgae280.pdf<br />https://www.scopus.com/pages/publications/85218103018 – Name: Copyright Label: Rights Group: Cpyrght Data: info:eu-repo/semantics/openAccess ; https://www.rug.nl/library/open-access/article-25fa-pilot-end-user-agreement – Name: AN Label: Accession Number Group: ID Data: edsbas.7B4B8EE0 |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1210/clinem/dgae280 Languages: – Text: English Subjects: – SubjectFull: free testosterone Type: general – SubjectFull: healthy men Type: general – SubjectFull: LC-MS/MS Type: general – SubjectFull: polymorphisms Type: general – SubjectFull: SHBG Type: general – SubjectFull: testosterone Type: general Titles: – TitleFull: SHBG Gene Polymorphisms and Their Influence on Serum SHBG, Total and Free Testosterone Concentrations in Men Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Walravens, Joeri – PersonEntity: Name: NameFull: Sleumer, Bas – PersonEntity: Name: NameFull: Vos, Michel J. – PersonEntity: Name: NameFull: Snaterse, Gido – PersonEntity: Name: NameFull: Narinx, Nick – PersonEntity: Name: NameFull: Antonio, Leen – PersonEntity: Name: NameFull: Reyns, Tim – PersonEntity: Name: NameFull: Fiers, Tom – PersonEntity: Name: NameFull: Kema, Ido P. – PersonEntity: Name: NameFull: Kaufman, Jean Marc – PersonEntity: Name: NameFull: Van De Merbel, Nico C. – PersonEntity: Name: NameFull: Lapauw, Bruno IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 01 Type: published Y: 2025 Identifiers: – Type: issn-print Value: 0021972X – Type: issn-print Value: 19457197 – Type: issn-locals Value: edsbas – Type: issn-locals Value: edsbas.oa Titles: – TitleFull: Walravens, J, Sleumer, B, Vos, M J, Snaterse, G, Narinx, N, Antonio, L, Reyns, T, Fiers, T, Kema, I P, Kaufman, J M, Van De Merbel, N C & Lapauw, B 2025, 'SHBG Gene Polymorphisms and Their Influence on Serum SHBG, Total and Free Testosterone Concentrations in Men', Journal of Clinical Endocrinology and Metabolism, vol. 110, no. 3, pp. e641-e649. https://doi.org/10.1210/clinem/dgae280 Type: main |
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