Academic Journal
Branched actin polymerization drives invasive protrusion formation to promote myoblast fusion during mouse skeletal muscle regeneration
| Τίτλος: | Branched actin polymerization drives invasive protrusion formation to promote myoblast fusion during mouse skeletal muscle regeneration |
|---|---|
| Συγγραφείς: | Lu, Yue, Walji, Tezin, Pandey, Pratima, Zhou, Chuanli, Habela, Christa W, Snapper, Scott B, Li, Rong, Chen, Elizabeth H |
| Συνεισφορές: | American Heart Association, National Institute of General Medical Sciences |
| Πηγή: | eLife ; volume 14 ; ISSN 2050-084X |
| Στοιχεία εκδότη: | eLife Sciences Publications, Ltd |
| Έτος έκδοσης: | 2026 |
| Συλλογή: | eLife (E-Journal - via CrossRef) |
| Περιγραφή: | Skeletal muscle regeneration is a multistep process involving the activation, proliferation, differentiation, and fusion of muscle stem cells, known as satellite cells. Fusion of satellite cell-derived myoblasts (SCMs) is indispensable for generating the multinucleated, contractile myofibers during muscle repair. However, the molecular and cellular mechanisms underlying SCM fusion during muscle regeneration remain incompletely understood. Here, we reveal a critical role for branched actin polymerization in SCM fusion during mouse skeletal muscle regeneration. Using conditional knockouts of the Arp2/3 complex and its actin nucleation-promoting factors N-WASP and WAVE, we demonstrate that branched actin polymerization is specifically required for SCM fusion but dispensable for satellite cell proliferation, differentiation, and migration. We show that the N-WASP and WAVE complexes have partially redundant functions in regulating SCM fusion and that branched actin polymerization is essential for generating invasive protrusions at fusogenic synapses in SCMs. Together, our study identifies branched-actin regulators as key components of the myoblast fusion machinery and establishes invasive protrusion formation as a critical mechanism enabling myoblast fusion during skeletal muscle regeneration. |
| Τύπος εγγράφου: | article in journal/newspaper |
| Γλώσσα: | English |
| DOI: | 10.7554/elife.103550 |
| Διαθεσιμότητα: | https://doi.org/10.7554/elife.103550 https://cdn.elifesciences.org/articles/103550/elife-103550-v1.pdf https://cdn.elifesciences.org/articles/103550/elife-103550-v1.xml https://elifesciences.org/articles/103550 |
| Rights: | http://creativecommons.org/licenses/by/4.0/ ; http://creativecommons.org/licenses/by/4.0/ ; http://creativecommons.org/licenses/by/4.0/ |
| Αριθμός Καταχώρησης: | edsbas.6720B522 |
| Βάση Δεδομένων: | BASE |
| FullText | Text: Availability: 0 CustomLinks: – Url: https://doi.org/10.7554/elife.103550# Name: EDS - BASE (ns324271) Category: fullText Text: View record from BASE |
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| Items | – Name: Title Label: Title Group: Ti Data: Branched actin polymerization drives invasive protrusion formation to promote myoblast fusion during mouse skeletal muscle regeneration – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Lu%2C+Yue%22">Lu, Yue</searchLink><br /><searchLink fieldCode="AR" term="%22Walji%2C+Tezin%22">Walji, Tezin</searchLink><br /><searchLink fieldCode="AR" term="%22Pandey%2C+Pratima%22">Pandey, Pratima</searchLink><br /><searchLink fieldCode="AR" term="%22Zhou%2C+Chuanli%22">Zhou, Chuanli</searchLink><br /><searchLink fieldCode="AR" term="%22Habela%2C+Christa+W%22">Habela, Christa W</searchLink><br /><searchLink fieldCode="AR" term="%22Snapper%2C+Scott+B%22">Snapper, Scott B</searchLink><br /><searchLink fieldCode="AR" term="%22Li%2C+Rong%22">Li, Rong</searchLink><br /><searchLink fieldCode="AR" term="%22Chen%2C+Elizabeth+H%22">Chen, Elizabeth H</searchLink> – Name: Author Label: Contributors Group: Au Data: American Heart Association<br />National Institute of General Medical Sciences – Name: TitleSource Label: Source Group: Src Data: eLife ; volume 14 ; ISSN 2050-084X – Name: Publisher Label: Publisher Information Group: PubInfo Data: eLife Sciences Publications, Ltd – Name: DatePubCY Label: Publication Year Group: Date Data: 2026 – Name: Subset Label: Collection Group: HoldingsInfo Data: eLife (E-Journal - via CrossRef) – Name: Abstract Label: Description Group: Ab Data: Skeletal muscle regeneration is a multistep process involving the activation, proliferation, differentiation, and fusion of muscle stem cells, known as satellite cells. Fusion of satellite cell-derived myoblasts (SCMs) is indispensable for generating the multinucleated, contractile myofibers during muscle repair. However, the molecular and cellular mechanisms underlying SCM fusion during muscle regeneration remain incompletely understood. Here, we reveal a critical role for branched actin polymerization in SCM fusion during mouse skeletal muscle regeneration. Using conditional knockouts of the Arp2/3 complex and its actin nucleation-promoting factors N-WASP and WAVE, we demonstrate that branched actin polymerization is specifically required for SCM fusion but dispensable for satellite cell proliferation, differentiation, and migration. We show that the N-WASP and WAVE complexes have partially redundant functions in regulating SCM fusion and that branched actin polymerization is essential for generating invasive protrusions at fusogenic synapses in SCMs. Together, our study identifies branched-actin regulators as key components of the myoblast fusion machinery and establishes invasive protrusion formation as a critical mechanism enabling myoblast fusion during skeletal muscle regeneration. – Name: TypeDocument Label: Document Type Group: TypDoc Data: article in journal/newspaper – Name: Language Label: Language Group: Lang Data: English – Name: DOI Label: DOI Group: ID Data: 10.7554/elife.103550 – Name: URL Label: Availability Group: URL Data: https://doi.org/10.7554/elife.103550<br />https://cdn.elifesciences.org/articles/103550/elife-103550-v1.pdf<br />https://cdn.elifesciences.org/articles/103550/elife-103550-v1.xml<br />https://elifesciences.org/articles/103550 – Name: Copyright Label: Rights Group: Cpyrght Data: http://creativecommons.org/licenses/by/4.0/ ; http://creativecommons.org/licenses/by/4.0/ ; http://creativecommons.org/licenses/by/4.0/ – Name: AN Label: Accession Number Group: ID Data: edsbas.6720B522 |
| PLink | https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edsbas&AN=edsbas.6720B522 |
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