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Development and characterization of reverse genetics systems of feline infectious peritonitis virus for antiviral research

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Τίτλος: Development and characterization of reverse genetics systems of feline infectious peritonitis virus for antiviral research
Συγγραφείς: Gu, Guoqian, Fung, To Sing, Hung, Wong Tsz, Osterrieder, Nikolaus, Go, Yun Young
Πηγή: http://lobid.org/resources/99370671682706441#!, 55(1):124.
Έτος έκδοσης: 2024
Συλλογή: Publisso (ZB MED-Publikationsportal Lebenswissenschaften)
Θεματικοί όροι: reverse genetics, antiviral compounds, Coronavirus, Feline/genetics [MeSH], Cats [MeSH], Cell Line [MeSH], Feline/physiology [MeSH], Animals [MeSH], Feline infectious peritonitis virus, Virus Replication [MeSH], replicon, Antiviral Agents/pharmacology [MeSH], high-content screening, recombinant viruses, DNA, Complementary/genetics [MeSH], Feline Infectious Peritonitis/virology [MeSH], Reverse Genetics/methods [MeSH], Research Article
Περιγραφή: Abstract Feline infectious peritonitis (FIP) is a lethal, immune-mediated disease in cats caused by feline infectious peritonitis virus (FIPV), a biotype of feline coronavirus (FCoV). In contrast to feline enteric coronavirus (FECV), which exclusively infects enterocytes and causes diarrhea, FIPV specifically targets macrophages, resulting in the development of FIP. The transmission and infection mechanisms of this complex, invariably fatal disease remain unclear, with no effective vaccines or approved drugs for its prevention or control. In this study, a full-length infectious cDNA clone of the wild-type FIPV WSU79-1146 strain was constructed to generate recombinant FIPV (rFIPV-WT), which exhibited similar growth kinetics and produced infectious virus titres comparable to those of the parental wild-type virus. In addition, the superfold green fluorescent protein (msfGFP) and Renilla luciferase (Rluc) reporter genes were incorporated into the rFIPV-WT cDNA construct to generate reporter rFIPV-msfGFP and rFIPV-Rluc viruses. While the growth characteristics of the rFIPV-msfGFP virus were similar to those of its parental rFIPV-WT, the rFIPV-Rluc virus replicated more slowly, resulting in the formation of smaller plaques than did the rFIPV-WT and rFIPV-msfGFP viruses. In addition, by replacing the S, E, M, and ORF3abc genes with msfGFP and Rluc genes, the replicon systems repFIPV-msfGFP and repFIPV-Rluc were generated on the basis of the cDNA construct of rFIPV-WT. Last, the use of reporter recombinant viruses and replicons in antiviral screening assays demonstrated their high sensitivity for quantifying the antiviral effectiveness of the tested compounds. This integrated system promises to significantly streamline the investigation of virus replication within host cells, ...
Τύπος εγγράφου: article in journal/newspaper
Γλώσσα: English
Relation: https://repository.publisso.de/resource/frl:6505010; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11934533/
DOI: 10.1186/s13567-024-01373-z
Διαθεσιμότητα: https://repository.publisso.de/resource/frl:6505010
https://doi.org/10.1186/s13567-024-01373-z
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11934533/
Rights: https://creativecommons.org/licenses/by/4.0/
Αριθμός Καταχώρησης: edsbas.5E58F737
Βάση Δεδομένων: BASE
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  Data: Development and characterization of reverse genetics systems of feline infectious peritonitis virus for antiviral research
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  Data: <searchLink fieldCode="AR" term="%22Gu%2C+Guoqian%22">Gu, Guoqian</searchLink><br /><searchLink fieldCode="AR" term="%22Fung%2C+To+Sing%22">Fung, To Sing</searchLink><br /><searchLink fieldCode="AR" term="%22Hung%2C+Wong+Tsz%22">Hung, Wong Tsz</searchLink><br /><searchLink fieldCode="AR" term="%22Osterrieder%2C+Nikolaus%22">Osterrieder, Nikolaus</searchLink><br /><searchLink fieldCode="AR" term="%22Go%2C+Yun+Young%22">Go, Yun Young</searchLink>
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  Data: <i>http://lobid.org/resources/99370671682706441#!, 55(1):124</i>.
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  Data: 2024
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  Data: Publisso (ZB MED-Publikationsportal Lebenswissenschaften)
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  Data: <searchLink fieldCode="DE" term="%22reverse+genetics%22">reverse genetics</searchLink><br /><searchLink fieldCode="DE" term="%22antiviral+compounds%22">antiviral compounds</searchLink><br /><searchLink fieldCode="DE" term="%22Coronavirus%22">Coronavirus</searchLink><br /><searchLink fieldCode="DE" term="%22Feline%2Fgenetics+[MeSH]%22">Feline/genetics [MeSH]</searchLink><br /><searchLink fieldCode="DE" term="%22Cats+[MeSH]%22">Cats [MeSH]</searchLink><br /><searchLink fieldCode="DE" term="%22Cell+Line+[MeSH]%22">Cell Line [MeSH]</searchLink><br /><searchLink fieldCode="DE" term="%22Feline%2Fphysiology+[MeSH]%22">Feline/physiology [MeSH]</searchLink><br /><searchLink fieldCode="DE" term="%22Animals+[MeSH]%22">Animals [MeSH]</searchLink><br /><searchLink fieldCode="DE" term="%22Feline+infectious+peritonitis+virus%22">Feline infectious peritonitis virus</searchLink><br /><searchLink fieldCode="DE" term="%22Virus+Replication+[MeSH]%22">Virus Replication [MeSH]</searchLink><br /><searchLink fieldCode="DE" term="%22replicon%22">replicon</searchLink><br /><searchLink fieldCode="DE" term="%22Antiviral+Agents%2Fpharmacology+[MeSH]%22">Antiviral Agents/pharmacology [MeSH]</searchLink><br /><searchLink fieldCode="DE" term="%22high-content+screening%22">high-content screening</searchLink><br /><searchLink fieldCode="DE" term="%22recombinant+viruses%22">recombinant viruses</searchLink><br /><searchLink fieldCode="DE" term="%22DNA%22">DNA</searchLink><br /><searchLink fieldCode="DE" term="%22Complementary%2Fgenetics+[MeSH]%22">Complementary/genetics [MeSH]</searchLink><br /><searchLink fieldCode="DE" term="%22Feline+Infectious+Peritonitis%2Fvirology+[MeSH]%22">Feline Infectious Peritonitis/virology [MeSH]</searchLink><br /><searchLink fieldCode="DE" term="%22Reverse+Genetics%2Fmethods+[MeSH]%22">Reverse Genetics/methods [MeSH]</searchLink><br /><searchLink fieldCode="DE" term="%22Research+Article%22">Research Article</searchLink>
– Name: Abstract
  Label: Description
  Group: Ab
  Data: <jats:title>Abstract</jats:title> <jats:p>Feline infectious peritonitis (FIP) is a lethal, immune-mediated disease in cats caused by feline infectious peritonitis virus (FIPV), a biotype of feline coronavirus (FCoV). In contrast to feline enteric coronavirus (FECV), which exclusively infects enterocytes and causes diarrhea, FIPV specifically targets macrophages, resulting in the development of FIP. The transmission and infection mechanisms of this complex, invariably fatal disease remain unclear, with no effective vaccines or approved drugs for its prevention or control. In this study, a full-length infectious cDNA clone of the wild-type FIPV WSU79-1146 strain was constructed to generate recombinant FIPV (rFIPV-WT), which exhibited similar growth kinetics and produced infectious virus titres comparable to those of the parental wild-type virus. In addition, the superfold green fluorescent protein (<jats:italic>msfGFP</jats:italic>) and <jats:italic>Renilla</jats:italic> luciferase (<jats:italic>Rluc</jats:italic>) reporter genes were incorporated into the rFIPV-WT cDNA construct to generate reporter rFIPV-msfGFP and rFIPV-Rluc viruses. While the growth characteristics of the rFIPV-msfGFP virus were similar to those of its parental rFIPV-WT, the rFIPV-Rluc virus replicated more slowly, resulting in the formation of smaller plaques than did the rFIPV-WT and rFIPV-msfGFP viruses. In addition, by replacing the S, E, M, and ORF3abc genes with <jats:italic>msfGFP</jats:italic> and <jats:italic>Rluc</jats:italic> genes, the replicon systems repFIPV-msfGFP and repFIPV-Rluc were generated on the basis of the cDNA construct of rFIPV-WT. Last, the use of reporter recombinant viruses and replicons in antiviral screening assays demonstrated their high sensitivity for quantifying the antiviral effectiveness of the tested compounds. This integrated system promises to significantly streamline the investigation of virus replication within host cells, ...
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        Value: 10.1186/s13567-024-01373-z
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      – Text: English
    Subjects:
      – SubjectFull: reverse genetics
        Type: general
      – SubjectFull: antiviral compounds
        Type: general
      – SubjectFull: Coronavirus
        Type: general
      – SubjectFull: Feline/genetics [MeSH]
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