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Assessment of disease activity in patients with rheumatoid arthritis using plasma tumour M2-pyruvate kinase test

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Title: Assessment of disease activity in patients with rheumatoid arthritis using plasma tumour M2-pyruvate kinase test
Contributors: Sung Soo Ahn, Hye Min Kim, Younhee Park, Kim, Hye Min
Publisher Information: Frontiers Research Foundation
Publication Year: 2022
Subject Terms: Arthritis, Rheumatoid* / diagnosis, Rheumatoid* / pathology, Blood Sedimentation, C-Reactive Protein / metabolism, Humans, Neoplasms / diagnosis, Neoplasms / pathology, Osteoarthritis* / diagnosis, Osteoarthritis* / metabolism, Osteoarthritis* / pathology, Pyruvate Kinase, Synovial Membrane / pathology, biomarker, disease activity, predictor, rheumatoid arthritis, tumour M2-pyruvate kinase
Description: Background: Pyruvate kinase M2 (PKM2) is an enzyme that regulates the final process of glycolysis and exists in tetrameric and dimeric forms. The dimeric form of PKM2, also known as tumour M2-PK, increases when aerobic glycolysis is augmented, a feature observed in rheumatoid arthritis (RA). We investigated whether plasma tumour M2-PK is elevated in patients with RA and whether its levels correlate with disease activity. Methods: Plasma levels of tumour M2-PK were measured for patients with RA (n=151), those with osteoarthritis (OA) (n=37), and controls (n=37). We evaluated the association between plasma tumour M2-PK and continuous variables using Pearson's correlation analysis, and multivariate logistic regression analysis to determine the association between plasma tumour M2-PK and disease activity status. Knee synovial tissue blocks from patients with RA and OA were subjected to real-time quantitative PCR (qPCR) using two different primers for PKM2 and tumour M2-PK immunohistochemical (IHC) staining. Results: The tumour M2-PK level significantly correlated with the disease activity score in 28 joints (DAS28)-erythrocyte sedimentation rate (ESR) (r=0.546, p<0.001) and DAS28-C-reactive protein (CRP) (r=0.589, p<0.001). Moreover, repeat testing of tumour M2-PK levels in 20 patients revealed a significant decline in tumour M2-PK levels after reduction in inflammation (p<0.001). Area under the receiver operating characteristic curve (AUROC) analysis demonstrated that upon incorporation of tumour M2-PK, ESR, and CRP, the area under the curve was 0.962 for distinguishing moderate/high from remission/low disease activity. Adjusted logistic regression also revealed that a tumour M2-PK >43.9 U/mL (OR 3.672, p=0.042) independently predicted moderate/high disease activity status. Furthermore, tumour M2-PK levels in patients with RA were significantly higher than in those with OA and controls (all p<0.001). However, no differences were found in PKM2 expression in RA and OA synovial tissues as assessed by ...
Document Type: article in journal/newspaper
Language: English
Relation: FRONTIERS IN IMMUNOLOGY; J03075; https://ir.ymlib.yonsei.ac.kr/handle/22282913/191814; T202203321
DOI: 10.3389/fimmu.2022.901555
Availability: https://ir.ymlib.yonsei.ac.kr/handle/22282913/191814
https://doi.org/10.3389/fimmu.2022.901555
Rights: CC BY-NC-ND 2.0 KR
Accession Number: edsbas.5D5C8CDC
Database: BASE
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  Label: Title
  Group: Ti
  Data: Assessment of disease activity in patients with rheumatoid arthritis using plasma tumour M2-pyruvate kinase test
– Name: Author
  Label: Contributors
  Group: Au
  Data: Sung Soo Ahn&lt;br /&gt;Hye Min Kim&lt;br /&gt;Younhee Park&lt;br /&gt;Kim, Hye Min
– Name: Publisher
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  Data: Frontiers Research Foundation
– Name: DatePubCY
  Label: Publication Year
  Group: Date
  Data: 2022
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  Data: &lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Arthritis%22&quot;&gt;Arthritis&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Rheumatoid*+%2F+diagnosis%22&quot;&gt;Rheumatoid* / diagnosis&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Rheumatoid*+%2F+pathology%22&quot;&gt;Rheumatoid* / pathology&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Blood+Sedimentation%22&quot;&gt;Blood Sedimentation&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22C-Reactive+Protein+%2F+metabolism%22&quot;&gt;C-Reactive Protein / metabolism&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Humans%22&quot;&gt;Humans&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Neoplasms+%2F+diagnosis%22&quot;&gt;Neoplasms / diagnosis&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Neoplasms+%2F+pathology%22&quot;&gt;Neoplasms / pathology&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Osteoarthritis*+%2F+diagnosis%22&quot;&gt;Osteoarthritis* / diagnosis&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Osteoarthritis*+%2F+metabolism%22&quot;&gt;Osteoarthritis* / metabolism&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Osteoarthritis*+%2F+pathology%22&quot;&gt;Osteoarthritis* / pathology&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Pyruvate+Kinase%22&quot;&gt;Pyruvate Kinase&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Synovial+Membrane+%2F+pathology%22&quot;&gt;Synovial Membrane / pathology&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22biomarker%22&quot;&gt;biomarker&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22disease+activity%22&quot;&gt;disease activity&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22predictor%22&quot;&gt;predictor&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22rheumatoid+arthritis%22&quot;&gt;rheumatoid arthritis&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22tumour+M2-pyruvate+kinase%22&quot;&gt;tumour M2-pyruvate kinase&lt;/searchLink&gt;
– Name: Abstract
  Label: Description
  Group: Ab
  Data: Background: Pyruvate kinase M2 (PKM2) is an enzyme that regulates the final process of glycolysis and exists in tetrameric and dimeric forms. The dimeric form of PKM2, also known as tumour M2-PK, increases when aerobic glycolysis is augmented, a feature observed in rheumatoid arthritis (RA). We investigated whether plasma tumour M2-PK is elevated in patients with RA and whether its levels correlate with disease activity. Methods: Plasma levels of tumour M2-PK were measured for patients with RA (n=151), those with osteoarthritis (OA) (n=37), and controls (n=37). We evaluated the association between plasma tumour M2-PK and continuous variables using Pearson&#39;s correlation analysis, and multivariate logistic regression analysis to determine the association between plasma tumour M2-PK and disease activity status. Knee synovial tissue blocks from patients with RA and OA were subjected to real-time quantitative PCR (qPCR) using two different primers for PKM2 and tumour M2-PK immunohistochemical (IHC) staining. Results: The tumour M2-PK level significantly correlated with the disease activity score in 28 joints (DAS28)-erythrocyte sedimentation rate (ESR) (r=0.546, p&lt;0.001) and DAS28-C-reactive protein (CRP) (r=0.589, p&lt;0.001). Moreover, repeat testing of tumour M2-PK levels in 20 patients revealed a significant decline in tumour M2-PK levels after reduction in inflammation (p&lt;0.001). Area under the receiver operating characteristic curve (AUROC) analysis demonstrated that upon incorporation of tumour M2-PK, ESR, and CRP, the area under the curve was 0.962 for distinguishing moderate/high from remission/low disease activity. Adjusted logistic regression also revealed that a tumour M2-PK &gt;43.9 U/mL (OR 3.672, p=0.042) independently predicted moderate/high disease activity status. Furthermore, tumour M2-PK levels in patients with RA were significantly higher than in those with OA and controls (all p&lt;0.001). However, no differences were found in PKM2 expression in RA and OA synovial tissues as assessed by ...
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  Data: FRONTIERS IN IMMUNOLOGY; J03075; https://ir.ymlib.yonsei.ac.kr/handle/22282913/191814; T202203321
– Name: DOI
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  Data: 10.3389/fimmu.2022.901555
– Name: URL
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  Data: https://ir.ymlib.yonsei.ac.kr/handle/22282913/191814&lt;br /&gt;https://doi.org/10.3389/fimmu.2022.901555
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RecordInfo BibRecord:
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      – Type: doi
        Value: 10.3389/fimmu.2022.901555
    Languages:
      – Text: English
    Subjects:
      – SubjectFull: Arthritis
        Type: general
      – SubjectFull: Rheumatoid* / diagnosis
        Type: general
      – SubjectFull: Rheumatoid* / pathology
        Type: general
      – SubjectFull: Blood Sedimentation
        Type: general
      – SubjectFull: C-Reactive Protein / metabolism
        Type: general
      – SubjectFull: Humans
        Type: general
      – SubjectFull: Neoplasms / diagnosis
        Type: general
      – SubjectFull: Neoplasms / pathology
        Type: general
      – SubjectFull: Osteoarthritis* / diagnosis
        Type: general
      – SubjectFull: Osteoarthritis* / metabolism
        Type: general
      – SubjectFull: Osteoarthritis* / pathology
        Type: general
      – SubjectFull: Pyruvate Kinase
        Type: general
      – SubjectFull: Synovial Membrane / pathology
        Type: general
      – SubjectFull: biomarker
        Type: general
      – SubjectFull: disease activity
        Type: general
      – SubjectFull: predictor
        Type: general
      – SubjectFull: rheumatoid arthritis
        Type: general
      – SubjectFull: tumour M2-pyruvate kinase
        Type: general
    Titles:
      – TitleFull: Assessment of disease activity in patients with rheumatoid arthritis using plasma tumour M2-pyruvate kinase test
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            NameFull: Sung Soo Ahn
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            NameFull: Hye Min Kim
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            NameFull: Younhee Park
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            NameFull: Kim, Hye Min
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              Type: published
              Y: 2022
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