Academic Journal
Non-invasive hydrodynamic imaging in plant roots at cellular resolution
| Title: | Non-invasive hydrodynamic imaging in plant roots at cellular resolution |
|---|---|
| Authors: | Pascut, Flavius C, Couvreur, Valentin, Dietrich, Daniela, Leftley, Nicky, Reyt, Guilhem, Boursiac, Yann, Calvo-Polanco, Monica, Casimiro, Ilda, Maurel, Christophe, Salt, David E, Draye, Xavier, Wells, Darren M, Bennett, Malcolm J, Webb, Kevin F |
| Source: | Pascut, F C, Couvreur, V, Dietrich, D, Leftley, N, Reyt, G, Boursiac, Y, Calvo-Polanco, M, Casimiro, I, Maurel, C, Salt, D E, Draye, X, Wells, D M, Bennett, M J & Webb, K F 2021, 'Non-invasive hydrodynamic imaging in plant roots at cellular resolution', Nature Communications, vol. 12, no. 1, 4682, pp. 4682. https://doi.org/10.1038/s41467-021-24913-z |
| Publication Year: | 2021 |
| Collection: | University of Bristol: Bristol Reserach |
| Subject Terms: | Arabidopsis/anatomy & histology, Biological Transport, Hydrodynamics, Models, Biological, Mutation, Plant Roots/anatomy & histology, Plant Shoots/metabolism, Plant Stomata/metabolism, Spectrum Analysis, Raman, Water/metabolism, Xylem/metabolism |
| Description: | A key impediment to studying water-related mechanisms in plants is the inability to non-invasively image water fluxes in cells at high temporal and spatial resolution. Here, we report that Raman microspectroscopy, complemented by hydrodynamic modelling, can achieve this goal - monitoring hydrodynamics within living root tissues at cell- and sub-second-scale resolutions. Raman imaging of water-transporting xylem vessels in Arabidopsis thaliana mutant roots reveals faster xylem water transport in endodermal diffusion barrier mutants. Furthermore, transverse line scans across the root suggest water transported via the root xylem does not re-enter outer root tissues nor the surrounding soil when en-route to shoot tissues if endodermal diffusion barriers are intact, thereby separating 'two water worlds'. |
| Document Type: | article in journal/newspaper |
| File Description: | application/pdf |
| Language: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/34344886; info:eu-repo/semantics/altIdentifier/hdl/https://hdl.handle.net/1983/c8e07772-0add-426c-9cb9-3d1812e8ccd7 |
| DOI: | 10.1038/s41467-021-24913-z |
| Availability: | https://hdl.handle.net/1983/c8e07772-0add-426c-9cb9-3d1812e8ccd7 https://research-information.bris.ac.uk/en/publications/c8e07772-0add-426c-9cb9-3d1812e8ccd7 https://doi.org/10.1038/s41467-021-24913-z https://research-information.bris.ac.uk/ws/files/288634693/Full_text_PDF_final_published_version_.pdf |
| Rights: | info:eu-repo/semantics/openAccess ; http://creativecommons.org/licenses/by/4.0/ |
| Accession Number: | edsbas.38B38B27 |
| Database: | BASE |
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