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A conserved choreography of mRNAs at centrosomes reveals a localization mechanism involving active polysome transport

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Τίτλος: A conserved choreography of mRNAs at centrosomes reveals a localization mechanism involving active polysome transport
Συγγραφείς: Safieddine, Adham, Coleno, Emeline, Traboulsi, Abdel-Meneem, Kwon, Oh Sung, Lionneton, Frederic, Georget, Virginie, Robert, Marie-Cécile, Gostan, Thierry, Lecellier, Charles, Salloum, Soha, Chouaib, Racha, Pichon, Xavier, Le Hir, Hervé, Zibara, Kazem, Peter, Marion, Bertrand, Edouard
Συνεισφορές: Institut de Génétique Moléculaire de Montpellier (IGMM), Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM), Institut de biologie de l'ENS Paris (UMR 8197/1024) (IBENS), Département de Biologie - ENS Paris, École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), BioCampus Montpellier (BCM), Université Montpellier 1 (UM1)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Lebanese University Beirut (LU)
Πηγή: https://hal.archives-ouvertes.fr/hal-03014936 ; 2020.
Στοιχεία εκδότη: HAL CCSD
Έτος έκδοσης: 2020
Συλλογή: Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe)
Θεματικοί όροι: high-throughput screening, ASPM, smFISH, cotranslational targeting, conserved polysome choreography at centrosomes mRNA localization, local translation, centrosomes, polysome imaging, NUMA1, [SDV]Life Sciences [q-bio]
Περιγραφή: Local translation allows for a spatial control of gene expression. Here, we used high-throughput smFISH to screen centrosomal protein-coding genes, and we describe 8 human mRNAs accumulating at centrosomes. These mRNAs localize at different stages during cell cycle with a remarkable choreography, indicating a finely regulated translational program at centrosomes. Interestingly, drug treatments and reporter analyses revealed a common translation-dependent localization mechanism requiring the nascent protein. Using ASPM and NUMA1 as models, single mRNA and polysome imaging revealed active movements of endogenous polysomes towards the centrosome at the onset of mitosis, when these mRNAs start localizing. ASPM polysomes associate with microtubules and localize by either motor-driven transport or microtubule pulling. Remarkably, the Drosophila orthologs of the human centrosomal mRNAs also localize to centrosomes and also require translation. These data identify a conserved family of centrosomal mRNAs that localize by active polysomes transport mediated by nascent proteins. ; Local translation allows for a spatial control of gene expression. Here, we used high-throughput smFISH to screen centrosomal protein-coding genes, and we describe 8 human mRNAs accumulating at centrosomes. These mRNAs localize at different stages during cell cycle with a remarkable choreography, indicating a finely regulated translational program at centrosomes. Interestingly, drug treatments and reporter analyses revealed a common translation-dependent localization mechanism requiring the nascent protein. Using ASPM and NUMA1 as models, single mRNA and polysome imaging revealed active movements of endogenous polysomes towards the centrosome at the onset of mitosis, when these mRNAs start localizing. ASPM polysomes associate with microtubules and localize by either motor-driven transport or microtubule pulling. Remarkably, the Drosophila orthologs of the human centrosomal mRNAs also localize to centrosomes and also require translation. These ...
Τύπος εγγράφου: report
Γλώσσα: English
Relation: hal-03014936; https://hal.archives-ouvertes.fr/hal-03014936; https://hal.archives-ouvertes.fr/hal-03014936/document; https://hal.archives-ouvertes.fr/hal-03014936/file/2020.09.04.282038v1.full.pdf; BIORXIV: 2020.09.04.282038
DOI: 10.1101/2020.09.04.282038
Διαθεσιμότητα: https://hal.archives-ouvertes.fr/hal-03014936
https://hal.archives-ouvertes.fr/hal-03014936/document
https://hal.archives-ouvertes.fr/hal-03014936/file/2020.09.04.282038v1.full.pdf
https://doi.org/10.1101/2020.09.04.282038
Rights: info:eu-repo/semantics/OpenAccess
Αριθμός Καταχώρησης: edsbas.326FC79B
Βάση Δεδομένων: BASE
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  – Url: https://hal.archives-ouvertes.fr/hal-03014936#
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  Data: A conserved choreography of mRNAs at centrosomes reveals a localization mechanism involving active polysome transport
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  Data: <searchLink fieldCode="AR" term="%22Safieddine%2C+Adham%22">Safieddine, Adham</searchLink><br /><searchLink fieldCode="AR" term="%22Coleno%2C+Emeline%22">Coleno, Emeline</searchLink><br /><searchLink fieldCode="AR" term="%22Traboulsi%2C+Abdel-Meneem%22">Traboulsi, Abdel-Meneem</searchLink><br /><searchLink fieldCode="AR" term="%22Kwon%2C+Oh+Sung%22">Kwon, Oh Sung</searchLink><br /><searchLink fieldCode="AR" term="%22Lionneton%2C+Frederic%22">Lionneton, Frederic</searchLink><br /><searchLink fieldCode="AR" term="%22Georget%2C+Virginie%22">Georget, Virginie</searchLink><br /><searchLink fieldCode="AR" term="%22Robert%2C+Marie-Cécile%22">Robert, Marie-Cécile</searchLink><br /><searchLink fieldCode="AR" term="%22Gostan%2C+Thierry%22">Gostan, Thierry</searchLink><br /><searchLink fieldCode="AR" term="%22Lecellier%2C+Charles%22">Lecellier, Charles</searchLink><br /><searchLink fieldCode="AR" term="%22Salloum%2C+Soha%22">Salloum, Soha</searchLink><br /><searchLink fieldCode="AR" term="%22Chouaib%2C+Racha%22">Chouaib, Racha</searchLink><br /><searchLink fieldCode="AR" term="%22Pichon%2C+Xavier%22">Pichon, Xavier</searchLink><br /><searchLink fieldCode="AR" term="%22Le+Hir%2C+Hervé%22">Le Hir, Hervé</searchLink><br /><searchLink fieldCode="AR" term="%22Zibara%2C+Kazem%22">Zibara, Kazem</searchLink><br /><searchLink fieldCode="AR" term="%22Peter%2C+Marion%22">Peter, Marion</searchLink><br /><searchLink fieldCode="AR" term="%22Bertrand%2C+Edouard%22">Bertrand, Edouard</searchLink>
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  Data: Institut de Génétique Moléculaire de Montpellier (IGMM)<br />Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)<br />Institut de biologie de l'ENS Paris (UMR 8197/1024) (IBENS)<br />Département de Biologie - ENS Paris<br />École normale supérieure - Paris (ENS Paris)<br />Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-École normale supérieure - Paris (ENS Paris)<br />Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)<br />BioCampus Montpellier (BCM)<br />Université Montpellier 1 (UM1)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)<br />Lebanese University Beirut (LU)
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  Data: <i>https://hal.archives-ouvertes.fr/hal-03014936 ; 2020</i>.
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  Data: Local translation allows for a spatial control of gene expression. Here, we used high-throughput smFISH to screen centrosomal protein-coding genes, and we describe 8 human mRNAs accumulating at centrosomes. These mRNAs localize at different stages during cell cycle with a remarkable choreography, indicating a finely regulated translational program at centrosomes. Interestingly, drug treatments and reporter analyses revealed a common translation-dependent localization mechanism requiring the nascent protein. Using ASPM and NUMA1 as models, single mRNA and polysome imaging revealed active movements of endogenous polysomes towards the centrosome at the onset of mitosis, when these mRNAs start localizing. ASPM polysomes associate with microtubules and localize by either motor-driven transport or microtubule pulling. Remarkably, the Drosophila orthologs of the human centrosomal mRNAs also localize to centrosomes and also require translation. These data identify a conserved family of centrosomal mRNAs that localize by active polysomes transport mediated by nascent proteins. ; Local translation allows for a spatial control of gene expression. Here, we used high-throughput smFISH to screen centrosomal protein-coding genes, and we describe 8 human mRNAs accumulating at centrosomes. These mRNAs localize at different stages during cell cycle with a remarkable choreography, indicating a finely regulated translational program at centrosomes. Interestingly, drug treatments and reporter analyses revealed a common translation-dependent localization mechanism requiring the nascent protein. Using ASPM and NUMA1 as models, single mRNA and polysome imaging revealed active movements of endogenous polysomes towards the centrosome at the onset of mitosis, when these mRNAs start localizing. ASPM polysomes associate with microtubules and localize by either motor-driven transport or microtubule pulling. Remarkably, the Drosophila orthologs of the human centrosomal mRNAs also localize to centrosomes and also require translation. These ...
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  Data: hal-03014936; https://hal.archives-ouvertes.fr/hal-03014936; https://hal.archives-ouvertes.fr/hal-03014936/document; https://hal.archives-ouvertes.fr/hal-03014936/file/2020.09.04.282038v1.full.pdf; BIORXIV: 2020.09.04.282038
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