Academic Journal
SYNCAS-mediated CRISPR-Cas9 genome editing in the Jewel wasp, Nasonia vitripennis
| Τίτλος: | SYNCAS-mediated CRISPR-Cas9 genome editing in the Jewel wasp, Nasonia vitripennis |
|---|---|
| Συγγραφείς: | Guerra, Filippo, De Rouck, Sander, Verhulst, Eveline C. |
| Πηγή: | Insect Molecular Biology 35 (2026) 1 ; ISSN: 0962-1075 |
| Έτος έκδοσης: | 2026 |
| Συλλογή: | Wageningen UR (University & Research Centre): Digital Library |
| Θεματικοί όροι: | BAPC, Nasonia vitripennis, SYNCAS, gene editing, saponin |
| Περιγραφή: | Genetic engineering is a formidable approach to studying biology. The development of CRISPR-Cas9 has allowed the genetic engineering of insect species from several orders, and in some species, this tool is used routinely for genetic research. However, insect gene editing often relies on the delivery of CRISPR-Cas9 components via embryo injection. This technique has a limitation: some species lay their eggs inside hard substrates or living hosts, making embryo collection impossible or labour intensive. Recently, a variety of techniques that exploit maternal injection of nucleases have been developed to circumvent embryo injection. Yet, despite this variety of maternal delivery techniques, some insects remain refractory to gene editing. One of these is the parasitoid wasp, Nasonia vitripennis, an important hymenopteran model species. In this study, a recently developed method termed SYNCAS was used to perform knock-out (KO) of the cinnabar gene in this wasp, obtaining KO efficiencies up to 10 times higher than reported for other maternal injection approaches. We found up to 2.73% of all offspring to display a KO phenotype, and we obtained up to 68 KO offspring per 100 injected mothers. The optimal timing of injection and provision of hosts for egg laying was determined. With this protocol, routine applications of CRISPR-Cas9 become feasible in this species, allowing reverse genetics studies of genes with unknown associated phenotypes and paving the way for more advanced editing techniques. |
| Τύπος εγγράφου: | article in journal/newspaper |
| Περιγραφή αρχείου: | application/pdf |
| Γλώσσα: | English |
| Relation: | https://edepot.wur.nl/698620 |
| DOI: | 10.1111/imb.70002 |
| Διαθεσιμότητα: | https://research.wur.nl/en/publications/syncas-mediated-crispr-cas9-genome-editing-in-the-jewel-wasp-naso https://doi.org/10.1111/imb.70002 https://edepot.wur.nl/698620 |
| Rights: | https://creativecommons.org/licenses/by/4.0/ ; Wageningen University & Research |
| Αριθμός Καταχώρησης: | edsbas.280B6914 |
| Βάση Δεδομένων: | BASE |
| FullText | Text: Availability: 0 CustomLinks: – Url: https://research.wur.nl/en/publications/syncas-mediated-crispr-cas9-genome-editing-in-the-jewel-wasp-naso# Name: EDS - BASE (ns324271) Category: fullText Text: View record from BASE |
|---|---|
| Header | DbId: edsbas DbLabel: BASE An: edsbas.280B6914 RelevancyScore: 1011 AccessLevel: 3 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 1010.63568115234 |
| IllustrationInfo | |
| Items | – Name: Title Label: Title Group: Ti Data: SYNCAS-mediated CRISPR-Cas9 genome editing in the Jewel wasp, Nasonia vitripennis – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Guerra%2C+Filippo%22">Guerra, Filippo</searchLink><br /><searchLink fieldCode="AR" term="%22De+Rouck%2C+Sander%22">De Rouck, Sander</searchLink><br /><searchLink fieldCode="AR" term="%22Verhulst%2C+Eveline+C%2E%22">Verhulst, Eveline C.</searchLink> – Name: TitleSource Label: Source Group: Src Data: Insect Molecular Biology 35 (2026) 1 ; ISSN: 0962-1075 – Name: DatePubCY Label: Publication Year Group: Date Data: 2026 – Name: Subset Label: Collection Group: HoldingsInfo Data: Wageningen UR (University & Research Centre): Digital Library – Name: Subject Label: Subject Terms Group: Su Data: <searchLink fieldCode="DE" term="%22BAPC%22">BAPC</searchLink><br /><searchLink fieldCode="DE" term="%22Nasonia+vitripennis%22">Nasonia vitripennis</searchLink><br /><searchLink fieldCode="DE" term="%22SYNCAS%22">SYNCAS</searchLink><br /><searchLink fieldCode="DE" term="%22gene+editing%22">gene editing</searchLink><br /><searchLink fieldCode="DE" term="%22saponin%22">saponin</searchLink> – Name: Abstract Label: Description Group: Ab Data: Genetic engineering is a formidable approach to studying biology. The development of CRISPR-Cas9 has allowed the genetic engineering of insect species from several orders, and in some species, this tool is used routinely for genetic research. However, insect gene editing often relies on the delivery of CRISPR-Cas9 components via embryo injection. This technique has a limitation: some species lay their eggs inside hard substrates or living hosts, making embryo collection impossible or labour intensive. Recently, a variety of techniques that exploit maternal injection of nucleases have been developed to circumvent embryo injection. Yet, despite this variety of maternal delivery techniques, some insects remain refractory to gene editing. One of these is the parasitoid wasp, Nasonia vitripennis, an important hymenopteran model species. In this study, a recently developed method termed SYNCAS was used to perform knock-out (KO) of the cinnabar gene in this wasp, obtaining KO efficiencies up to 10 times higher than reported for other maternal injection approaches. We found up to 2.73% of all offspring to display a KO phenotype, and we obtained up to 68 KO offspring per 100 injected mothers. The optimal timing of injection and provision of hosts for egg laying was determined. With this protocol, routine applications of CRISPR-Cas9 become feasible in this species, allowing reverse genetics studies of genes with unknown associated phenotypes and paving the way for more advanced editing techniques. – Name: TypeDocument Label: Document Type Group: TypDoc Data: article in journal/newspaper – Name: Format Label: File Description Group: SrcInfo Data: application/pdf – Name: Language Label: Language Group: Lang Data: English – Name: NoteTitleSource Label: Relation Group: SrcInfo Data: https://edepot.wur.nl/698620 – Name: DOI Label: DOI Group: ID Data: 10.1111/imb.70002 – Name: URL Label: Availability Group: URL Data: https://research.wur.nl/en/publications/syncas-mediated-crispr-cas9-genome-editing-in-the-jewel-wasp-naso<br />https://doi.org/10.1111/imb.70002<br />https://edepot.wur.nl/698620 – Name: Copyright Label: Rights Group: Cpyrght Data: https://creativecommons.org/licenses/by/4.0/ ; Wageningen University & Research – Name: AN Label: Accession Number Group: ID Data: edsbas.280B6914 |
| PLink | https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edsbas&AN=edsbas.280B6914 |
| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1111/imb.70002 Languages: – Text: English Subjects: – SubjectFull: BAPC Type: general – SubjectFull: Nasonia vitripennis Type: general – SubjectFull: SYNCAS Type: general – SubjectFull: gene editing Type: general – SubjectFull: saponin Type: general Titles: – TitleFull: SYNCAS-mediated CRISPR-Cas9 genome editing in the Jewel wasp, Nasonia vitripennis Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Guerra, Filippo – PersonEntity: Name: NameFull: De Rouck, Sander – PersonEntity: Name: NameFull: Verhulst, Eveline C. IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 01 Type: published Y: 2026 Identifiers: – Type: issn-locals Value: edsbas – Type: issn-locals Value: edsbas.oa Titles: – TitleFull: Insect Molecular Biology 35 (2026) 1 ; ISSN: 0962-1075 Type: main |
| ResultId | 1 |