Academic Journal
Micropatterns of double-layered nanofiber scaffolds with dual functions of cell patterning and metabolite detection.
| Τίτλος: | Micropatterns of double-layered nanofiber scaffolds with dual functions of cell patterning and metabolite detection. |
|---|---|
| Συνεισφορές: | Hyun Jong Lee, Han-Soo Kim, Hyun Ok Kim, Won-Gun Koh, Kim, Hyun Ok |
| Έτος έκδοσης: | 2011 |
| Θεματικοί όροι: | Antibodies, Immobilized/immunology, Hep G2 Cells, Humans, Hydrogel, Polyethylene Glycol Dimethacrylate/chemistry, Metabolome, Microarray Analysis/instrumentation, Microarray Analysis/methods, Nanofibers/chemistry, Polyethylene Glycols/chemistry, Polystyrenes/chemistry, Serum Albumin/analysis, Serum Albumin/immunology |
| Περιγραφή: | This paper describes the development of multi-functional nanofiber scaffolds consisting of multiple layers of nanofiber scaffolds and nanofiber-incorporated poly(ethylene glycol) (PEG) hydrogels. As a proof-of-concept demonstration, we fabricated micropatterned polymeric nanofiber scaffolds that were capable of simultaneously generating cellular micropatterns within a biomimetic environment and detecting cellular metabolic products within well-defined microdomains. To achieve this goal, we designed nanofiber scaffolds with both vertical and lateral microdomains. Vertically heterogeneous structures that were responsible for multi-functionality were realized by preparing double-layered nanofiber scaffolds consisting of an antibody-immobilized bottom layer of nanofibers and an upper layer of bare polystyrene (PS) nanofibers by a two-step sequential electrospinning process. Photopatterning of poly(ethylene glycol) (PEG) hydrogel on the electrospun nanofibers produced laterally heterogeneous micropatterned nanofiber scaffolds made of hydrogel microwells filled with a nanofibrous region, which is capable of generating cell and protein micropatterns due to the different interactions that cells and proteins have with PEG hydrogels and nanofibers. When HepG2 cells were seeded into resultant nanofiber scaffolds, cells selectively adhered within the 200 μm × 200 μm PS fiber microdomain and formed 180.2 ± 6.7 μm spheroids after 5 days of culture in the upper layer. Furthermore, immobilized anti-albumin in the bottom layer detected albumin secreted by micropatterned HepG2 cells with higher sensitivity than flat PS substrates, demonstrating successful accomplishment of dual functions using micropatterned double-layered nanofiber scaffolds. ; open |
| Τύπος εγγράφου: | article in journal/newspaper |
| Περιγραφή αρχείου: | 2849~2857 |
| Γλώσσα: | unknown |
| Relation: | LAB ON A CHIP; J02148; https://ir.ymlib.yonsei.ac.kr/handle/22282913/94640; http://pubs.rsc.org/en/content/articlelanding/2011/lc/c1lc20186g; T201104129; 27469 |
| DOI: | 10.1039/C1LC20186G |
| Διαθεσιμότητα: | https://ir.ymlib.yonsei.ac.kr/handle/22282913/94640 https://doi.org/10.1039/C1LC20186G http://pubs.rsc.org/en/content/articlelanding/2011/lc/c1lc20186g |
| Rights: | CC BY-NC-ND 2.0 KR ; https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ ; not free |
| Αριθμός Καταχώρησης: | edsbas.271A54EB |
| Βάση Δεδομένων: | BASE |
| FullText | Text: Availability: 0 CustomLinks: – Url: https://ir.ymlib.yonsei.ac.kr/handle/22282913/94640# Name: EDS - BASE (ns324271) Category: fullText Text: View record from BASE |
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| Header | DbId: edsbas DbLabel: BASE An: edsbas.271A54EB RelevancyScore: 843 AccessLevel: 3 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 842.654235839844 |
| IllustrationInfo | |
| Items | – Name: Title Label: Title Group: Ti Data: Micropatterns of double-layered nanofiber scaffolds with dual functions of cell patterning and metabolite detection. – Name: Author Label: Contributors Group: Au Data: Hyun Jong Lee<br />Han-Soo Kim<br />Hyun Ok Kim<br />Won-Gun Koh<br />Kim, Hyun Ok – Name: DatePubCY Label: Publication Year Group: Date Data: 2011 – Name: Subject Label: Subject Terms Group: Su Data: <searchLink fieldCode="DE" term="%22Antibodies%22">Antibodies</searchLink><br /><searchLink fieldCode="DE" term="%22Immobilized%2Fimmunology%22">Immobilized/immunology</searchLink><br /><searchLink fieldCode="DE" term="%22Hep+G2+Cells%22">Hep G2 Cells</searchLink><br /><searchLink fieldCode="DE" term="%22Humans%22">Humans</searchLink><br /><searchLink fieldCode="DE" term="%22Hydrogel%22">Hydrogel</searchLink><br /><searchLink fieldCode="DE" term="%22Polyethylene+Glycol+Dimethacrylate%2Fchemistry%22">Polyethylene Glycol Dimethacrylate/chemistry</searchLink><br /><searchLink fieldCode="DE" term="%22Metabolome%22">Metabolome</searchLink><br /><searchLink fieldCode="DE" term="%22Microarray+Analysis%2Finstrumentation%22">Microarray Analysis/instrumentation</searchLink><br /><searchLink fieldCode="DE" term="%22Microarray+Analysis%2Fmethods%22">Microarray Analysis/methods</searchLink><br /><searchLink fieldCode="DE" term="%22Nanofibers%2Fchemistry%22">Nanofibers/chemistry</searchLink><br /><searchLink fieldCode="DE" term="%22Polyethylene+Glycols%2Fchemistry%22">Polyethylene Glycols/chemistry</searchLink><br /><searchLink fieldCode="DE" term="%22Polystyrenes%2Fchemistry%22">Polystyrenes/chemistry</searchLink><br /><searchLink fieldCode="DE" term="%22Serum+Albumin%2Fanalysis%22">Serum Albumin/analysis</searchLink><br /><searchLink fieldCode="DE" term="%22Serum+Albumin%2Fimmunology%22">Serum Albumin/immunology</searchLink> – Name: Abstract Label: Description Group: Ab Data: This paper describes the development of multi-functional nanofiber scaffolds consisting of multiple layers of nanofiber scaffolds and nanofiber-incorporated poly(ethylene glycol) (PEG) hydrogels. As a proof-of-concept demonstration, we fabricated micropatterned polymeric nanofiber scaffolds that were capable of simultaneously generating cellular micropatterns within a biomimetic environment and detecting cellular metabolic products within well-defined microdomains. To achieve this goal, we designed nanofiber scaffolds with both vertical and lateral microdomains. Vertically heterogeneous structures that were responsible for multi-functionality were realized by preparing double-layered nanofiber scaffolds consisting of an antibody-immobilized bottom layer of nanofibers and an upper layer of bare polystyrene (PS) nanofibers by a two-step sequential electrospinning process. Photopatterning of poly(ethylene glycol) (PEG) hydrogel on the electrospun nanofibers produced laterally heterogeneous micropatterned nanofiber scaffolds made of hydrogel microwells filled with a nanofibrous region, which is capable of generating cell and protein micropatterns due to the different interactions that cells and proteins have with PEG hydrogels and nanofibers. When HepG2 cells were seeded into resultant nanofiber scaffolds, cells selectively adhered within the 200 μm × 200 μm PS fiber microdomain and formed 180.2 ± 6.7 μm spheroids after 5 days of culture in the upper layer. Furthermore, immobilized anti-albumin in the bottom layer detected albumin secreted by micropatterned HepG2 cells with higher sensitivity than flat PS substrates, demonstrating successful accomplishment of dual functions using micropatterned double-layered nanofiber scaffolds. ; open – Name: TypeDocument Label: Document Type Group: TypDoc Data: article in journal/newspaper – Name: Format Label: File Description Group: SrcInfo Data: 2849~2857 – Name: Language Label: Language Group: Lang Data: unknown – Name: NoteTitleSource Label: Relation Group: SrcInfo Data: LAB ON A CHIP; J02148; https://ir.ymlib.yonsei.ac.kr/handle/22282913/94640; http://pubs.rsc.org/en/content/articlelanding/2011/lc/c1lc20186g; T201104129; 27469 – Name: DOI Label: DOI Group: ID Data: 10.1039/C1LC20186G – Name: URL Label: Availability Group: URL Data: https://ir.ymlib.yonsei.ac.kr/handle/22282913/94640<br />https://doi.org/10.1039/C1LC20186G<br />http://pubs.rsc.org/en/content/articlelanding/2011/lc/c1lc20186g – Name: Copyright Label: Rights Group: Cpyrght Data: CC BY-NC-ND 2.0 KR ; https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ ; not free – Name: AN Label: Accession Number Group: ID Data: edsbas.271A54EB |
| PLink | https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=edsbas&AN=edsbas.271A54EB |
| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1039/C1LC20186G Languages: – Text: unknown Subjects: – SubjectFull: Antibodies Type: general – SubjectFull: Immobilized/immunology Type: general – SubjectFull: Hep G2 Cells Type: general – SubjectFull: Humans Type: general – SubjectFull: Hydrogel Type: general – SubjectFull: Polyethylene Glycol Dimethacrylate/chemistry Type: general – SubjectFull: Metabolome Type: general – SubjectFull: Microarray Analysis/instrumentation Type: general – SubjectFull: Microarray Analysis/methods Type: general – SubjectFull: Nanofibers/chemistry Type: general – SubjectFull: Polyethylene Glycols/chemistry Type: general – SubjectFull: Polystyrenes/chemistry Type: general – SubjectFull: Serum Albumin/analysis Type: general – SubjectFull: Serum Albumin/immunology Type: general Titles: – TitleFull: Micropatterns of double-layered nanofiber scaffolds with dual functions of cell patterning and metabolite detection. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Hyun Jong Lee – PersonEntity: Name: NameFull: Han-Soo Kim – PersonEntity: Name: NameFull: Hyun Ok Kim – PersonEntity: Name: NameFull: Won-Gun Koh – PersonEntity: Name: NameFull: Kim, Hyun Ok IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 01 Type: published Y: 2011 Identifiers: – Type: issn-locals Value: edsbas – Type: issn-locals Value: edsbas.oa |
| ResultId | 1 |