Academic Journal
In vivo drug testing during embryonic wound healing
| Τίτλος: | In vivo drug testing during embryonic wound healing |
|---|---|
| Συγγραφείς: | Bablok, Martin (cand. med.), Brand-Saberi, Beate (Prof. Dr. rer. nat.), Gellisch, Morris (Dr. rer. nat.), Morosan-Puopolo, Gabriela (Dr.) |
| Έτος έκδοσης: | 2023 |
| Συλλογή: | Dokumentenrepositorium der RUB / RUB-Repository (Ruhr-Universität Bochum) |
| Θεματικοί όροι: | ddc:610 |
| Περιγραφή: | The relevance of identifying pathological processes in the context of embryonic development is increasingly gaining attention in terms of professionalized prenatal care. To analyze local effects of prenatally administered drugs during embryonic development, the model organism of the chicken embryo can be used in a first exploratory approach. For the examination of local dexamethasone administration — as an exemplary drug — common bead implantation protocols have been adapted to serve as an in vivo technique for local drug testing during embryonic skin regeneration. For this, acrylic beads were soaked in a dexamethasone solution and implanted into skin incisional wounds of 4-day-old chicken embryos. After further incubation, the effects of the applied substance on the process of embryonic skin regeneration were analyzed using histological and molecular biological techniques. This data descriptor contains a detailed microsurgical protocol, a representative video demonstration, and exemplary results of local glucocorticoid-induced changes during embryonic wound healing. To conclude, this method allows for the analysis of the local effects of a particular substance on a cellular level and can be extended to serve as an in vivo technique for numerous other drugs to be tested on embryonic tissue. |
| Τύπος εγγράφου: | article in journal/newspaper |
| Περιγραφή αρχείου: | application/pdf |
| Γλώσσα: | English |
| Διαθεσιμότητα: | https://hss-opus.ub.ruhr-uni-bochum.de/opus4/frontdoor/index/index/docId/11044 https://nbn-resolving.org/urn:nbn:de:hbz:294-110447 https://hss-opus.ub.ruhr-uni-bochum.de/opus4/files/11044/GellischMorris2.pdf |
| Rights: | http://creativecommons.org/licenses/by/4.0/ ; info:eu-repo/semantics/openAccess |
| Αριθμός Καταχώρησης: | edsbas.18462460 |
| Βάση Δεδομένων: | BASE |
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