Academic Journal
Multi-drug resistance and biofilm production among diarrheagenic Escherichia coli pathotypes isolated from stools of children with acute diarrheal disease ; Множественная лекарственная устойчивость и образование биопленок среди диарейных патотипов Escherichia coli, выделенных из стула детей с острой диареей
| Τίτλος: | Multi-drug resistance and biofilm production among diarrheagenic Escherichia coli pathotypes isolated from stools of children with acute diarrheal disease ; Множественная лекарственная устойчивость и образование биопленок среди диарейных патотипов Escherichia coli, выделенных из стула детей с острой диареей |
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| Συγγραφείς: | David E.E., Yameen M.A., Igwenyi I., Okafor A.C., Obeten U.N., Obasi D.O., Ezeilo U.R., Emeribole M.N., David C.N. |
| Συνεισφορές: | The World Academy of Sciences (TWAS) in collaboration with Higher Education Commission (HEC), Pakistan through COMSATS University Islamabad, Abbottabad Campus, Abbottabad, Pakistan provided funding for this work. The funding body had no role in either the design of the study, collection of samples, analysis, and interpretation of data or in writing of the script. |
| Πηγή: | Russian Journal of Infection and Immunity; Vol 11, No 5 (2021); 958-964 ; Инфекция и иммунитет; Vol 11, No 5 (2021); 958-964 ; 2313-7398 ; 2220-7619 |
| Στοιχεία εκδότη: | SPb RAACI |
| Έτος έκδοσης: | 2021 |
| Συλλογή: | Russian Journal of Infection and Immunity (Infektsiya i immunitet) / Инфекция и иммунитет |
| Θεματικοί όροι: | diarrheagenic E. coli (DEC), antibiotic resistance, diarrheal stool, multiplex PCR, multi-drug resistance, polymerase chain reaction, biofilms, диареагенная кишечная палочка (DEC), устойчивость к антибиотикам, диарейный стул, мультиплексная ПЦР, множественная лекарственная устойчивость, полимеразной цепная реакция, биопленки |
| Περιγραφή: | Background. Diarrheagenic E. coli (DEC) is an etiological agent of childhood diarrhea. Resistance against commonly used drugs in the empirical treatment of enteric infections has increased among DEC. Relationship between antibiotic resistance and biofilm formation in microorganisms have been widely reported. This study was aimed to determine the antibiotic resistance and biofilm production pattern among DEC pathotypes isolated from stools of children aged 0–5 years with acute diarrheal disease in Abakaliki, Nigeria. Materials and methods. Diarrheal stool samples were obtained from 60 children and E. coli were isolated and identified using standard guidelines provided for laboratory diagnosis of enteric pathogens. Molecular identification was done by amplification of E. coli universal stress protein A (uspA) using polymerase chain reaction (PCR) method. Detection of virulent genes of DEC pathotypes was performed in a group of multiplex PCR using their specific primers. Kirby–Bauer disk diffusion method was used to determine the antibiotic susceptibility patterns of the isolates while biofilms production was detected by thiazolyl blue tetrazolium bromide dye in a 96-well plate. Results. DEC was isolated in 40 stools among which EIEC [40% (n = 16)] was commonly detected followed by ETEC [30% (n = 12)], EAEC [20% (n = 8)] and typical EPEC [10% (n = 4)]. Half of EAEC showed the highest multidrug resistance against ampicillin, cefoxitin, ciprofloxacin, levofloxacin, and tetracycline with the strongest biofilm production followed by all the EPEC which were resistant to ampicillin, ciprofloxacin, levofloxacin, and tetracycline with moderate biofilm production. All the LT-ETEC exhibited the least resistance to ampicillin and tetracycline with the weakest biofilm production. Conclusion. High frequency of the EIEC pathotype suggests its role as the primary etiological agent of diarrhea in children. Correlation between high drug resistance and biofilm production among the pathotype may indicate that biofilms may provide ... |
| Τύπος εγγράφου: | article in journal/newspaper |
| Περιγραφή αρχείου: | application/pdf |
| Γλώσσα: | English |
| Relation: | https://iimmun.ru/iimm/article/view/1589/1340; https://iimmun.ru/iimm/article/downloadSuppFile/1589/5823; https://iimmun.ru/iimm/article/downloadSuppFile/1589/5824; https://iimmun.ru/iimm/article/downloadSuppFile/1589/5825; https://iimmun.ru/iimm/article/downloadSuppFile/1589/5826; https://iimmun.ru/iimm/article/downloadSuppFile/1589/5829; https://iimmun.ru/iimm/article/downloadSuppFile/1589/5830; https://iimmun.ru/iimm/article/downloadSuppFile/1589/6914; https://iimmun.ru/iimm/article/view/1589 |
| DOI: | 10.15789/2220-7619-MDR-1589 |
| Διαθεσιμότητα: | https://iimmun.ru/iimm/article/view/1589 https://doi.org/10.15789/2220-7619-MDR-1589 |
| Rights: | Copyright (c) 2021 David E.E., Yameen M.A., Igwenyi I., Okafor A.C., Obeten U.N., Obasi D.O., Ezeilo U.R., Emeribole M.N., David C.N. ; https://creativecommons.org/licenses/by/4.0 |
| Αριθμός Καταχώρησης: | edsbas.178E2D9C |
| Βάση Δεδομένων: | BASE |
| DOI: | 10.15789/2220-7619-MDR-1589 |
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