Academic Journal
Capturing the interactome of newly transcribed RNA
| Τίτλος: | Capturing the interactome of newly transcribed RNA |
|---|---|
| Συγγραφείς: | Bao, Xichen, Guo, Xiangpeng, Yin, Menghui, Tariq, Muqddas, Lai, Yiwei, Kanwal, Shahzina, Zhou, Jiajian, Li, Na, Lv, Yuan, Pulido-Quetglas, Carlos, Wang, Xiwei, Ji, Lu, Khan, Muhammad J, Zhu, Xihua, Luo, Zhiwei, Shao, Changwei, Lim, Do-Hwan, Liu, Xiao, Li, Nan, Wang, Wei, He, Minghui, Ward, Carl, Wang, Tong, Zhang, Gong, Wang, Dongye, Yang, Jianhua, Chen, Yiwen, Zhang, Chaolin, Jauch, Ralf, Yang, Yun-Gui, Wang, Yangming, Qin, Baoming, Anko, Minna-Liisa, Hutchins, Andrew P, Sun, Hao, Wang, Huating, Fu, Xiang-Dong, Zhang, Biliang, Esteban, Miguel A |
| Πηγή: | Bao, X, Guo, X, Yin, M, Tariq, M, Lai, Y, Kanwal, S, Zhou, J, Li, N, Lv, Y, Pulido-Quetglas, C, Wang, X, Ji, L, Khan, M J, Zhu, X, Luo, Z, Shao, C, Lim, D-H, Liu, X, Li, N, Wang, W, He, M, Ward, C, Wang, T, Zhang, G, Wang, D, Yang, J, Chen, Y, Zhang, C, Jauch, R, Yang, Y-G, Wang, Y, Qin, B, Anko, M-L, Hutchins, A P, Sun, H, Wang, H, Fu, X-D, Zhang, B & Esteban, M A 2018, 'Capturing the interactome of newly transcribed RNA', Nature Methods, vol. 15, no. 3, pp. 213-220. https://doi.org/10.1038/nmeth.4595 |
| Έτος έκδοσης: | 2018 |
| Θεματικοί όροι: | Animals, Click Chemistry/methods, Embryonic Stem Cells/cytology, HeLa Cells, High-Throughput Nucleotide Sequencing/methods, Humans, Mass Spectrometry/methods, Mice, Protein Interaction Maps, Proteome/metabolism, RNA/genetics, RNA-Binding Proteins/genetics, Uridine/analogs & derivatives |
| Περιγραφή: | We combine the labeling of newly transcribed RNAs with 5-ethynyluridine with the characterization of bound proteins. This approach, named capture of the newly transcribed RNA interactome using click chemistry (RICK), systematically captures proteins bound to a wide range of RNAs, including nascent RNAs and traditionally neglected nonpolyadenylated RNAs. RICK has identified mitotic regulators amongst other novel RNA-binding proteins with preferential affinity for nonpolyadenylated RNAs, revealed a link between metabolic enzymes/factors and nascent RNAs, and expanded the known RNA-bound proteome of mouse embryonic stem cells. RICK will facilitate an in-depth interrogation of the total RNA-bound proteome in different cells and systems. |
| Τύπος εγγράφου: | article in journal/newspaper |
| Γλώσσα: | English |
| ISSN: | 1548-7091 |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/29431736; info:eu-repo/semantics/altIdentifier/pissn/1548-7091 |
| DOI: | 10.1038/nmeth.4595 |
| Διαθεσιμότητα: | https://research.abo.fi/en/publications/4f25986c-0f2f-42cf-af2b-a6ab08a39269 https://doi.org/10.1038/nmeth.4595 |
| Rights: | info:eu-repo/semantics/closedAccess |
| Αριθμός Καταχώρησης: | edsbas.14708E66 |
| Βάση Δεδομένων: | BASE |
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