Academic Journal
Force-Dependent Interactions between Talin and Full-Length Vinculin
| Τίτλος: | Force-Dependent Interactions between Talin and Full-Length Vinculin |
|---|---|
| Συγγραφείς: | Wang, Yinan, Yao, Mingxi, Baker, Karen B, Gough, Rosemarie E, Le, Shimin, Goult, Benjamin T, Yan, Jie |
| Στοιχεία εκδότη: | American Chemical Society (ACS) |
| Έτος έκδοσης: | 2021 |
| Συλλογή: | The University of Liverpool Repository |
| Περιγραφή: | Talin and vinculin are part of a multicomponent system involved in mechanosensing in cell-matrix adhesions. Both exist in autoinhibited forms, and activation of vinculin requires binding to mechanically activated talin, yet how forces affect talin’s interaction with vinculin has not been investigated. Here by quantifying the kinetics of force-dependent talin-vinculin interactions using single-molecule analysis, we show that mechanical exposure of a single vinculin binding site (VBS) in talin is sufficient to relieve the autoinhibition of vinculin, resulting in high-affinity binding. We provide evidence that the vinculin undergoes dynamic fluctuations between an autoinhibited closed conformation and an open conformation that is stabilized upon binding to the VBS. Furthermore, we discover an additional level of regulation in which the mechanically exposed VBS binds vinculin significantly more tightly than the isolated VBS alone. Molecular dynamics simulations reveal the basis of this new regulatory mechanism, identifying a sensitive force-dependent change in the conformation of an exposed VBS that modulates binding. Together, these results provide a comprehensive understanding of how the interplay between force and autoinhibition provides exquisite complexity within this major mechanosensing axis. |
| Τύπος εγγράφου: | article in journal/newspaper |
| Περιγραφή αρχείου: | application/pdf |
| Γλώσσα: | English |
| ISSN: | 0002-7863 |
| Relation: | https://livrepository.liverpool.ac.uk/3179293/1/2021_Wang_JACS.pdf; Wang, Yinan, Yao, Mingxi, Baker, Karen B, Gough, Rosemarie E, Le, Shimin, Goult, Benjamin T orcid:0000-0002-3438-2807 and Yan, Jie (2021) Force-Dependent Interactions between Talin and Full-Length Vinculin JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 143 (36). pp. 14726-14737. ISSN 0002-7863, 1520-5126 |
| DOI: | 10.1021/jacs.1c06223 |
| Διαθεσιμότητα: | https://livrepository.liverpool.ac.uk/3179293/ https://doi.org/10.1021/jacs.1c06223 https://livrepository.liverpool.ac.uk/3179293/1/2021_Wang_JACS.pdf |
| Αριθμός Καταχώρησης: | edsbas.1283CED9 |
| Βάση Δεδομένων: | BASE |
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