Academic Journal
Изучение состояния реактивности мембраны Шнайдера при некоторых формах стоматогенного верхнечелюстного синусита
| Title: | Изучение состояния реактивности мембраны Шнайдера при некоторых формах стоматогенного верхнечелюстного синусита |
|---|---|
| Source: | ScienceRise: Medical Science; № 9 (5) (2016); 4-13 ScienceRise. Medical Science; № 9 (5) (2016); 4-13 |
| Publisher Information: | PRIVAT COMPANY "TECHNOLOGY CENTER", 2016. |
| Publication Year: | 2016 |
| Subject Terms: | патоморфологическое исследование, реактивность мембраны Шнайдера, травматический ятрогенный верхнечелюстной синусит, одонтогенный синусит, УДК 616.216-002:614.253.8:616.89-001]-076, pathomorphological study, Schniederian membrane reactivity, traumatic iatrogenic maxillary sinusitis, odontogenic sinusitis, 3. Good health |
| Description: | The one of causes of the high prevalence rates of the chronic maxillary sinusitis is an absence of differentiated approach to the treatment of the different forms of this pathology. The spread of infection on the mucous tunic of maxillary sinus takes place already after extraction of tooth with gangrenous pulp as a result of suppuration of the maxilla root cyst, at osteomyelitis of alveolar process, after operations of sinus-lifting at implantation of maxillary teeth. Standardization of treatment of inflammatory pathological states in maxillary sinus that have different causes and mechanisms of development leads to the temporal extinction of expressed clinical symptoms only, favoring the chronization of disease.Aim of research: To study morphological changes of Schneiderian membrane at odontogenic and iatrogenic (traumatic form) maxillary sinusitis of stomatogenic origin.Material and methodsThere were studied intraoperational biopsy materials of 14 (19,7 %) patients with odontogenic maxillary sinusitis (control group) and 57 (80,3 %) patients with traumatic form of iatrogenic maxillary sinusitis (main group). For the survey microscopy histological sections were colored with hematoxylin and eosin, where the height of mucous tunic epithelium, absolute area of leuko-lymphocytic and hemorrhagic infiltrates were defined, the state of vessels of microcirculatory channel was assessed. For analysis of the process of fiber-creation in studied samples, the sections were colored with Weigert hematoxylin according to Van Gieson. For qualitative and quantitative study of cells distribution in mucous tunic of maxillary sinus the morphometric net of S.B. Stephanov was used. Manasse pathomorphological classification was used for differentiation of the revealed changes in mucous tunic. For revelation of the natural killer cells, the sections were colored with alcian blue (critical concentration of magnesium chloride 0,6 Mol) with additional coloration of kernels with hematocylin. The reactions with СD 8, CD 56, CD 68, CD 138, CD 43 monoclonal antibodies were carried out. Streptavidin-biotin system of visualization of LSAB2 antibodies (peroxidate mark + benzidine) (LabVision, USA) was used. The number of CD 8+, CD 68+ cells in sight was counted. All prescriptions of solutions were taken from instructions.Photodocumentation was carried out using Axiolab binocular microscope, Axiocam digital camera with 8 megapixel matrix, personal computer, connected with digital camera by interface, video cable and «AxioVision 4.8» software. Statistical processing of the received results was carried out using tables of R.B. Strelkov, by accelerated method of quantitative comparison of morphological preparations. The reliability of the received results were assessed according to the method of Student-Fisher for reliability level no less than 95 %,that is conventional for biological and medical studies (р0,05); fibrosis of the proper plate of the mucous tunic of maxillary sinus, in several cases more expressed proliferation of collagenous fibers of the proper plate (21,4±10,9 %, р>0,05); expressed edema of the proper plate tissue with effusion in extracellular matrix (14,3±9,3 %, р>0,05); cysts (7,2 %, р>0,05). CD 8+ (8,48±0,25 %), were found in infiltrate, CD 68+ macrophages prevail (21,5±0,4 %), CD56+ cytotoxic lymphocytes (singular), CD 43+ complexes, CD 138+ cells (in insignificant number). In Schneiderian membrane epithelium were revealed desquamation (64,3±12,8 %), necrosis (57,1±13,2 %), focal planocellular metaplasia (42,9±13,2 %), vacuole dystrophy of epithelial cells (42,9±13,2 %), intraepithelial lymphocytic infiltration (35,7±10,2 %). In average epithelium height is 44,6±1,5 mcm.Traumatic form of iatrogenic maxillary sinusitis is characterized with expressed diffuse and focal mainly macrophagic-lymphocytic and hemorrhagic infiltration of the proper plate of the mucous tunic (36,8±6,3 %, р При одонтогенном синусите, отмечена выраженная сосудистая реакция, асептическое воспаление слизистой, умеренное разрастание коллагеновых волокон, нарушение рядности эпителия, очаги некроза, десквамации, плоскоклеточной метаплазии. При травматической форме ятрогенного синусита –, некроз, метаплазия и внутриэпителиальная лимфоцитарная инфильтрация. Выраженный фиброз собственной пластинки слизистой оболочки отсутствует. Возможно восстановление эпителиального покрова, обеспечивающего муко-цилиарный клиренс |
| Document Type: | Article |
| File Description: | application/pdf |
| Language: | Russian |
| ISSN: | 2519-478X 2519-4798 |
| Access URL: | http://journals.uran.ua/sr_med/article/view/77476 |
| Rights: | CC BY |
| Accession Number: | edsair.scientific.p..29a7b72efd9f72f7df9a5a26d380761f |
| Database: | OpenAIRE |
| FullText | Text: Availability: 0 CustomLinks: – Url: https://explore.openaire.eu/search/publication?articleId=scientific_p%3A%3A29a7b72efd9f72f7df9a5a26d380761f Name: EDS - OpenAIRE (ns324271) Category: fullText Text: View record at OpenAIRE – Url: https://resolver.ebsco.com/c/fiv2js/result?sid=EBSCO:edsair&genre=article&issn=2519478X&ISBN=&volume=&issue=&date=20160930&spage=&pages=&title=ScienceRise: Medical Science&atitle=%D0%98%D0%B7%D1%83%D1%87%D0%B5%D0%BD%D0%B8%D0%B5%20%D1%81%D0%BE%D1%81%D1%82%D0%BE%D1%8F%D0%BD%D0%B8%D1%8F%20%D1%80%D0%B5%D0%B0%D0%BA%D1%82%D0%B8%D0%B2%D0%BD%D0%BE%D1%81%D1%82%D0%B8%20%D0%BC%D0%B5%D0%BC%D0%B1%D1%80%D0%B0%D0%BD%D1%8B%20%D0%A8%D0%BD%D0%B0%D0%B9%D0%B4%D0%B5%D1%80%D0%B0%20%D0%BF%D1%80%D0%B8%20%D0%BD%D0%B5%D0%BA%D0%BE%D1%82%D0%BE%D1%80%D1%8B%D1%85%20%D1%84%D0%BE%D1%80%D0%BC%D0%B0%D1%85%20%D1%81%D1%82%D0%BE%D0%BC%D0%B0%D1%82%D0%BE%D0%B3%D0%B5%D0%BD%D0%BD%D0%BE%D0%B3%D0%BE%20%D0%B2%D0%B5%D1%80%D1%85%D0%BD%D0%B5%D1%87%D0%B5%D0%BB%D1%8E%D1%81%D1%82%D0%BD%D0%BE%D0%B3%D0%BE%20%D1%81%D0%B8%D0%BD%D1%83%D1%81%D0%B8%D1%82%D0%B0&aulast=&id=DOI: Name: Full Text Finder (for New FTF UI) (ns324271) Category: fullText Text: Full Text Finder MouseOverText: Full Text Finder |
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| Items | – Name: Title Label: Title Group: Ti Data: Изучение состояния реактивности мембраны Шнайдера при некоторых формах стоматогенного верхнечелюстного синусита – Name: TitleSource Label: Source Group: Src Data: ScienceRise: Medical Science; № 9 (5) (2016); 4-13<br />ScienceRise. Medical Science; № 9 (5) (2016); 4-13 – Name: Publisher Label: Publisher Information Group: PubInfo Data: PRIVAT COMPANY "TECHNOLOGY CENTER", 2016. – Name: DatePubCY Label: Publication Year Group: Date Data: 2016 – Name: Subject Label: Subject Terms Group: Su Data: <searchLink fieldCode="DE" term="%22патоморфологическое+исследование%22">патоморфологическое исследование</searchLink><br /><searchLink fieldCode="DE" term="%22реактивность+мембраны+Шнайдера%22">реактивность мембраны Шнайдера</searchLink><br /><searchLink fieldCode="DE" term="%22травматический+ятрогенный+верхнечелюстной+синусит%22">травматический ятрогенный верхнечелюстной синусит</searchLink><br /><searchLink fieldCode="DE" term="%22одонтогенный+синусит%22">одонтогенный синусит</searchLink><br /><searchLink fieldCode="DE" term="%22УДК+616%2E216-002%3A614%2E253%2E8%3A616%2E89-001]-076%22">УДК 616.216-002:614.253.8:616.89-001]-076</searchLink><br /><searchLink fieldCode="DE" term="%22pathomorphological+study%22">pathomorphological study</searchLink><br /><searchLink fieldCode="DE" term="%22Schniederian+membrane+reactivity%22">Schniederian membrane reactivity</searchLink><br /><searchLink fieldCode="DE" term="%22traumatic+iatrogenic+maxillary+sinusitis%22">traumatic iatrogenic maxillary sinusitis</searchLink><br /><searchLink fieldCode="DE" term="%22odontogenic+sinusitis%22">odontogenic sinusitis</searchLink><br /><searchLink fieldCode="DE" term="%223%2E+Good+health%22">3. Good health</searchLink> – Name: Abstract Label: Description Group: Ab Data: The one of causes of the high prevalence rates of the chronic maxillary sinusitis is an absence of differentiated approach to the treatment of the different forms of this pathology. The spread of infection on the mucous tunic of maxillary sinus takes place already after extraction of tooth with gangrenous pulp as a result of suppuration of the maxilla root cyst, at osteomyelitis of alveolar process, after operations of sinus-lifting at implantation of maxillary teeth. Standardization of treatment of inflammatory pathological states in maxillary sinus that have different causes and mechanisms of development leads to the temporal extinction of expressed clinical symptoms only, favoring the chronization of disease.Aim of research: To study morphological changes of Schneiderian membrane at odontogenic and iatrogenic (traumatic form) maxillary sinusitis of stomatogenic origin.Material and methodsThere were studied intraoperational biopsy materials of 14 (19,7 %) patients with odontogenic maxillary sinusitis (control group) and 57 (80,3 %) patients with traumatic form of iatrogenic maxillary sinusitis (main group). For the survey microscopy histological sections were colored with hematoxylin and eosin, where the height of mucous tunic epithelium, absolute area of leuko-lymphocytic and hemorrhagic infiltrates were defined, the state of vessels of microcirculatory channel was assessed. For analysis of the process of fiber-creation in studied samples, the sections were colored with Weigert hematoxylin according to Van Gieson. For qualitative and quantitative study of cells distribution in mucous tunic of maxillary sinus the morphometric net of S.B. Stephanov was used. Manasse pathomorphological classification was used for differentiation of the revealed changes in mucous tunic. For revelation of the natural killer cells, the sections were colored with alcian blue (critical concentration of magnesium chloride 0,6 Mol) with additional coloration of kernels with hematocylin. The reactions with СD 8, CD 56, CD 68, CD 138, CD 43 monoclonal antibodies were carried out. Streptavidin-biotin system of visualization of LSAB2 antibodies (peroxidate mark + benzidine) (LabVision, USA) was used. The number of CD 8+, CD 68+ cells in sight was counted. All prescriptions of solutions were taken from instructions.Photodocumentation was carried out using Axiolab binocular microscope, Axiocam digital camera with 8 megapixel matrix, personal computer, connected with digital camera by interface, video cable and «AxioVision 4.8» software. Statistical processing of the received results was carried out using tables of R.B. Strelkov, by accelerated method of quantitative comparison of morphological preparations. The reliability of the received results were assessed according to the method of Student-Fisher for reliability level no less than 95 %,that is conventional for biological and medical studies (р0,05); fibrosis of the proper plate of the mucous tunic of maxillary sinus, in several cases more expressed proliferation of collagenous fibers of the proper plate (21,4±10,9 %, р>0,05); expressed edema of the proper plate tissue with effusion in extracellular matrix (14,3±9,3 %, р>0,05); cysts (7,2 %, р>0,05). CD 8+ (8,48±0,25 %), were found in infiltrate, CD 68+ macrophages prevail (21,5±0,4 %), CD56+ cytotoxic lymphocytes (singular), CD 43+ complexes, CD 138+ cells (in insignificant number). In Schneiderian membrane epithelium were revealed desquamation (64,3±12,8 %), necrosis (57,1±13,2 %), focal planocellular metaplasia (42,9±13,2 %), vacuole dystrophy of epithelial cells (42,9±13,2 %), intraepithelial lymphocytic infiltration (35,7±10,2 %). In average epithelium height is 44,6±1,5 mcm.Traumatic form of iatrogenic maxillary sinusitis is characterized with expressed diffuse and focal mainly macrophagic-lymphocytic and hemorrhagic infiltration of the proper plate of the mucous tunic (36,8±6,3 %, р<br />При одонтогенном синусите, отмечена выраженная сосудистая реакция, асептическое воспаление слизистой, умеренное разрастание коллагеновых волокон, нарушение рядности эпителия, очаги некроза, десквамации, плоскоклеточной метаплазии. При травматической форме ятрогенного синусита –, некроз, метаплазия и внутриэпителиальная лимфоцитарная инфильтрация. Выраженный фиброз собственной пластинки слизистой оболочки отсутствует. Возможно восстановление эпителиального покрова, обеспечивающего муко-цилиарный клиренс – Name: TypeDocument Label: Document Type Group: TypDoc Data: Article – Name: Format Label: File Description Group: SrcInfo Data: application/pdf – Name: Language Label: Language Group: Lang Data: Russian – Name: ISSN Label: ISSN Group: ISSN Data: 2519-478X<br />2519-4798 – Name: URL Label: Access URL Group: URL Data: <link linkTarget="URL" linkTerm="http://journals.uran.ua/sr_med/article/view/77476" linkWindow="_blank">http://journals.uran.ua/sr_med/article/view/77476</link> – Name: Copyright Label: Rights Group: Cpyrght Data: CC BY – Name: AN Label: Accession Number Group: ID Data: edsair.scientific.p..29a7b72efd9f72f7df9a5a26d380761f |
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| RecordInfo | BibRecord: BibEntity: Languages: – Text: Russian Subjects: – SubjectFull: патоморфологическое исследование Type: general – SubjectFull: реактивность мембраны Шнайдера Type: general – SubjectFull: травматический ятрогенный верхнечелюстной синусит Type: general – SubjectFull: одонтогенный синусит Type: general – SubjectFull: УДК 616.216-002:614.253.8:616.89-001]-076 Type: general – SubjectFull: pathomorphological study Type: general – SubjectFull: Schniederian membrane reactivity Type: general – SubjectFull: traumatic iatrogenic maxillary sinusitis Type: general – SubjectFull: odontogenic sinusitis Type: general – SubjectFull: 3. Good health Type: general Titles: – TitleFull: Изучение состояния реактивности мембраны Шнайдера при некоторых формах стоматогенного верхнечелюстного синусита Type: main BibRelationships: IsPartOfRelationships: – BibEntity: Dates: – D: 30 M: 09 Type: published Y: 2016 Identifiers: – Type: issn-print Value: 2519478X – Type: issn-print Value: 25194798 – Type: issn-locals Value: edsair – Type: issn-locals Value: edsairFT Titles: – TitleFull: ScienceRise: Medical Science Type: main |
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