Λεπτομέρειες βιβλιογραφικής εγγραφής
| Τίτλος: |
Hfq influences ciprofloxacin accumulation in Escherichia coli independently of ompC and ompF post-transcriptional regulation |
| Πηγή: |
Journal of Applied Genetics. 66(2):449-457 |
| Στοιχεία εκδότη: |
2025. |
| Έτος έκδοσης: |
2025 |
| Θεματικοί όροι: |
membrane poration, antibiotic infux and efflux, ciprofoxacin, ompC and ompF porins, Hfq, small non-coding RNA |
| Περιγραφή: |
The antibiotic resistance of pathogenic bacteria is currently one of the major problems in medicine, and fnding novel antibacterial agents is one of the most difcult tasks in the feld of biomedical sciences. Studies on such tasks can be successful only if genetic and molecular mechanisms leading to antibiotic resistance/sensitivity are understood. Previous reports indicated that the bacterial protein Hfq, discovered as an RNA chaperone but subsequently demonstrated to play also other functions in cells, is involved in the mechanisms of the response of bacterial cells to antibiotics. Recently, it was found that Hfq dysfunction resulted in more efective accumulation of an antibiotic ciprofoxacin in Escherichia coli cells irrespective of the presence or absence of the AcrB efux pump. However, small RNA-mediated impairment of expression of the ompF gene, which encodes a porin involved in antibiotics infux, reversed the efects of the absence of Hfq on the antibiotic accumulation. This led to the hypothesis that Hfq might infuence ciprofoxacin accumulation in the manner independent on its RNA chaperone function, as this protein might also infuence cellular membrane structure and functions. Here, we demonstrate that in ompC and ompF mutants of E. coli, accumulation of ciprofoxacin is signifcantly impaired in the absence of Hfq or its C-terminal domain. These results corroborate the above-mentioned hypothesis on a sRNA-independent mechanism of Hfq-mediated modulation of the antibiotic transmembrane transport. Since fuoroquinolones use both protein- and lipid-mediated pathways to cross the outer membrane, Hfq may infuence both processes. This possibility will be discussed herein. |
| Τύπος εγγράφου: |
Article |
| Γλώσσα: |
English |
| ISSN: |
1234-1983 |
| DOI: |
10.1007/s13353-025-00945-910.1007/s13353-025-00945-9 |
| Αριθμός Καταχώρησης: |
edsair.dris...02463..d65468b3f58ddaf0e1c5efee5d91f5a6 |
| Βάση Δεδομένων: |
OpenAIRE |