Academic Journal
Applicability and performance of EUCAST’s rapid antimicrobial susceptibility testing (RAST) on primarily sterile body fluids in blood culture bottles in laboratory routine with total lab automation
| Τίτλος: | Applicability and performance of EUCAST’s rapid antimicrobial susceptibility testing (RAST) on primarily sterile body fluids in blood culture bottles in laboratory routine with total lab automation |
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| Συγγραφείς: | Jasuja, Jasmin Kaur, Zimmermann, Stefan, Burckhardt, Irene |
| Πηγή: | Eur J Clin Microbiol Infect Dis |
| Στοιχεία εκδότη: | Springer Science and Business Media LLC, 2021. |
| Έτος έκδοσης: | 2021 |
| Θεματικοί όροι: | 0301 basic medicine, 0303 health sciences, Bacteria, Diagnostic Tests, Routine, Bacterial Infections, Microbial Sensitivity Tests, Anti-Bacterial Agents, Body Fluids, 3. Good health, Automation, 03 medical and health sciences, Blood Culture, Humans, Original Article, Bacteria/growth, Diagnostic Tests, Routine/instrumentation [MeSH], Primarily sterile body fluids, Blood Culture [MeSH], Bacterial Infections/diagnosis [MeSH], Humans [MeSH], Microbial Sensitivity Tests/instrumentation [MeSH], Bacterial Infections/microbiology [MeSH], Bacteria/drug effects [MeSH], Blood cultures, Automation/instrumentation [MeSH], Microbial Sensitivity Tests/methods [MeSH], Laboratories [MeSH], RAST, Body Fluids/microbiology [MeSH], EUCAST, Anti-Bacterial Agents/pharmacology [MeSH], TLA, Automation/methods [MeSH], Diagnostic Tests, Routine/methods [MeSH], Laboratories |
| Περιγραφή: | Optimisation of microbiological diagnostics in primarily sterile body fluids is required. Our objective was to apply EUCAST’s RAST on primarily sterile body fluids in blood culture bottles with total lab automation (TLA) and to compare results to our reference method Vitek2 in order to report susceptibility results earlier. Positive blood culture bottles (BACTEC™ Aerobic/Anaerobic/PEDS) inoculated with primarily sterile body fluids were semi-automatically subcultured onto Columbia 5% SB agar, chocolate agar, MacConkey agar, Schaedler/KV agar and Mueller-Hinton agar. On latter, cefoxitin, ampicillin, vancomycin, piperacillin/tazobactam, meropenem and ciprofloxacin were added. After 6 h, subcultures and RAST were imaged and MALDI-TOF MS was performed. Zone sizes were digitally measured and interpreted following RAST breakpoints for blood cultures. MIC values were determined using Vitek2 panels. During a 1-year period, 197 Staphylococcus aureus, 91 Enterococcus spp., 38 Escherichia coli, 11 Klebsiella pneumoniae and 8 Pseudomonas aeruginosa were found. Categorical agreement between RAST and MIC was 96.5%. Comparison showed no very major errors, 2/7 (28.6%) and 1/7 (14.3%) of major errors for P. aeruginosa and meropenem and ciprofloxacin, 1/9 (11.1%) for K. pneumoniae and ciprofloxacin, 4/69 (7.0%) and 3/43 (5.8%) for Enterococcus spp. and vancomycin and ampicillin, respectively. Minor errors for P. aeruginosa and meropenem (1/8; 12.8%) and for E. coli and ciprofloxacin (2/29; 6.5%) were found. 30/550 RAST measurements were within area of technical uncertainty. RAST is applicable and performs well for primarily sterile body fluids in blood culture bottles, partially better than blood-based RAST. Official EUCAST evaluation is needed. |
| Τύπος εγγράφου: | Article Other literature type |
| Γλώσσα: | English |
| ISSN: | 1435-4373 0934-9723 |
| DOI: | 10.1007/s10096-020-04146-6 |
| Σύνδεσμος πρόσβασης: | https://link.springer.com/content/pdf/10.1007/s10096-020-04146-6.pdf https://pubmed.ncbi.nlm.nih.gov/33438162 https://pubmed.ncbi.nlm.nih.gov/33438162/ https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8139921 https://link.springer.com/content/pdf/10.1007/s10096-020-04146-6.pdf https://link.springer.com/article/10.1007/s10096-020-04146-6 https://www.ncbi.nlm.nih.gov/pubmed/33438162 https://repository.publisso.de/resource/frl:6448987 |
| Rights: | CC BY |
| Αριθμός Καταχώρησης: | edsair.doi.dedup.....f2e40e895ea6f8f1aa3d1b4e28f27b24 |
| Βάση Δεδομένων: | OpenAIRE |
| ISSN: | 14354373 09349723 |
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| DOI: | 10.1007/s10096-020-04146-6 |