Bibliographic Details
Title:
Development of a Multiplex Tandem PCR (MT-PCR) Assay for the Detection of Emerging SARS-CoV-2 Variants
Authors:
Richard Hale , Peter Crowley , Samir Dervisevic , Lindsay Coupland , Penelope R. Cliff , Saidat Ebie , Luke B. Snell , Joel Paul , Cheryl Williams , Paul Randell , Marcus Pond , Keith Stanley
Source:
Viruses
Publisher Information:
MDPI AG, 2021.
Publication Year:
2021
Subject Terms:
0301 basic medicine , Viral/genetics , COVID-19/diagnosis , Multiplex Polymerase Chain Reaction/methods , Coronavirus/genetics , Genome, Viral , Microbiology , Polymorphism, Single Nucleotide , Sensitivity and Specificity , 03 medical and health sciences , in vitro diagnostic test , SARS-CoV-2/genetics , Whole Genome Sequencing/methods , Humans , COVID-19 Nucleic Acid Testing/methods , Polymorphism , Nucleic Acid Amplification Techniques/methods , variants , 0303 health sciences , Genome , Whole Genome Sequencing , SARS-CoV-2 , Communication , COVID-19 , Spike Glycoprotein , QR1-502 , 3. Good health , COVID-19 Nucleic Acid Testing , Spike Glycoprotein, Coronavirus , Single Nucleotide/genetics , Multiplex Polymerase Chain Reaction , Nucleic Acid Amplification Techniques
Description:
The emergence of variants of SARS-CoV-2 has created challenges for the testing infrastructure. Although large-scale genome sequencing of SARS-CoV-2 has facilitated hospital and public health responses, access to sequencing facilities globally is variable and turnaround times can be significant, so there is a requirement for rapid and cost-effective alternatives. Applying a polymerase chain reaction (PCR)-based single nucleotide polymorphism (SNP) approach enables rapid (
Document Type:
Article
Language:
English
ISSN:
1999-4915
DOI:
10.3390/v13102028
Access URL:
https://www.mdpi.com/1999-4915/13/10/2028/pdf https://pubmed.ncbi.nlm.nih.gov/34696458 https://doaj.org/article/5e0fc06141ac4b7eb94a021dc1ffadb4 https://www.mdpi.com/1999-4915/13/10/2028 https://europepmc.org/article/MED/34696458 https://www.mendeley.com/catalogue/627feb32-9b71-3a4b-acf6-0c55a42635a3 /https://www.mdpi.com/1999-4915/13/10/2028/pdf https://search.bvsalud.org/global-literature-on-novel-coronavirus-2019-ncov/resource/ar/covidwho-1463840 https://www.ncbi.nlm.nih.gov/pubmed/34696458
Rights:
CC BY
Accession Number:
edsair.doi.dedup.....eca956479f79c32ef8c87da9c2bc2fea
Database:
OpenAIRE