Academic Journal
Characterization of the Interactions between Fluoroquinolone Antibiotics and Lipids: a Multitechnique Approach
| Τίτλος: | Characterization of the Interactions between Fluoroquinolone Antibiotics and Lipids: a Multitechnique Approach |
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| Συγγραφείς: | Bensikaddour, Hayet, Fa, Nathalie, Burton, Ingrid, Deleu, Magali, Lins, Laurence, Schanck, Andre, Brasseur, Robert, Dufrene, Yves F, Goormaghtigh, Erik, Mingeot-Leclercq, Marie*-Paule |
| Πηγή: | Biophys J |
| Στοιχεία εκδότη: | Elsevier BV, 2008. |
| Έτος έκδοσης: | 2008 |
| Θεματικοί όροι: | 0301 basic medicine, Surface Properties, Moxifloxacin, Membrane Lipids/chemistry, Biophysics, Molecular Conformation, Anti-Bacterial Agents/chemistry, Complex Mixtures -- chemistry, Fluoroquinolones -- chemistry, Phospholipids -- chemistry, Complex Mixtures, Biochimie, biophysique & biologie moléculaire, Microscopy, Atomic Force, Phase Transition, Anti-Bacterial Agents -- chemistry, Phospholipids/chemistry, Ciprofloxacin -- chemistry, Membrane Lipids, 03 medical and health sciences, Membrane Microdomains, Aza Compounds -- chemistry, Ciprofloxacin, Quinolines -- chemistry, Spectroscopy, Fourier Transform Infrared, Chimie, Microscopy, Atomic Force/methods, Ciprofloxacin/chemistry, Spectroscopy, Quinolines/chemistry, Phospholipids, Microscopy, Aza Compounds, 0303 health sciences, Fourier Transform Infrared -- methods, Membranes, Membrane Microdomains -- chemistry, Membrane Lipids -- chemistry, Membrane Microdomains/chemistry, Fluoroquinolones/chemistry, Life sciences, Aza Compounds/chemistry, Anti-Bacterial Agents, Complex Mixtures/chemistry, Spectroscopy, Fourier Transform Infrared/methods, Atomic Force -- methods, Sciences du vivant, Quinolines, Biochemistry, biophysics & molecular biology, Fluoroquinolones |
| Περιγραφή: | Probing drug/lipid interactions at the molecular level represents an important challenge in pharmaceutical research and membrane biophysics. Previous studies showed differences in accumulation and intracellular activity between two fluoroquinolones, ciprofloxacin and moxifloxacin, that may actually result from their differential susceptibility to efflux by the ciprofloxacin transporter. In view of the critical role of lipids for the drug cellular uptake and differences observed for the two closely related fluoroquinolones, we investigated the interactions of these two antibiotics with lipids, using an array of complementary techniques. Moxifloxacin induced, to a greater extent than ciprofloxacin, an erosion of the DPPC domains in the DOPC fluid phase (atomic force microscopy) and a shift of the surface pressure-area isotherms of DOPC/DPPC/fluoroquinolone monolayer toward lower area per molecule (Langmuir studies). These effects are related to a lower propensity of moxifloxacin to be released from lipid to aqueous phase (determined by phase transfer studies and conformational analysis) and a marked decrease of all-trans conformation of acyl-lipid chains of DPPC (determined by ATR-FTIR) without increase of lipid disorder and change in the tilt between the normal and the germanium surface (also determined by ATR-FTIR). All together, differences of ciprofloxacin as compared to moxifloxacin in their interactions with lipids could explain differences in their cellular accumulation and susceptibility to efflux transporters. |
| Τύπος εγγράφου: | Article Other literature type |
| Περιγραφή αρχείου: | 1 full-text file(s): application/pdf |
| Γλώσσα: | English |
| ISSN: | 0006-3495 |
| DOI: | 10.1529/biophysj.107.114843 |
| Σύνδεσμος πρόσβασης: | http://www.cell.com/article/S0006349508704612/pdf https://pubmed.ncbi.nlm.nih.gov/18178657 https://www.cell.com/fulltext/S0006-3495(08)70461-2 http://www.ncbi.nlm.nih.gov/pubmed/18178657 https://difusion.ulb.ac.be/vufind/Record/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/74189/Details http://ui.adsabs.harvard.edu/abs/2008BpJ....94.3035B/abstract http://www.sciencedirect.com/science/article/pii/S0006349508704612 https://dial.uclouvain.be/pr/boreal/object/boreal:11247 https://hdl.handle.net/2268/63309 https://doi.org/10.1529/biophysj.107.114843 |
| Rights: | Elsevier Non-Commercial CC BY NC |
| Αριθμός Καταχώρησης: | edsair.doi.dedup.....c8ed3d7f13ad4f200c05ef44708e559f |
| Βάση Δεδομένων: | OpenAIRE |
| ISSN: | 00063495 |
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| DOI: | 10.1529/biophysj.107.114843 |