Academic Journal
Probe-based confocal laser endomicroscopy and fluorescence-based enhanced reality for real-time assessment of intestinal microcirculation in a porcine model of sigmoid ischemia
| Τίτλος: | Probe-based confocal laser endomicroscopy and fluorescence-based enhanced reality for real-time assessment of intestinal microcirculation in a porcine model of sigmoid ischemia |
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| Συγγραφείς: | Diana, Michele, Dallemagne, Bernard, Chung, Hyunsoo, Nagao, Yoshihiro, Halvax, Peter, Agnus, Vincent, Soler, Luc, Lindner, Veronique, Demartines, Nicolas, Diemunsch, Pierre, Geny, Bernard, Swanström, Lee, Marescaux, Jacques |
| Πηγή: | Surgical endoscopy, vol. 28, no. 11, pp. 3224-3233 |
| Στοιχεία εκδότη: | Springer Science and Business Media LLC, 2014. |
| Έτος έκδοσης: | 2014 |
| Θεματικοί όροι: | Animals, Colon, Sigmoid/blood supply, Coloring Agents, Disease Models, Animal, Image Enhancement/methods, Indocyanine Green, Ischemia/diagnosis, Ischemia/physiopathology, Microcirculation/physiology, Microscopy, Confocal/methods, Reproducibility of Results, Swine, Microscopy, Confocal, Microcirculation, Image Enhancement, 3. Good health, 03 medical and health sciences, 0302 clinical medicine, Colon, Sigmoid, Ischemia |
| Περιγραφή: | Surgeons currently rely on visual clues to estimate the presence of sufficient vascularity for safe anastomosis. We aimed to assess the accuracy of endoluminal confocal laser endomicroscopy (CLE) and laparoscopic fluorescence-based enhanced reality (FLER), using near-infrared imaging and fluorescence from injected Indocyanine Green, to identify the transition from ischemic to vascular areas in a porcine model of mesenteric ischemia.Six pigs underwent 1-h sigmoid segmental ischemia. The ischemic area was evaluated by clinical assessment and FLER to determine presumed viable margins. For each sigmoid colon, 5 regions of interest (ROIs) were identified: ischemic (ROI 1), presumed viable margins ROI 2a (distal) and 2b (proximal), and vascular areas 3a (distal) and 3b (proximal). After injection of fluorescein, CLE scanning of the mucosa from the ischemic area toward viable margins was performed. Capillary blood samples were obtained by puncturing the serosa at the ROIs, and capillary lactates were measured with the EDGE(®) analyzer.Capillary lactates were significantly higher at ROI 1 (4.91 mmol/L) when compared to resection margins (2.8 mmol/L; mean difference: 2.11; p < 0.05) identified by FLER. There was no significant difference in lactates between ROI1 and resection margins identified by clinical evaluation. In 50 % of cases, ROI 2aCLINIC-2bCLINIC were considered to match ( |
| Τύπος εγγράφου: | Article |
| Περιγραφή αρχείου: | application/pdf |
| Γλώσσα: | English |
| ISSN: | 1432-2218 0930-2794 |
| DOI: | 10.1007/s00464-014-3595-6 |
| Σύνδεσμος πρόσβασης: | https://serval.unil.ch/resource/serval:BIB_9B6FCF8829B2.P001/REF.pdf https://pubmed.ncbi.nlm.nih.gov/24935199 https://link.springer.com/article/10.1007/s00464-014-3595-6 https://kyushu-u.pure.elsevier.com/en/publications/probe-based-confocal-laser-endomicroscopy-and-fluorescence-based- https://www.ncbi.nlm.nih.gov/pubmed/24935199 https://link.springer.com/article/10.1007/s00464-014-3595-6/fulltext.html https://pubmed.ncbi.nlm.nih.gov/24935199/ https://serval.unil.ch/notice/serval:BIB_9B6FCF8829B2 http://nbn-resolving.org/urn/resolver.pl?urn=urn:nbn:ch:serval-BIB_9B6FCF8829B27 https://serval.unil.ch/resource/serval:BIB_9B6FCF8829B2.P001/REF.pdf http://doc.rero.ch/record/325479/files/464_2014_Article_3595.pdf |
| Rights: | Springer TDM |
| Αριθμός Καταχώρησης: | edsair.doi.dedup.....bd0c4f89a63bc067e9f61c52471d0d26 |
| Βάση Δεδομένων: | OpenAIRE |
| ISSN: | 14322218 09302794 |
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| DOI: | 10.1007/s00464-014-3595-6 |