Academic Journal
EXPRESSION OF CYCLOOXYGENASE-2 IN CHOROIDAL NEOVASCULAR MEMBRANES FROM AGE-RELATED MACULAR DEGENERATION PATIENTS
| Title: | EXPRESSION OF CYCLOOXYGENASE-2 IN CHOROIDAL NEOVASCULAR MEMBRANES FROM AGE-RELATED MACULAR DEGENERATION PATIENTS |
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| Authors: | Jean-Claude Marshall, Emilia Antecka, Miguel N. Burnier, Enzo Castiglione, Shawn C. Maloney, C. Martins, Bruno F. Fernandes, Sebastian Di Cesare, Patrick Logan |
| Source: | Retina. 29:176-180 |
| Publisher Information: | Ovid Technologies (Wolters Kluwer Health), 2009. |
| Publication Year: | 2009 |
| Subject Terms: | Aged, 80 and over, Male, Membranes, Retinal Pigment Epithelium, Fibroblasts, Middle Aged, Choroidal Neovascularization, Immunoenzyme Techniques, Macular Degeneration, 03 medical and health sciences, 0302 clinical medicine, Cyclooxygenase 2, Humans, Vimentin, Female, Endothelium, Vascular, Aged |
| Description: | The objective of this study was to investigate the expression of cyclooxygenase (COX)-2 in human choroidal neovascular membranes.Paraffin-embedded sections of choroidal neovascular membranes excised from 16 patients with wet age-related macular degeneration were used for this study. Sections were subjected to immunohistochemistry using a monoclonal mouse antihuman COX-2 antibody. Staining was classified as either negative or positive in retinal pigment epithelial cells, vascular endothelial cells, and fibroblasts. Serial sections were stained for vimentin expression to confirm tissue antigenicity.Eleven of 16 (69%) choroidal neovascular membranes stained positive for COX-2 in retinal pigment epithelial cells, with 6 (38%) of these also expressing COX-2 in vascular endothelial cells and 6 (38%) in fibroblasts. None of the sections that were negative for COX-2 in the retinal pigment epithelial cells showed COX-2 expression in the other cell types assessed. There was a statistically significant difference (P = 0.0097) in the mean ages between the COX-2 positive group (65.6 years) and COX-2 negative group (76.8 years) as determined by a two-tailed, unpaired Student's t-test.The expression of COX-2 in human choroidal neovascular membranes suggests a possible role for this modulator in age-related macular degeneration pathogenesis. The age-dependent expression observed is novel and warrants further investigation. |
| Document Type: | Article |
| Language: | English |
| ISSN: | 0275-004X |
| DOI: | 10.1097/iae.0b013e3181884fa6 |
| Access URL: | https://pubmed.ncbi.nlm.nih.gov/18827739 https://www.ncbi.nlm.nih.gov/pubmed/18827739 https://europepmc.org/article/MED/18827739 |
| Accession Number: | edsair.doi.dedup.....a01eb486a3f89a5749d2597105c6d29c |
| Database: | OpenAIRE |
| ISSN: | 0275004X |
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| DOI: | 10.1097/iae.0b013e3181884fa6 |