Book
Rapid Preparation of Living Drosophila Pupal Macrophages for Ex Vivo Imaging
| Title: | Rapid Preparation of Living Drosophila Pupal Macrophages for Ex Vivo Imaging |
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| Authors: | Karling, Tua, Weavers, Helen |
| Source: | Methods in Molecular Biology ISBN: 9781071638330 |
| Publisher Information: | Springer US, 2024. |
| Publication Year: | 2024 |
| Subject Terms: | Macrophages, Pupa, Macrophages/cytology, Cell Separation, Flow Cytometry, Pupa/cytology, Flow Cytometry/methods, Drosophila melanogaster, Drosophila melanogaster/cytology, Animals, Drosophila, Cell Separation/methods |
| Description: | The fruit fly Drosophila is a well-established invertebrate model that enables in vivo imaging of innate immune cell (e.g., macrophage) migration and signaling at high spatiotemporal resolution within the intact, living animal. While optimized methods already exist to enable flow cytometry-based macrophage isolation from Drosophila at various stages of development, there remains a need for more rapid and gentle methods to isolate living macrophages for downstream ex vivo applications. Here, we describe techniques for rapid and direct isolation of living macrophages from mature Drosophila pupae and their downstream ex vivo preparation for live imaging and immunostaining. This strategy enables straightforward access to physiologically relevant innate immune cells, both circulating and tissue-resident populations, for subsequent imaging of signal transduction. |
| Document Type: | Part of book or chapter of book Article |
| Language: | English |
| DOI: | 10.1007/978-1-0716-3834-7_1 |
| Access URL: | https://pubmed.ncbi.nlm.nih.gov/38709473 |
| Rights: | Springer Nature TDM |
| Accession Number: | edsair.doi.dedup.....8ecb5e88be429a1440c0803f88b466d6 |
| Database: | OpenAIRE |
| DOI: | 10.1007/978-1-0716-3834-7_1 |
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