Academic Journal
Usefulness of an integrated microfluidic device (HPLC‐Chip‐MS) to enhance confidence in protein identification by proteomics
| Title: | Usefulness of an integrated microfluidic device (HPLC‐Chip‐MS) to enhance confidence in protein identification by proteomics |
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| Authors: | Hardouin, Julie, Duchateau, Magalie, Joubert-Caron, Raymonde, Caron, Michel |
| Contributors: | Duchateau, Magalie, Laboratoire de Biophysique Moléculaire Cellulaire et Tissulaire (BIOMOCETI), Université Paris 13 (UP13)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS) |
| Source: | Rapid Communications in Mass Spectrometry. 20:3236-3244 |
| Publisher Information: | Wiley, 2006. |
| Publication Year: | 2006 |
| Subject Terms: | Proteomics, Spectrometry, Mass, Electrospray Ionization, Proteome, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, MESH: Equipment Failure Analysis, MESH: Chromatography, MESH: Spectrometry, MESH: Peptide Mapping, Peptide Mapping, Sensitivity and Specificity, 01 natural sciences, [SDV.BBM] Life Sciences [q-bio]/Biochemistry, Molecular Biology, Nanotechnology, MESH: Nanotechnology, Molecular Biology, Chromatography, High Pressure Liquid, MESH: Proteomics, Electrospray Ionization, Reproducibility of Results, Equipment Design, Mass, Microfluidic Analytical Techniques, MESH: Sensitivity and Specificity, 0104 chemical sciences, MESH: Reproducibility of Results, MESH: Proteome, Equipment Failure Analysis, Systems Integration, High Pressure Liquid, MESH: Microfluidic Analytical Techniques, MESH: Systems Integration, MESH: Equipment Design |
| Description: | Nanoflow liquid chromatography/mass spectrometry (nanoLC/MS) has become a current tool in proteomics applications increasingly used in the search for new biomarkers. A new integrated microfluidic device (HPLC‐Chip), coupled to ion trap mass spectrometry (ITMS), appears as an innovative and robust tool for improving the identifications commonly performed by nanoLC/MS/MS. We tested this device for the identification of proteins obtained from two‐dimensional gel electrophoresis or chromatography. The chip allows the measurement of reproducible retention times that, in association with m/z ratios, was found useful for identifying peptide sequences without ambiguity. A sensitivity increase of a factor of at least 5‐fold is obtained compared to the results obtained previously in our laboratory by conventional nanoLC/MS/MS on the same ion trap. We conclude that this recently available microfluidic device can be a valuable tool during biomarker discovery programs, particularly identifying low‐abundance proteins. Copyright © 2006 John Wiley & Sons, Ltd. |
| Document Type: | Article |
| Language: | English |
| ISSN: | 1097-0231 0951-4198 |
| DOI: | 10.1002/rcm.2725 |
| Access URL: | https://pubmed.ncbi.nlm.nih.gov/17016832 http://www.ncbi.nlm.nih.gov/pubmed/17016832 https://pubmed.ncbi.nlm.nih.gov/17016832/ https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/rcm.2725 https://europepmc.org/article/MED/17016832 https://hal.archives-ouvertes.fr/hal-03157413 https://ui.adsabs.harvard.edu/abs/2006RCMS...20.3236H/abstract |
| Rights: | Wiley Online Library User Agreement |
| Accession Number: | edsair.doi.dedup.....51b93dd4161dbb58e9ce4d0e88708e30 |
| Database: | OpenAIRE |
| ISSN: | 10970231 09514198 |
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| DOI: | 10.1002/rcm.2725 |