Academic Journal
Detection of Helicobacter pylori in cow's milk.
| Τίτλος: | Detection of Helicobacter pylori in cow's milk. |
|---|---|
| Συγγραφείς: | Fujimura, S., Kawamura, T., Kato, S., Tateno, H., Watanabe, A. |
| Πηγή: | Letters in Applied Microbiology; Dec2002, Vol. 35 Issue 6, p504-507, 4p |
| Θεματικοί όροι: | Helicobacter pylori, Milk, Cows |
| Περίληψη: | Aims: To investigate the existence of Helicobacter pylori in cow's milk as one of the foods which most Japanese children eat. Methods and Results: Detection of H. pylori was demonstrated by the semi-nested polymerase chain reaction (PCR), a culture method and electron microscopy. Semi-nested PCR demonstrated the ureA gene of H. pylori in 13 of 18 (72·2%) raw milk samples and in 11 of 20 (55%) commercial pasteurized milk samples. Helicobacter pylori binding immunomagnetic beads with H. pylori -specific goat anti-H. pylori antibody was shown by electron microscopy in both raw and pasteurized milk positive for the ureA gene. Helicobacter pylori was cultured in one raw milk sample, whereas it was not cultured in pasteurized milk samples. Conclusions, Significance and Impact of the Study: There is a possibility that cow's milk is a transmission vehicle in childhood H. pylori infection, although we failed to confirm the survival of H. pylori in pasteurized milk. [ABSTRACT FROM AUTHOR] |
| Copyright of Letters in Applied Microbiology is the property of Oxford University Press / USA and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Βάση Δεδομένων: | Complementary Index |
| FullText | Links: – Type: other Text: Availability: 0 |
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| Header | DbId: edb DbLabel: Complementary Index An: 8592277 RelevancyScore: 832 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 831.506408691406 |
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| Items | – Name: Title Label: Title Group: Ti Data: Detection of Helicobacter pylori in cow's milk. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Fujimura%2C+S%2E%22">Fujimura, S.</searchLink><br /><searchLink fieldCode="AR" term="%22Kawamura%2C+T%2E%22">Kawamura, T.</searchLink><br /><searchLink fieldCode="AR" term="%22Kato%2C+S%2E%22">Kato, S.</searchLink><br /><searchLink fieldCode="AR" term="%22Tateno%2C+H%2E%22">Tateno, H.</searchLink><br /><searchLink fieldCode="AR" term="%22Watanabe%2C+A%2E%22">Watanabe, A.</searchLink> – Name: TitleSource Label: Source Group: Src Data: Letters in Applied Microbiology; Dec2002, Vol. 35 Issue 6, p504-507, 4p – Name: Subject Label: Subject Terms Group: Su Data: <searchLink fieldCode="DE" term="%22Helicobacter+pylori%22">Helicobacter pylori</searchLink><br /><searchLink fieldCode="DE" term="%22Milk%22">Milk</searchLink><br /><searchLink fieldCode="DE" term="%22Cows%22">Cows</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Aims: To investigate the existence of Helicobacter pylori in cow's milk as one of the foods which most Japanese children eat. Methods and Results: Detection of H. pylori was demonstrated by the semi-nested polymerase chain reaction (PCR), a culture method and electron microscopy. Semi-nested PCR demonstrated the ureA gene of H. pylori in 13 of 18 (72·2%) raw milk samples and in 11 of 20 (55%) commercial pasteurized milk samples. Helicobacter pylori binding immunomagnetic beads with H. pylori -specific goat anti-H. pylori antibody was shown by electron microscopy in both raw and pasteurized milk positive for the ureA gene. Helicobacter pylori was cultured in one raw milk sample, whereas it was not cultured in pasteurized milk samples. Conclusions, Significance and Impact of the Study: There is a possibility that cow's milk is a transmission vehicle in childhood H. pylori infection, although we failed to confirm the survival of H. pylori in pasteurized milk. [ABSTRACT FROM AUTHOR] – Name: Abstract Label: Group: Ab Data: <i>Copyright of Letters in Applied Microbiology is the property of Oxford University Press / USA and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1046/j.1472-765X.2002.01229.x Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 4 StartPage: 504 Subjects: – SubjectFull: Helicobacter pylori Type: general – SubjectFull: Milk Type: general – SubjectFull: Cows Type: general Titles: – TitleFull: Detection of Helicobacter pylori in cow's milk. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Fujimura, S. – PersonEntity: Name: NameFull: Kawamura, T. – PersonEntity: Name: NameFull: Kato, S. – PersonEntity: Name: NameFull: Tateno, H. – PersonEntity: Name: NameFull: Watanabe, A. IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 12 Text: Dec2002 Type: published Y: 2002 Identifiers: – Type: issn-print Value: 02668254 Numbering: – Type: volume Value: 35 – Type: issue Value: 6 Titles: – TitleFull: Letters in Applied Microbiology Type: main |
| ResultId | 1 |