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A test strip for the estimation of urea in serum.

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Title: A test strip for the estimation of urea in serum.
Authors: Kumar, Hemant, Kumar, Ashok, Kumari, Poonam, Tulsani, N.
Source: Indian Journal of Clinical Biochemistry; Jul2000, Vol. 15 Issue 2, p124-127, 4p
Abstract: We have developed a biostrip for determination of urea in serum. The test strip is based on enzymatic assay where urease has been immobilized on the chromatographic paper along with chromogen, phenol red. The chromogen is easily soluble in water and does not require other components for the color change. Serum urea reacts with urease and water to liberate ammonia and carbon dioxide. The liberated ammonia changes the pH of the reaction medium, which is monitored by the chromogen phenol red. A single step working reagent strip has been developed and the reaction is completed within 50 seconds at room temperature. With this test strip urea concentration is measured in serum as low as 0.15 g/L. The speed and convenience of determining urea in serum by this strip instantly makes it well suited for individuals, physicians and emergency centres. [ABSTRACT FROM AUTHOR]
Copyright of Indian Journal of Clinical Biochemistry is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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  – Url: https://dx.doi.org/doi:10.1007/BF02883740
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  Data: A test strip for the estimation of urea in serum.
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  Data: <searchLink fieldCode="AR" term="%22Kumar%2C+Hemant%22">Kumar, Hemant</searchLink><br /><searchLink fieldCode="AR" term="%22Kumar%2C+Ashok%22">Kumar, Ashok</searchLink><br /><searchLink fieldCode="AR" term="%22Kumari%2C+Poonam%22">Kumari, Poonam</searchLink><br /><searchLink fieldCode="AR" term="%22Tulsani%2C+N%2E%22">Tulsani, N.</searchLink>
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  Data: Indian Journal of Clinical Biochemistry; Jul2000, Vol. 15 Issue 2, p124-127, 4p
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: We have developed a biostrip for determination of urea in serum. The test strip is based on enzymatic assay where urease has been immobilized on the chromatographic paper along with chromogen, phenol red. The chromogen is easily soluble in water and does not require other components for the color change. Serum urea reacts with urease and water to liberate ammonia and carbon dioxide. The liberated ammonia changes the pH of the reaction medium, which is monitored by the chromogen phenol red. A single step working reagent strip has been developed and the reaction is completed within 50 seconds at room temperature. With this test strip urea concentration is measured in serum as low as 0.15 g/L. The speed and convenience of determining urea in serum by this strip instantly makes it well suited for individuals, physicians and emergency centres. [ABSTRACT FROM AUTHOR]
– Name: Abstract
  Label:
  Group: Ab
  Data: <i>Copyright of Indian Journal of Clinical Biochemistry is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Text: English
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      – TitleFull: A test strip for the estimation of urea in serum.
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              Text: Jul2000
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              Y: 2000
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