Academic Journal
Genomic Analysis of the Type VII Secretion System (T7SSb) Proteins in Staphylococcus aureus Isolates from Raw Meat, Processed Meat Products and Veterinary Samples of Food-Producing Animals.
| Τίτλος: | Genomic Analysis of the Type VII Secretion System (T7SSb) Proteins in Staphylococcus aureus Isolates from Raw Meat, Processed Meat Products and Veterinary Samples of Food-Producing Animals. |
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| Συγγραφείς: | Atanda, Abimbola Comfort, Matle, Itumeleng, Malesa, Refiloe, Tabit, Frederick Tawi, Mafuna, Thendo |
| Πηγή: | Bacteria (2674-1334); Sep2026, Vol. 5 Issue 3, p55, 15p |
| Θεματικοί όροι: | Staphylococcus aureus, Microbial virulence, Genomics, Food animals, Membrane proteins, Whole genome sequencing |
| Περίληψη: | Staphylococcus aureus (S. aureus) produces an array of virulence factors (VFs), which contribute to its pathogenicity. One of the VFs is the type VII secretion system (T7SS), a protein secretion apparatus present in many Gram-positive bacteria, which plays a major role during the onset of infection. This study aimed to investigate the presence of secreted effector proteins and core structural proteins of the T7SS of S. aureus isolated from raw meat, processed meat products, and veterinary samples of food-producing animals. Whole-genome sequencing (WGS) and bioinformatics tools were employed to analyze S. aureus (n = 27) isolates. The results revealed that all 27 isolates investigated harboured various genes encoded by the T7SSb: secreted effector proteins (EsxA and EsxB) and core structural proteins (EssA, EssB, EssC, EsaA, and EsaB). The presence and absence of certain genes were observed, but overall, all these isolates harboured the major protein EssC. The EssC C-terminal found in these isolates was found to be conserved, and they clustered between the EssC1 and EssC3 variants. Ramachandran plot validations and predictions indicated the stability and likely operating conservation of T7SSb proteins. An analysis of protein–protein interactions, transmembrane domains, and secondary structures offered a thorough understanding of T7SSb assembly and how various proteins interact with each other. The results revealed that the isolates contained an array of T7SSb proteins, indicating the presence of proteins associated with various human infections, which highlights the practical application of a continuous surveillance monitoring programme and investigation of S. aureus strains. [ABSTRACT FROM AUTHOR] |
| Copyright of Bacteria (2674-1334) is the property of MDPI and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Βάση Δεδομένων: | Complementary Index |
| FullText | Text: Availability: 0 |
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| Header | DbId: edb DbLabel: Complementary Index An: 197448724 RelevancyScore: 1082 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 1082.42749023438 |
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| Items | – Name: Title Label: Title Group: Ti Data: Genomic Analysis of the Type VII Secretion System (T7SSb) Proteins in Staphylococcus aureus Isolates from Raw Meat, Processed Meat Products and Veterinary Samples of Food-Producing Animals. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Atanda%2C+Abimbola+Comfort%22">Atanda, Abimbola Comfort</searchLink><br /><searchLink fieldCode="AR" term="%22Matle%2C+Itumeleng%22">Matle, Itumeleng</searchLink><br /><searchLink fieldCode="AR" term="%22Malesa%2C+Refiloe%22">Malesa, Refiloe</searchLink><br /><searchLink fieldCode="AR" term="%22Tabit%2C+Frederick+Tawi%22">Tabit, Frederick Tawi</searchLink><br /><searchLink fieldCode="AR" term="%22Mafuna%2C+Thendo%22">Mafuna, Thendo</searchLink> – Name: TitleSource Label: Source Group: Src Data: Bacteria (2674-1334); Sep2026, Vol. 5 Issue 3, p55, 15p – Name: Subject Label: Subject Terms Group: Su Data: <searchLink fieldCode="DE" term="%22Staphylococcus+aureus%22">Staphylococcus aureus</searchLink><br /><searchLink fieldCode="DE" term="%22Microbial+virulence%22">Microbial virulence</searchLink><br /><searchLink fieldCode="DE" term="%22Genomics%22">Genomics</searchLink><br /><searchLink fieldCode="DE" term="%22Food+animals%22">Food animals</searchLink><br /><searchLink fieldCode="DE" term="%22Membrane+proteins%22">Membrane proteins</searchLink><br /><searchLink fieldCode="DE" term="%22Whole+genome+sequencing%22">Whole genome sequencing</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Staphylococcus aureus (S. aureus) produces an array of virulence factors (VFs), which contribute to its pathogenicity. One of the VFs is the type VII secretion system (T7SS), a protein secretion apparatus present in many Gram-positive bacteria, which plays a major role during the onset of infection. This study aimed to investigate the presence of secreted effector proteins and core structural proteins of the T7SS of S. aureus isolated from raw meat, processed meat products, and veterinary samples of food-producing animals. Whole-genome sequencing (WGS) and bioinformatics tools were employed to analyze S. aureus (n = 27) isolates. The results revealed that all 27 isolates investigated harboured various genes encoded by the T7SSb: secreted effector proteins (EsxA and EsxB) and core structural proteins (EssA, EssB, EssC, EsaA, and EsaB). The presence and absence of certain genes were observed, but overall, all these isolates harboured the major protein EssC. The EssC C-terminal found in these isolates was found to be conserved, and they clustered between the EssC1 and EssC3 variants. Ramachandran plot validations and predictions indicated the stability and likely operating conservation of T7SSb proteins. An analysis of protein–protein interactions, transmembrane domains, and secondary structures offered a thorough understanding of T7SSb assembly and how various proteins interact with each other. The results revealed that the isolates contained an array of T7SSb proteins, indicating the presence of proteins associated with various human infections, which highlights the practical application of a continuous surveillance monitoring programme and investigation of S. aureus strains. [ABSTRACT FROM AUTHOR] – Name: Abstract Label: Group: Ab Data: <i>Copyright of Bacteria (2674-1334) is the property of MDPI and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.3390/bacteria5030055 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 15 StartPage: 55 Subjects: – SubjectFull: Staphylococcus aureus Type: general – SubjectFull: Microbial virulence Type: general – SubjectFull: Genomics Type: general – SubjectFull: Food animals Type: general – SubjectFull: Membrane proteins Type: general – SubjectFull: Whole genome sequencing Type: general Titles: – TitleFull: Genomic Analysis of the Type VII Secretion System (T7SSb) Proteins in Staphylococcus aureus Isolates from Raw Meat, Processed Meat Products and Veterinary Samples of Food-Producing Animals. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Atanda, Abimbola Comfort – PersonEntity: Name: NameFull: Matle, Itumeleng – PersonEntity: Name: NameFull: Malesa, Refiloe – PersonEntity: Name: NameFull: Tabit, Frederick Tawi – PersonEntity: Name: NameFull: Mafuna, Thendo IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 09 Text: Sep2026 Type: published Y: 2026 Identifiers: – Type: issn-print Value: 26741334 Numbering: – Type: volume Value: 5 – Type: issue Value: 3 Titles: – TitleFull: Bacteria (2674-1334) Type: main |
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